Manipulating the optical polarization states has been demonstrated to be effective for communications, signal processing, and sensing. As a unique two-dimensional material, oriented carbon nanotube (CNT) films possess a strong polarization dependence effect in ultra-wide optical spectrum and fascinating physical properties. Furthermore, its remarkable compatibility with the micro-nano fabrication process enables its great potential in the integration with microelectromechanical (MEMS) devices. However, the polarization performance of CNT film is sensitive to many factors, which makes the integration with devices challenging when precise locations and angles are required. We address this issue by assembling CNTs into a tightly packed and aligned film from which the polarization properties were explored related to film thickness, tube alignment, and internal impurity, etc. The mechanisms of how the CNT film polarization properties were determined by such factors were analyzed. Some practically effective methods to enhance its polarization capability were proposed simultaneously. More specifically, a sample possessing a degree of polarization (DOP) up to 99% and transmittance over 45% was obtained through proper regulations. The validated optimization makes the aligned CNT films more feasible and valuable for the integration of the CNT polarizers with MEMS technology.