Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity

Kuhn, Benjamin M.; Nodzyński, Tomasz; Errafi, Sanae; Bucher, Rahel; Gupta, Shibu; Aryal, Bibek; Dobrev, Petre I.; Bigler, Laurent; Geisler, Markus; Zažímalová, Eva; Friml, Jiřı́; Ringli, Christoph

doi:10.1038/srep41906

Cited by 52 publications

(45 citation statements)

References 61 publications

(120 reference statements)

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“…The gene encoding for the second enzyme of the flavonoid biosynthesis pathway, chloroplastic chalcone isomerase, is enriched in L1, while the chalcone synthase gene is enriched in D2. It is known that flavonoids affect the expression, localization and recycling of PINs, as well as the activity of ABCB‐type auxin transporters (Brown et al ; Peer & Murphy ; Bailly et al ; Santelia et al ; Kuhn et al ). The formation of local auxin maxima in SAM depends mainly on the action of PINs at L1, and through this layer, auxin arrives at the organ initiation sites (Vernoux et al ).…”

Section: Stress Adaptation Of Apical Meristemsmentioning

confidence: 99%

Redox regulation at the site of primary growth: auxin, cytokinin and ROS crosstalk

Tognetti

Bielach

Hrtyan

2017

Plant Cell & Environment

111

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To maintain the activity of meristems is an absolute requirement for plant growth and development, and the role of the plant hormones auxin and cytokinin in apical meristem function is well established. Only little attention has been given, however, to the function of the reactive oxygen species (ROS) gradient along meristematic tissues and its interplay with hormonal regulatory networks. The interdependency between auxin-related, cytokinin-related and ROS-related circuits controls primary growth and development while modulating plant morphology in response to detrimental environmental factors. Because ROS interaction with redoxactive compounds significantly affects the cellular redox gradient, the latter constitutes an interface for crosstalk between hormone and ROS signalling pathways. This review focuses on the mechanisms underlying ROS-dependent interactions with redox and hormonal components in shoot and root apical meristems which are crucial for meristems maintenance when plants are exposed to environmental hardships. We also emphasize the importance of cell type and the subcellular compartmentalization of ROS and redox networks to obtain a holistic understanding of how apical meristems adapt to stress.

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Section: Stress Adaptation Of Apical Meristemsmentioning

confidence: 99%

Redox regulation at the site of primary growth: auxin, cytokinin and ROS crosstalk

Tognetti

Bielach

Hrtyan

2017

Plant Cell & Environment

111

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“…It has been found that, in Arabidopsis, mutation of the genes encoding cinnamate 4hydroxylase (C4H) and hydroxycinnamoyl-coenzyme A shikimate:quinate hydroxycinnamoyl-transferase (HCT) involved in this pathway resulted in changes in the structural, developmental, and reproductive phenotypes of Arabidopsis plants [31][32][33]. Interestingly, the flavonols quercetin and kaempferol have been shown to inhibit polar auxin transport and to enhance consequent localized auxin accumulation [34][35][36]. Recently, Kuhn, et al [34] suggested that flavonols could modulate auxin transport by modifying the antagonistic kinase/phosphatase equilibrium.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, the flavonols quercetin and kaempferol have been shown to inhibit polar auxin transport and to enhance consequent localized auxin accumulation [34][35][36]. Recently, Kuhn, et al [34] suggested that flavonols could modulate auxin transport by modifying the antagonistic kinase/phosphatase equilibrium. Therefore, flavonols could affect auxin transport in zigzag-shaped shoots.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and metabolite analyses provide insights into zigzag-shaped stem formation in tea plants (Camellia sinensis)

