Flavonoids Protect Against T-2 Mycotoxins both in vitro and in vivo

Markham, Robert; Erhardt, Nancy; DiNinno, V L; Penman, David J.; Bhatti, A. R.

doi:10.1099/00221287-133-6-1589

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…Accordingly, these fractions exhibited a DPPH IC 50 lower than 40 µg·mL −1 and total antioxidant capacity higher than 300 mg AAE·g −1 DW. Interestingly, these fractions all exhibited a strong protective action on bovine and porcine cells upon T2 or zearalenone exposure, suggesting that this effect is at least due to the relevant phenolic compounds present in these fractions, as it has been already reported [ 39 , 40 , 41 ]. Accordingly, Montibus et al [ 42 ] showed that the phenolic composition of a maritime pine extract, and particularly methylated compounds, would be essential for its antifungal and anti-mycotoxin properties.…”

Section: Discussionsupporting

confidence: 58%

Evaluation of the Antioxidant, Anti-Inflammatory and Cytoprotective Activities of Halophyte Extracts against Mycotoxin Intoxication

Hymery

Dauvergne²,

Boussaden³

et al. 2021

Toxins

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Twelve halophyte species belonging to different families, widely represented along French Atlantic shoreline and commonly used in traditional medicine, were screened for protective activities against mycotoxins, in order to set out new promising sources of natural ingredients for feed applications. Selected halophytic species from diverse natural habitats were examined for their in vitro anti-mycotoxin activities, through viability evaluation of Madin-Darby Bovine Kidney (MDBK) and intestinal porcine enterocyte (IPEC-J2) cell lines. Besides, the in vitro antioxidant activities of plant extracts were assessed (total antioxidant and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-scavenging bioassays). Of the 12 species, Galium arenarium, Convolvulus soldanella and Eryngium campestre exhibited the most protective action on MDBK and IPEC-J2 cells against zearalenone (ZEN) or T2 toxin contamination (restoring about 75% of cell viability at 10 μg·mL−1) without inflammation response. They also had strong antioxidant capacities (Inhibitory concentration of 50% (IC50) < 100 μg·mL−1 for DPPH radical and total antioxidant capacity (TAC) of 100 to 200 mg Ascorbic Acid Equivalent (AAE)·g−1 Dry Weight), suggesting that cell protection against intoxication involves antioxidant action. A bio-guided study showed that fractions of G. arenarium extract protect MDBK cells against T2 or ZEN toxicity and several major compounds like chlorogenic acid and asperuloside could be involved in this protective effect. Overall, our results show that the halophytes G. arenarium, C. soldanella and E. campestre should be considered further as new sources of ingredients for livestock feed with protective action against mycotoxin intoxication.

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Section: Discussionsupporting

confidence: 58%

Evaluation of the Antioxidant, Anti-Inflammatory and Cytoprotective Activities of Halophyte Extracts against Mycotoxin Intoxication

Hymery

Dauvergne²,

Boussaden³

et al. 2021

Toxins

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“…Similar results were obtained by other authors. An in vitro study (Markham et al, 1986) on thymocytes revealed that quercetin offered significant protection against cytotoxicity of T-2 toxin by reducing mortality. This protection was greatest when the drug was added before or at the same time as T-2 mycotoxin to the thymocyte…”

Section: Discussionmentioning

confidence: 99%

Proliferation and apoptosis in the rabbit ovary after administration of T-2 toxin and quercetin

Leśniak-Walentyn¹,

Kolesárová²,

Medvedova³

et al. 2013

J. Anim. Feed Sci.

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“…Quercetin (3, 3′, 4′, 5, 7-penthydroxyflavone) is semi-lipophilic flavonol plentifully present in leafy vegetables, citrus fruits, tomatoes, berries, etc. It possesses several therapeutic effects such as antioxidant, anti-inflammatory, wound healing, antithrombotic, antitumor, antiprotozoal [124][125][126][127][128], and cytoprotective effects [129]. Among the different nanocarriers, polymeric nanoparticles may provide an effective way to encapsulate quercetin as they can improve bioavailability, therapeutic effectiveness, and in vivo stability.…”

Section: Quercetinmentioning

confidence: 99%

Polymeric Nanoparticles for Delivery of Natural Bioactive Agents: Recent Advances and Challenges

et al. 2023

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In the last few decades, several natural bioactive agents have been widely utilized in the treatment and prevention of many diseases owing to their unique and versatile therapeutic effects, including antioxidant, anti-inflammatory, anticancer, and neuroprotective action. However, their poor aqueous solubility, poor bioavailability, low GIT stability, extensive metabolism as well as short duration of action are the most shortfalls hampering their biomedical/pharmaceutical applications. Different drug delivery platforms have developed in this regard, and a captivating tool of this has been the fabrication of nanocarriers. In particular, polymeric nanoparticles were reported to offer proficient delivery of various natural bioactive agents with good entrapment potential and stability, an efficiently controlled release, improved bioavailability, and fascinating therapeutic efficacy. In addition, surface decoration and polymer functionalization have opened the door to improving the characteristics of polymeric nanoparticles and alleviating the reported toxicity. Herein, a review of the state of knowledge on polymeric nanoparticles loaded with natural bioactive agents is presented. The review focuses on frequently used polymeric materials and their corresponding methods of fabrication, the needs of such systems for natural bioactive agents, polymeric nanoparticles loaded with natural bioactive agents in the literature, and the potential role of polymer functionalization, hybrid systems, and stimuli-responsive systems in overcoming most of the system drawbacks. This exploration may offer a thorough idea of viewing the polymeric nanoparticles as a potential candidate for the delivery of natural bioactive agents as well as the challenges and the combating tools used to overcome any hurdles.

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Flavonoids Protect Against T-2 Mycotoxins both in vitro and in vivo

Abstract: Quercetin was able to reduce the cytotoxic effect of T-2 mycotoxin on cultured murine thymocytes. When given to mice immediately before challenge with T-2 mycotoxins, quercetin significantly reduced mortality.

Cited by 6 publications

References 14 publications

Evaluation of the Antioxidant, Anti-Inflammatory and Cytoprotective Activities of Halophyte Extracts against Mycotoxin Intoxication

Evaluation of the Antioxidant, Anti-Inflammatory and Cytoprotective Activities of Halophyte Extracts against Mycotoxin Intoxication

Proliferation and apoptosis in the rabbit ovary after administration of T-2 toxin and quercetin

Polymeric Nanoparticles for Delivery of Natural Bioactive Agents: Recent Advances and Challenges

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