Flavonol glycosides and antioxidant capacity of various blackberry and blueberry genotypes determined by high‐performance liquid chromatography/mass spectrometry

Abstract

Flavonol glycoside composition and content in blueberry and blackberry extracts were determined using a high-performance liquid chromatographic (HPLC) separation method coupled with photodiode array (PDA) and mass spectrometric (MS) detection. The hydrophilic antioxidant capacities of crude and fractionated flavonol extracts were also determined by the oxygen radical-absorbing capacity (ORAC FL ) and photochemiluminescence (PCL) assays. Eight flavonols of quercetin and quercetin-sugar conjugates were identified in Kiowa blackberry, namely rutinoside, galactoside, methoxyhexoside, glucoside, pentoside, [6 -(3-hydroxy-3-methylglutaroyl)]-β-galactoside, glucosylpentoside and oxalylpentoside. Thirteen flavonols were detected in Ozarkblue blueberry. Of these, myricetin 3-hexoside and 12 quercetin-sugar conjugates, namely rutinoside, galactoside, methoxyhexoside, glucoside, pentoside, glucosylpentoside, caffeoylglucoside, oxalylpentoside, rhamnoside, dimethoxyrhamnoside, acetylgalactoside and acetylglucoside, were identified. In Bluecrop blueberry, two additional quercetin-sugar conjugates were identified, namely glucuronide and caffeoylgalactoside. Quercetin glycosides accounted for 75% of total flavonols in the blueberry genotypes. Total flavonol contents ranged from 99 to 150 mg kg −1 for blackberries and from 192 to 320 mg kg −1 for blueberries. Quenching of peroxyl and superoxide anion radicals by the flavonol fractions ranged from 1.5 to 2.3 mmol Trolox equivalents (TE) kg −1 and from 0.5 to 0.7 mmol TE kg −1 respectively for blackberries and from 2.9 to 5.2 mmol TE kg −1 and from 0.8 to 1.4 mmol TE kg −1 respectively for blueberries. The HPLC method allowed for complete separation and identification of flavonols commonly found in blackberries, and blueberries. Our results showed that blueberry and blackberry genotypes varied significantly in flavonol content and antioxidant capacity. Even though total flavonol content did not correlate well with antioxidant capacity, their ability to scavenge peroxyl and superoxide anion radicals was apparent.

Chemical quality parameters and bioactive compound content of brazilian berries

“…These results are in agreement with those of Cho et al (2005), who found a correlation coefficient of r 2 = 0.72, when using the oxygen radical-absorbing capacity (DRAC) method, and r 2 = 0.88 when using the photochemiluminescence assay method. These authors reported that several types of phenolics in the fresh extracts varied in their ability to scavenge peroxyl and superoxide anion radicals.…”

“…This agrees with Wang et al (2008), who reported that cyanidin-3-glucoside is the prevalent anthocyanin and responsible for the AC in blackberries. Similarly, six blackberry genotypes analyzed by Cho et al (2005) showed that distribution of cyanidin aglycones in each cultivar ranged from 75 to 84% for cyanidin 3-glucoside, from 1 to 12% for cyanidin 3-rutinoside, from 4 to 8% for cyanidin 3-dioxaloyglucoside, from 3 to 8% for cyanidin 3-dioxaloylglucoside, and from 2 to 3% for cyanidin 3-(malonyl) glucoside. Dther anthocyanins were identified as delfinidin-3-glucoside (between 41.86 and 49.26 mg kg -1 fw), malvinidin-3-glucoside (2.96 to 56.45 mg kg -1 fw).…”

Yaban Mersini (Vaccinium corymbosum L.) Suyu Konsantresinin Püskürtmeli Kurutucuda Kurutulması: Tepki Yüzey Yöntemiyle Optimizasyon

“…Literatürde üzümsü meyve tüketiminin insan sağlığına olumlu katkılarının olduğunu gösteren çalışmalar mevcuttur [2]. Yaban mersini fenolik bileşenlerce, özellikle antosiyaninlerce zengindir [3] ve birçok diğer meyve ve sebzeye göre antioksidan kapasitesi oldukça yüksektir. Fakat onları değerli kılan fenolik bileşenleri genellikle; ısı, ışık, nem, yüksek sıcaklık ve oksijen gibi olumsuz çevresel faktörlere karşı hassas bileşenlerdir.…”

HYDROXYCINNAMIC ACID DERIVATIVES AND FLAVONOL PROFILES OF MAQUI (Aristotelia chilensis) FRUITS

“…In this case, the compound identities were assigned by also considering the fragmentation pattern described by Scoparo 23 , as well as on the basis of the elution order. The first compound eluted (peak 15) was tentatively assigned as quercetin-galloylgalactoside, while the second peak eluted was assigned as quercetin-galloylglucoside (peak 16); both compounds have previously been detected in blueberries 24 . The myricetin derivatives included one rutinoside, one hexoside, one pentoside, one galloylhexoside, and one unidentified derivative.…”