Flea allergy dermatitis in the cat: Establishment of a functional in vitro test

Stuke, Kristin; Samson‐Himmelstjerna, Georg von; Mencke, Norbert; Hansen, O.; Schnieder, Thomas; Leibold, W.

doi:10.1007/s00436-003-0914-6

Cited by 13 publications

(9 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some showed that plasma from dogs or cats with a history of atopy or food allergy can induce histamine release from human basophils in vitro [ 104 , 105 , 106 ]. Others used the patient’s basophils themselves, as done in human medicine [ 73 , 107 ]. While this review focuses on small animal medicine, the readers should be aware that the BAT has also been investigated for its research and clinical applications in horses with insect bite allergy (“summer eczema”) [ 108 , 109 , 110 , 111 ].…”

Section: Cellular In Vitro Testsmentioning

confidence: 99%

In Vitro Research Tools in the Field of Human Immediate Drug Hypersensitivity and Their Present Use in Small Animal Veterinary Medicine

Lavergne

2016

Veterinary Sciences

View full text Add to dashboard Cite

Drug hypersensitivity reactions (DHR) are immune-mediated idiosyncratic adverse drug events. Type I DHR are often referred to as “immediate” and involve B lymphocyte-secreted IgE that bind to the membrane of basophils and mast cells, inducing their degranulation. This review presents various in vitro tests that were developed in the field of human type I HS and implemented as clinical diagnostic tools in human cases of immediate DHR. The respective strengths and weaknesses of each test will be discussed in parallel of validation data such as specificity and sensitivity whenever available. Some of them have also been used as diagnostic tools in veterinary medicine, but not in cases of immediate DHR. Most of these diagnostic tools can be categorized into humoral and cellular tests. The former tests measure serum concentrations of factors, such as histamine, tryptase, and drug-specific IgE. The latter assays quantify markers of drug-induced basophil activation or drug-specific lymphocyte proliferation. Pharmacogenetic markers have also been investigated in immediate DHR, but not as extensively as in non-immediate ones. Throughout, practical aspects and limitations of the tests, as well as sensitivity and specificity parameters, will be presented. In addition, the experience of veterinary medicine with these diagnostic tools will be summarized. However, to date, none of them has ever been reported in a veterinary case of type I DHR.

show abstract

Section: Cellular In Vitro Testsmentioning

confidence: 99%

In Vitro Research Tools in the Field of Human Immediate Drug Hypersensitivity and Their Present Use in Small Animal Veterinary Medicine

Lavergne

2016

Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…columella populations to F . hepatica infection were primarily based on the presence or absence of a characteristic band of small sharp spots in the mid-region of the mantle, as its occurrence is associated with the resistant phenotype 19 . Additionally, both phenotypes, and particularly that of the resistant populations, were double-checked in the laboratory by experimental infection using different panels of potentially sympatric (retrieved from infected cattle reared at the site or in nearby areas) and allopatric F .…”

Section: Methodsmentioning

confidence: 99%

“…columella in recent decades due to the existence of a resistant phenotype against F . hepatica infection in certain natural Cuban populations 19,20 . Experimental exposures of these resistant snails to different sympatric and allopatric fluke isolates always result in the failure of parasite larval stage development 19–22 which is associated with an effective encapsulation of the parasite by snail immune cells 19 .…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, thus far, all resistant P . columella can be easily differentiated from susceptible individuals in the field though the use of a phenotypic marker consisting of a belt-like pattern of small, sharp and whitish spots in the mid-region of the mantle with bigger spots scattered on the upper and lower sides 19 . Attempts to genetically differentiate susceptible from resistant individuals have independently clustered both phenotypes by RAPD markers 21 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Patterns of distribution, population genetics and ecological requirements of field-occurring resistant and susceptible Pseudosuccinea columella snails to Fasciola hepatica in Cuba

