Flaxseed gum reduces body weight by regulating gut microbiota

Luo, Jianming; Li, Yuetong; Mai, Yunshi; Gao, Lijuan; Ou, Shiyi; Wang, Yong; Liu, Liu; Peng, Xichun

doi:10.1016/j.jff.2018.05.042

Cited by 60 publications

(43 citation statements)

References 26 publications

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“…Dietary polysaccharides (DPs), both insoluble and soluble, can escape the digestion of human digestive enzyme and reach distal colon, and can be further utilized by gut microbiota and benefit human health (Holscher, 2017; Luo, Li, et al., 2018; Luo, Zhang, et al., 2018; Xie et al., 2019). Different DPs with different compositions and structures may lead to various functions.…”

Section: Introductionmentioning

confidence: 99%

Pectin and inulin stimulated the mucus formation at a similar level: An omics‐based comparative analysis

Xie

et al. 2020

Journal of Food Science

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Mucin 2 (MUC2) is the skeleton of colonic mucus that comprises the physical intestinal barrier. Different dietary polysaccharides may affect colonic mucus at different extents. The effect of pectin on MUC2 production is contradictory. To investigate whether and how pectin affected hosts’ colonic mucus, the amount of MUC2 in colon, the cecal, mucosal microbiota, and metabolites profiles were analyzed and compared with inulin. The results showed pectin stimulated the production of MUC2 at a similar level to inulin. Both interventions increased the abundance of cecal Lachnospira and Christensenellaceae_R‐7_group, and enhanced the production of specific metabolites including soyasapogenol B 24‐O‐b‐d‐glucoside, lucyoside Q, trans‐EKODE‐(E)‐Ib, and 1,26‐dicaffeoylhexacosanediol. Additionally, pectin increased the relative abundance (RA) of cecal Lactobacillus, and induced less RA of potentially harmful bacteria such as Helicobacter in mucosal microbiota than inulin. In conclusion, we first reported that pectin and inulin stimulated the mucus formation at a similar level. Two genera of cecal bacteria and four metabolites may play an important role in enhancing the production of MUC2. Moreover, the MUC2 production may be unrelated to several traditional health‐beneficial bacteria; pectin possibly performed as good as or better than the inulin in rats’ gut.

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Section: Introductionmentioning

confidence: 99%

Pectin and inulin stimulated the mucus formation at a similar level: An omics‐based comparative analysis

Xie

et al. 2020

Journal of Food Science

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“…In recent years, studies on the mechanisms of the beneficial effects of functional foods have attracted lots of interests (Cabrera, Artacho, & Giménez, ; Huang et al., ; Luo et al., , ; Peng et al., ; Schroeter et al., ; Shahidi, & Ambigaipalan, ). However, reports on the beneficial effects of alternate consumption of bioactive components in traditional Asian diet according to the profile of gut microbes and colonic immunity are rather limited.…”

Section: Discussionmentioning

confidence: 99%

“…The processing and bioinformatics analysis of the 16S rDNA gene sequencing data are performed as described previously (Huang et al., ; Luo et al., ).…”

Section: Methodsmentioning

confidence: 99%

The Alternate Consumption of Quercetin and Alliin in the Traditional Asian Diet Reshaped Microbiota and Altered Gene Expression of Colonic Epithelial Cells in Rats

Guo

Xie

et al. 2019

Journal of Food Science

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The diet of traditional Asian is similar to the Mediterranean that was considered as a healthy dietary pattern. The report was scarce on whether different plant‐derived components with similar anti‐oxidative and anti‐inflammatory function such as quercetin and alliin in traditional Asian diet consumed in an alternate style cooperatively affect health including the growth of host and the status of the gut microbiota and colonic epithelial immunity. In the present study, the effects of alternate consumption of quercetin and alliin on host health judging by the profile of gut microbiota and gene expression of colonic epithelial cells were investigated with the Illumina MiSeq sequencing (16S rRNA genes) and Illumina HiSeq (RNA‐seq) technique, respectively. The results showed that the alternate consumption significantly increased the rat body weight and reshaped the gut microbiota composition. At the phylum level, it significantly increased the relative abundance of fecal Firmicutes and Cyanobacteria but decreased that of Bacteroidetes (P < 0.05) and increased the relative abundance of Candidatus Arthromitus, Lactococcus, Geobacillus, and Ruminococcus at the genus level that benefits the host's health. The alternate consumption of quercetin and alliin also altered 13 genes expression involved in the KEGG pathways of complement and coagulation cascades and hematopoietic cell lineage to improve the gut immunity. Therefore, the alternate consumption of quercetin and alliin in traditional Asian diet can contribute beneficial metabolic effects by optimizing gut microbiota and altering the immunologic function of colonic epithelial cells, resulting in its potential to improve the sub‐health status.

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Section: Methodsmentioning

confidence: 99%

“…The PCR was run with a 20 μl reaction system containing 4 μl of 5 × FastPfu buffer, 2 μl of 2.5 mM dNTPs, 0.8 μl of each primer (5 μM), 0.4 μl of FastPfu polymerase, and 10 ng of template DNA. The thermocycler PCR system was used to perform the PCR (ABI GeneAmp ® 9700) using the following program: denaturation at 95°C for 3 min, then 27 cycles of amplification including denaturation at 95°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 45 s, with a final extension at 72°C for 10 min(Luo, Li, et al, 2018b).Amplicons were extracted from 2% agarose gels, purified with the AxyPrep DNA gel extraction kit according to the manufacturer's instructions, and quantified using QuantiFluor™ -ST. According to the standard protocols, the purified amplicons were pooled in equimolar amounts and paired-end sequenced (2 × 250) on an Illumina MiSeq platform.…”

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confidence: 99%

Alternating consumption of β‐glucan and quercetin reduces mortality in mice with colorectal cancer

et al. 2019

Food Science & Nutrition

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The current dietary recommendations for disease prevention and management are scarce and are not well supported. Beta‐glucan or quercetin in a diet can alleviate colorectal cancer (CRC) by regulating the gut microbiota and related genes, but the effects of alternating their consumption for routine ingestion during CRC occurrence remain unknown. This study investigated the effects of alternating the consumption of β‐glucan and quercetin for routine ingestion on CRC development in mice. The mortality rate, colonic length, inflammatory cytokines, gut microbiota, and colonic epithelial gene expression in healthy and CRC mice that consumed normal and alternate diets were compared and studied. The results showed that alternating the consumption of β‐glucan and quercetin (alternating among a β‐glucan diet, a normal diet and a normal diet that was supplemented with quercetin) alleviated colon damage and reduced the mortality rate in CRC mice, with a reduction in mortality of 12.5%. Alternating the consumption of β‐glucan and quercetin significantly decreased the TNF‐α level, increased the relative abundance of Parabacteroides, and downregulated three genes (Hmgcs2, Fabp2, and Gpt) that are associated with inflammation and cancer. Alternating the consumption of some bioactive compounds, such as β‐glucan and quercetin, in food can contribute to human health. This experiment provided some experimental evidence for the dietary recommendations for disease prevention and management.

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Flaxseed gum reduces body weight by regulating gut microbiota

Cited by 60 publications

References 26 publications

Pectin and inulin stimulated the mucus formation at a similar level: An omics‐based comparative analysis

Pectin and inulin stimulated the mucus formation at a similar level: An omics‐based comparative analysis

The Alternate Consumption of Quercetin and Alliin in the Traditional Asian Diet Reshaped Microbiota and Altered Gene Expression of Colonic Epithelial Cells in Rats

Alternating consumption of β‐glucan and quercetin reduces mortality in mice with colorectal cancer

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