Abstract. Two new nematode species, Philometra strongylurae sp. n. and Philometra tylosuri sp. n., are described from female specimens collected from needlefishes (Belonidae, Beloniformes) off the Fao coast, Basrah, Persian Gulf, in Iraq. Philometra strongylurae (gravid females with larvae) was recorded from the subcutaneous tissue, muscles of beak and gills of Strongylura leiura (type host) and S. strongylura, whereas P. tylosuri (nongravid females) from the musculature and the subcutaneous tissue of Tylosurus crocodilus. The former is characterized mainly by the presence of eight markedly large cephalic papillae, conspicuously large anterior oesophageal bulb and by the length of body (15-20 mm); the latter by eight minute cephalic papillae, poorly developed oesophageal bulb and by the body length (46-72 mm). A key to Philometra spp. with gravid females parasitic in the subcutaneous tissue, fins or musculature of fishes is provided.Parasitological examinations of three species of marine fishes, the banded needlefish Strongylura leiura (Bleeker, 1850), the spottail needlefish Strongylura strongylura (van Hasselt, 1823)
MATERIALS AND METHODSFishes were deep-frozen after capture and were dissected in the laboratory; the skin was removed to reveal the parasites. Nematodes recovered were washed in physiological saline, fixed in Berland's fluid (19 parts pure glacial acid, 1 part 40% formaldehyde solution), and stored in 70% ethanol. For light microscopical examination, they were cleared with glycerine. Drawings were prepared with the aid of a Zeiss microscope drawing attachment. After examination, specimens were stored in 70% ethanol. Two specimens of each species (complete specimens or body fragments) were also used for scanning electron microscopy. Nematodes were transferred to 4% formaldehyde solution and postfixed in 1% osmium tetroxide, dehydrated through a graded ethanol series, critical point dried and sputter-coated with gold; they were examined using a JEOL JSM-6300 scanning electron microscope at an accelerating voltage of 15 kV. All measurements are in micrometres unless otherwise stated. Scientific names of fishes follow Froese and Pauly (2004). Since many specimens of Philometra recovered from needlefishes were unidentifiable to species because of their poor condition and because more members of Philometra may occur in the same fish host species, data could not be interpreted to provide prevalence and intensity of infection.
RESULTSPhilometra strongylurae sp. n.
Figs. 1, 2Description of gravid female (4 specimens; measurements of holotype in parentheses; measurements of 2 additional subgravid females in square brackets): Body of live specimens red, that of fixed specimens greyish, with distinctly visible light-brown intestine. Body cylindrical,] mm long, somewhat tapering at both ends; posterior part somewhat narrower than anterior part; maximum width of body 666-925 (884) [571 and 625] at region of oesophagus, width of cephalic end 204-299 (286) [231 and 258], of caudal end 204-231 (204) [19...