Flaxseed mucilage/calcium phosphate composites as bioactive material for bone tissue regeneration

Abstract

Biocompatible polymers are attractive material for the manufacturing of surgical implants which break down in vivo without the necessity for a consequent operation for removal. Elaboration of composite biomaterials scaffolds as artificial bone graft materials remains a major task in bioengineering. Flaxseed mucilage was used as bioactive polysaccharide for preparing composite scaffolds made of calcium phosphate embedded in mucilage matrix. Calcium chloride was mixed with mucilage followed by the addition of phosphate precursor to stimulate the in situ formation of calcium phosphate. The obtained scaffolds mucilage/calcium phosphate at different pHs (5 and 8) were characterized using FTIR, XRD, TGA, SEM/EDX and TEM. The results showed the formation of two phases: mucilage/dicalcium phosphate dihydrate (MU/brushite) and mucilage/hydroxyapatite (MU/HA). MTT test was applied to evaluate viability of MC3T3-E1 osteoblasts cells, and the formed hybrids at various pH conditions were classified as non-cytotoxic. These findings establish the potential of developed composite to be used as bone graft substitute materials.