Alba

Vázquez

Sánchez

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Pseudosuccinea columella snails transmit the trematode Fasciola hepatica, but in Cuba, six naturally occurring populations successfully resist parasite infection. Here, we present an updated distribution of P. columella in Cuba; 68 positive sites with the earliest records more abundant in west-central Cuba and with east-central populations generally corresponding to the newest samples. No records were found farther east. The IPA site reported 10.5% prevalence of F. hepatica-infected snails. Population genetics, studied through microsatellites, showed low allelic and multilocus genotypic richness (MLGT), mainly in susceptible populations, strong deviations from panmixia and high self-fertilization rates. Susceptible individuals were grouped in one major cluster containing the majority of MLGT, and two independent clusters grouped the MLGT of resistant individuals from western and central populations, respectively. From these, we propose that several introductions of P. columella occurred in Cuba, primarily in the west, with the early arrivals deriving on the resistant populations. A more recent introduction of susceptible P. columella carrying MLGT T and Y may have occurred, where the latter spread quickly through the island and possibly increase the risk of parasite transmission in Cuba since all snails naturally infected with F. hepatica were carriers of the MLGT Y. Interestingly, even though resistant populations are highly diverse and are likely the oldest within Cuba, they are only found in six localities characterized by soft (total hardness, TH = 6.3 ± 1.03°d) and slightly acidic (pH = 6.2 ± 0.12) waters with low richness in snail species (3.2 ± 1.02). This tendency was also observed in a two-year follow-up ecological study that was conducted on a farm where both phenotypes occurred in sympatry; colonization events by resistant over susceptible snails coincided with a reduction in the pH and TH of the water. A comparison of life traits in susceptible and resistant isolates reared at two different pH/TH conditions (5.9/4°d or 7.8/14°d) showed that low pH/TH negatively affects P. columella, irrespective of the phenotype. However, evidence of higher tolerance (higher survival, life expectancy, egg viability) to such conditions was observed in resistant isolates. Finally, we speculate that the limited distribution of resistant populations might be related to a better exploitation of sites that are less suitable to snails (thus, with lower competition), rather than to a differential ecological restriction to specific environmental conditions from susceptible P. columella.

show abstract

“…The functional in vitro test for sensitized type I allergic effector cells (FIT)-basically developed for horses by Kaul (1998)provides a new in vitro monitoring system for feline allergen-specific antibodies that were bound in vivo to sensitize effector cells of type I allergies such as basophilic granulocytes and mast cells (Stuke et al 2003). To test the existence of allergen-specific, sensitizing antibodies on basophils with the FIT, washed blood cells are incubated in vitro with selected antigen.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of basophil sensitization to antigens of the cat flea (Ctenocephalides felis felis): a new tool for the diagnosis of feline flea bite hypersensitivity?

et al. 2008

Self Cite

View full text Add to dashboard Cite

Suitability of blood basophils for in vitro diagnosis of flea allergy dermatitis (FAD) or flea bite hypersensitivity was studied in cats. A functional in vitro test (FIT) for sensitized type I allergic effector cells was used to evaluate the degree and kinetics of in vivo basophil sensitization against flea antigens in cats under long-term flea exposure. FIT results were compared with intradermal (IDT) and serological testing. Before, during, and after weekly repeated exposure to Ctenocephalides felis; 14 cats were repetitively FIT-assessed for general and flea-specific sensitization. In three cats, flea-specific sensitization was seen before and throughout flea exposure. Five cats, although generally sensitized, never developed a flea-specific sensitization. Six cats initially FIT-negative became sensitized for flea antigen during flea infestation. Induction, upregulation, and binding of C. felis-specific sensitizing antibodies to basophils during flea challenge may explain the developing sensitization in these cats. Strong discrepancies between the levels of flea-specific circulating IgE and basophil sensitization contrasted comparable results for basophil and mast cell sensitization using FIT and IDT, respectively. Hence, the FIT might provide an immunological supplement to the clinical diagnosis of FAD in cats by elucidating the state of basophil-sensitization to flea antigens. And it may be a comfortable alternative to IDT.

show abstract

Flea allergy dermatitis in the cat: Establishment of a functional in vitro test

Cited by 13 publications

References 4 publications

In Vitro Research Tools in the Field of Human Immediate Drug Hypersensitivity and Their Present Use in Small Animal Veterinary Medicine

In Vitro Research Tools in the Field of Human Immediate Drug Hypersensitivity and Their Present Use in Small Animal Veterinary Medicine

Patterns of distribution, population genetics and ecological requirements of field-occurring resistant and susceptible Pseudosuccinea columella snails to Fasciola hepatica in Cuba

Monitoring of basophil sensitization to antigens of the cat flea (Ctenocephalides felis felis): a new tool for the diagnosis of feline flea bite hypersensitivity?

Contact Info

Product

Resources

About