et al. 2020

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Background: Shoot orientation is important for plant architecture formation, and zigzag-shaped shoots are a special trait found in many plants. Zigzag-shaped shoots have been selected and thoroughly studied in Arabidopsis; however, the regulatory mechanism underlying zigzag-shaped shoot development in other plants, especially woody plants, is largely unknown. Results: In this study, tea plants with zigzag-shaped shoots, namely, Qiqu (QQ) and Lianyuanqiqu (LYQQ), were investigated and compared with the erect-shoot tea plant Meizhan (MZ) in an attempt to reveal the regulation of zigzag-shaped shoot formation. Tissue section observation showed that the cell arrangement and shape of zigzagshaped stems were aberrant compared with those of normal shoots. Moreover, a total of 2175 differentially expressed genes (DEGs) were identified from the zigzag-shaped shoots of the tea plants QQ and LYQQ compared to the shoots of MZ using transcriptome sequencing, and the DEGs involved in the "Plant-pathogen interaction", "Phenylpropanoid biosynthesis", "Flavonoid biosynthesis" and "Linoleic acid metabolism" pathways were significantly enriched. Additionally, the DEGs associated with cell expansion, vesicular trafficking, phytohormones, and transcription factors were identified and analysed. Metabolomic analysis showed that 13 metabolites overlapped and were significantly changed in the shoots of QQ and LYQQ compared to MZ. Conclusions: Our results suggest that zigzag-shaped shoot formation might be associated with the gravitropism response and polar auxin transport in tea plants. This study provides a valuable foundation for further understanding the regulation of plant architecture formation and for the cultivation and application of horticultural plants in the future.

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“…It has been found that mutation of the genes encoding the cinnamate 4-hydroxylase (C4H) and hydroxycinnamoyl-coenzyme A shikimate:quinate hydroxycinnamoyl-transferase (HCT) involved in this pathway resulted in changes in the structural, developmental, and reproductive phenotypes of plants [39][40][41]. Interestingly, flavonols, quercetin and kaempferol, have been shown to inhibit polar auxin transport and to enhance consequent localized auxin accumulation [42][43][44]. Recently, Kuhn, et al [42] suggested that flavonols could modulate auxin transport by modifying the antagonistic kinase/phosphatase equilibrium.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, flavonols, quercetin and kaempferol, have been shown to inhibit polar auxin transport and to enhance consequent localized auxin accumulation [42][43][44]. Recently, Kuhn, et al [42] suggested that flavonols could modulate auxin transport by modifying the antagonistic kinase/phosphatase equilibrium. Therefore, flavonols could affect the auxin transport in the zigzag shoot.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and metabolite analyses provide insights into zigzag-stem formation in tea plants (Camellia sinensis)

Cao

Wang²,

Lin

et al. 2020

Preprint

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BackgroundShoot orientation is important for plant architecture formation, and the zigzag shoot is a special trait of many plants. The zigzag-shaped shoot has been selected and well studied in Arabidopsis, however, the regulatory mechanism of zigzag-shaped shoot development in other plants especially woody plants is largely unknown.ResultsIn this study, tea plants with zigzag shoots, namely, Qiqu (QQ) and Lianyuanqiqu (LYQQ), were investigated and compared with the erect-shoot tea plant Meizhan (MZ) in an attempt to reveal the regulation of zigzag-shoot formation. Tissue section observation showed that the cell arrangement and shape of zigzag stems were aberrant compared with those of normal shoots. Moreover, a total of 2175 DEGs were identified from the zigzag shoots of the tea plants QQ and LYQQ compared to the shoots of MZ using transcriptome sequencing, and the DEGs involved in the “Plant-pathogen interaction”, “Phenylpropanoid biosynthesis”, “Flavonoid biosynthesis” and “Linoleic acid metabolism” pathways were significantly enriched. Additionally, the DEGs associated with cell expansion, vesicular trafficking, phytohormones, and transcription factors were identified and analysed. Metabolomic analysis showed that 13 metabolites overlapped and were significantly changed in the shoots of QQ and LYQQ compared to MZ.ConclusionsOur results suggest that zigzag-shaped shoot formation might be associated with the gravitropism response and polar auxin transport in tea plants and provides a valuable foundation for further understanding the regulation of plant architecture formation and for the cultivation and application of horticultural plants in the future.

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Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity

Cited by 52 publications

References 61 publications

Redox regulation at the site of primary growth: auxin, cytokinin and ROS crosstalk

Redox regulation at the site of primary growth: auxin, cytokinin and ROS crosstalk

Transcriptome and metabolite analyses provide insights into zigzag-shaped stem formation in tea plants (Camellia sinensis)

Transcriptome and metabolite analyses provide insights into zigzag-stem formation in tea plants (Camellia sinensis)

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