The current research aims to validate the effect of flaxseed lignan (secoisolariciresinol diglucoside (SDG)) enriched dahi (analogues to yoghurt) in preventing menopausal osteoporosis in-vivo using an ovariectomized (OVX) rat model. Forty female Albino Wistar rats (3 months old, > 180 g) were divided into sham, OVX control, OVX + control dahi, OVX + SDG enriched dahi groups (n = 8). The results of the animal study indicated the serum calcium and estrogen levels and the bone ash calcium were decreased by 32.27%, 30.95% and 48.46% respectively after ovariectomy, compared to the sham group. The administration of the control dahi increased the serum calcium and estrogen levels as well as the bone ash calcium by 6.26%, 38.62% and 44.57%, while, the daily administration of the SDG enriched dahi (20 g, ~ 260 mg SDG) for eight weeks increased them by 37.37%, 85.52% and 82.34%, respectively, compared to OVX group. The micro-CT analysis of proximal tibial metaphysis region revealed that the ovariectomy decreased the bone mineral density (BMD) and trabecular thickness (Tb.Th) by 11.06% and 90.69%, while the trabecular separation (Tb.Sp.) was increased by 90.69% compared to the sham control group rats. The administration of the control dahi increased the BMD and Tb.Th by 8.85% and 10.04%, and decreased Tb.Sp by 22.13%, whereas the supplementation of SDG enriched dahi increased them by 13.84% and 26.10%, respectively and decreased the Tb.Sp by 88.82% compared to OVX rats. Similar results were obtained in the micro-CT analysis of distal femur epiphysis region. Hence, based on the above results, it could be concluded that the developed product could be useful in alleviating postmenopausal osteoporosis.