Flavonol and Proanthocyanidin Glycosides from the Leaves of Artocarpus tonkinensis

Artocarpus tonkinensis Extract Inhibits LPS-Triggered Inflammation Markers and Suppresses RANKL-Induced Osteoclastogenesis in RAW264.7

“…Medicinal properties of leaf and root extracts from A. tonkinensis have long been known and they are recorded in the textbooks of traditional Vietnamese and Chinese medicine. In particular, A. tonkinensis dried leaf decoction and its major active compounds auronol glycosides maesopsin 4-O-β-D-glucoside (TAT-2) and alphitonin-4-O-β-D-glucoside (TAT-6) were studied for their immunosuppressive activity in autoimmune diseases (Thuy et al, 2004;Thuy et al, 2017) and for antiproliferative effect in anti-cancer trials (Thuy et al, 2016). Vietnamese folk medicine practice to assume A. tonkinensis decoction against rheumatoid arthritis (RA) symptoms and several studies on experimental models of collagen-induced arthritis (CIA) (Ngoc et al, 2005;Dang et al, 2009;Adorisio et al, 2019) support the awareness of A. tonkinensis efficacy to contrast chronic inflammation, and destruction of bone and joints characterizing RA pathological picture.…”

Artocarpus tonkinensis Protects Mice Against Collagen-Induced Arthritis and Decreases Th17 Cell Function

“…The medicinal use of its dried leaf decoction was discovered by Pham Chuk Lam and later studied for its immunosuppressive activity in arthritis, myasthenia gravis, and skin transplants. Different active compounds have been isolated from A. tonkinensis leaves (Thuy et al, 2017), such as the anti-arthritic n -butanol extract containing the immunosuppressive auronol glycosides maesopsin 4- O -glucoside and alphitonin-4- O -glucoside (Thuy et al, 2004). Intraperitoneal injection of its ethyl acetate extract decreased both arthritis incidence and severity and delayed disease onset in rats with CIA.…”

Investigating the Anti-Inflammatory Activity of an Ethanolic Extract From Artocarpus Tonkinensis Leaves Using a Collagen Antibody-Induced Arthritic Mouse Model

“…To obtain the purified flavonoids for the chemical structural analysis, a large-scale isolation was carried out as previously described and their structures were confirmed by comparing their ESI-MS, 1 H and 13 C NMR data with reported data [6,8].AT2 was fractionated by a classical chromatography methodology. Columns packed with DIAION HP-20 and Sephadex LH-20 adsorbents were efficient for the isolation of nine compounds.…”

“…Columns packed with DIAION HP-20 and Sephadex LH-20 adsorbents were efficient for the isolation of nine compounds. Two auronolglucosidemaesopsin 4-O--glucopyranoside (hovetrichoside C, 1), alphitonin-O--glucopyranoside (2), as well as kaempferol(3),astragalin (4), kaempferol 3-O--rutinoside (5), kaempferol 3-O-neohesperidoside(6), quercetin, quercetin 3-O--glucopyranoside (7),afzelechin-( 48″)catechin-3-O--glucopyranoside(8) and catechin (9) were identified [6,8]. The AT2 extract was analyzed for the presence of various phytochemical constituents employing screening TLC.…”

“…The decoction of its leaves and roots is used in traditional knowledgeto treat backache, arthritis and joint disorders. Our previous phytochemical analysis of the A. tonkinensisleaves revealed the presence of flavonoid compounds such as auronol, kaempferol, quercetin and their glycosides [6][7][8].Of them, two auronolglucosides (1 and 2) were found to have potent immunomodulatory, anti-proliferative and anti-inflammatory activities [9][10][11].For a possible use of 70% ethanolic extract of A. tonkinensisleaves as alternative herb medicines to treat human disease, this study wasundertaken to evaluate its possible anti-inflammatory activities in vitro using LPS-stimulated RAW 264.7 macrophages and in vivo using a collagen antibody-induced arthritic BALB/c mouse model.…”