Flavonols as metal-ion chelators: complex formation with Mg2+ and Ba2+ cations in the excited state

Roshal, Alexander D.; Grigorovich, A. V.; Дорошенко, А. О.; Pivovarenko, Vasyl G.; Demchenko, Alexander P.

doi:10.1016/s1010-6030(99)00105-7

Cited by 85 publications

(68 citation statements)

References 28 publications

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“…7). In addition, flavonols are known to chelate divalent metal cations, and metal chelation leads to the modification of their spectroscopic behavior (Roshal et al, 1999). As a strong flavonol accumulation was observed under L80 + Cd treatment, role of flavonols in Cd chelation can be also postulated.…”

Section: Discussionmentioning

confidence: 99%

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Cd, Fe, and Light Sensitivity: Interrelationships in Cd-Treated Populus

Solti

Gáspár

Vági

et al. 2011

OMICS: A Journal of Integrative Biology

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Cadmium is a toxic heavy metal causing iron deficiency in the shoot and light sensitivity of photosynthetic tissues that leads to decreased photosynthetic performance and biomass production. Light intensity had strong impact on both photosynthetic activity and metal accumulation of cadmium-treated plants. At elevated irradiation, cadmium accumulation increased due to the higher dry mass of plants, but its allocation hardly changed. A considerable amount of iron accumulated in the roots, and iron concentration was higher in leaves developed at moderate rather than low irradiation. At the same time, the higher the irradiation the lower the maximal photochemical quantum efficiency. The decreased photochemical efficiency, however, started to recover after a week of Cd treatment at moderate light without substantial change in metal concentrations but following the accumulation of green fluorescent compounds. Both cadmium treatment and higher light caused the accumulation of flavonoids in leaf mesophyll vacuoles/chloroplasts, but accumulation of flavonols, fluorescing at 510 nm, was characteristic to cadmium stress. Therefore, flavonoids, which may act by scavenging reactive radicals, chelating Cd, and shielding against excess irradiation, play an important part in Cd stress tolerance of Populus, and may have special impact on its phytoremediation capacity.

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Section: Discussionmentioning

confidence: 99%

“…Among flavonoids, flavonols are the main emitters in the green region (Morales et al, 1996;Roshal et al, 1999), whereas flavins perform a higher wavelength, yellow region fluorescence Morales et al, 1994). The synthesis of flavonoids is known to be induced by UV stress (Harborne and Williams, 2000).…”

Section: Discussionmentioning

confidence: 99%

Cd, Fe, and Light Sensitivity: Interrelationships in Cd-Treated Populus

Solti

Gáspár

Vági

et al. 2011

OMICS: A Journal of Integrative Biology

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“…Derivatives of these dyes for covalent labeling of amino and SH-groups were also obtained , which can be used in various types of protein and peptide biosensors. Their azacrown ether derivatives exhibit dramatic spectral responses to the binding of bivalent cations (Roshal et al 1999;1998). Therefore these reporters can provide a link between chemical sensing and biosensing.…”

Section: Supersensitive Multicolor Ratiometric Dyesmentioning

confidence: 99%

Molecular-Size Fluorescence Emitters

Demchenko

2015

Introduction to Fluorescence Sensing

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A variety of fluorescent and luminescent materials in the form of molecules, their complexes and nanoparticles are available for implementation as response units into sensing and imaging technologies and we discuss their general properties that are essential for these applications. Organic dyes were the first and remain to be the most popular emitters used with these purposes. Their labeling and responsive properties are overviewed. Natural fluorescent proteins and their engineered analogues incorporate organic fluorophores that are synthesized spontaneously from the polypeptide chain inside the living cells without the need of their intrusion, as the gene products. They resulted in revolutionary advancement in cell imaging and show good prospects in sensing and reporting on cellular processes. Organic heterocyclic compounds can incorporate metal ions generating long-living luminescence. Moreover, noble metal particles of a very small size exhibit unique light-absorbing and fluorescent emission properties. In this Chapter we focus on these types of fluorophores that possess subnanometer dimensions and display single type of absorption-emission cycle. The more complex nanostructures and nanocomposites will be overviewed in Chaps. 5 and 6 that follow. Fluorophores and Their Characteristics General Properties of Fluorescent DyesIn view of tremendous diversity of structures, chemical reactivities and spectroscopic properties, one needs certain practically useful criteria for the dye selection for the purpose of sensing and imaging. They can be formulated in the form of spectroscopic parameters that have to be optimized. It is the parameter describing the ability of molecule to absorb light at a particular wavelength. The absorbance E measured at any wavelength on spectrophotometer is proportional to the dye concentration c (in mol/l) and the light path length in a sample l (in cm). In this relation, ε plays the role of coefficient of proportionality, so that E = εcl. The value of molar absorbance is obtained by purifying and drying the dye, dissolving it at low concentration and measuring E on a spectrophotometer. The broadly used term extinction includes absorbance and light scattering. Absorbance is a unique characteristic of a molecule under certain environmental conditions. In general, the bigger the fluorophore size, the greater is the probability that the photon will be absorbed.The value of ε max at the maximum of absorption band is the common characteristic of the light-absorbing power of the dye. The dye 'brightness' that determines the absolute sensitivity of fluorescence detection (Wetzl et al. 2003) is the product of molar absorbance and quantum yield, Φ. The absorbance is related to optical cross-section and for organic dyes, ε max varies from ca. 10 3 l mol −1 cm −1 for small dyes, such as coumarins to ca. 10 5 l mol −1 cm −1 for larger fluorophores such as cyanines and phthalocyanines (Lavis and Raines 2008). It should be selected as high as possible, but the limiting factor is the fluorophore size. Us...

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“…Derivatives of these dyes for covalent labeling of amino and SH-groups were also obtained (Klymchenko et al, 2004a), which can be used in various types of protein and peptide biosensors. Their azacrown ether derivatives exhibit dramatic spectral responses to the binding of bivalent cations (Roshal et al, 1998;Roshal et al, 1999). Therefore these reporters can provide a link between chemical sensing and biosensing.…”

Section: Optimized Dyes For Two-band Wavelength-ratiometric Measuremementioning

confidence: 99%

Fluorescence sensing of intermolecular interactions and development of direct molecular biosensors

2006

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Molecular biosensors are devices of molecular size that are designed for sensing different analytes on the basis of biospecific recognition. They should provide two coupled functions - the recognition (specific binding) of the target and the transduction of information about the recognition event into a measurable signal. The present review highlights the achievements and prospects in design and operation of molecular biosensors for which the transduction mechanism is based on fluorescence. We focus on the general strategy of fluorescent molecular sensing, construction of sensor elements, based on natural and designed biopolymers (proteins and nucleic acids). Particular attention is given to the coupling of sensing elements with fluorescent reporter dyes and to the methods for producing efficient fluorescence responses.

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Flavonols as metal-ion chelators: complex formation with Mg2+ and Ba2+ cations in the excited state

Cited by 85 publications

References 28 publications

Cd, Fe, and Light Sensitivity: Interrelationships in Cd-Treated Populus

Cd, Fe, and Light Sensitivity: Interrelationships in Cd-Treated Populus

Molecular-Size Fluorescence Emitters

Fluorescence sensing of intermolecular interactions and development of direct molecular biosensors

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