Flexible elastomer patch with vertical silicon nanoneedles for intracellular and intratissue nanoinjection of biomolecules

Jang, Hanmin; Kim, Bongjoong; Kim, Min Ku; Wie, Dae Seung; Lee, Heung Soo; Kim, Dong Rip; Lee, Chi Hwan

doi:10.1126/sciadv.aau6972

Cited by 43 publications

(52 citation statements)

References 33 publications

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“…1A ). During this peeling process, mechanical stresses were intensively concentrated at the bottom undercuts of Si NNs to generate cracks, leading to their physical separation from the donor Si wafer ( 16 ). The scanning electron microscopy (SEM) images (bottom) highlight the uniformly cracked Si NNs at the bottom undercuts with the exposed length of ~15 μm.…”

Section: Resultsmentioning

confidence: 99%

Biodegradable silicon nanoneedles for ocular drug delivery

et al. 2022

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Ocular drug delivery remains a grand challenge due to the complex structure of the eye. Here, we introduce a unique platform of ocular drug delivery through the integration of silicon nanoneedles with a tear-soluble contact lens. The silicon nanoneedles can penetrate into the cornea in a minimally invasive manner and then undergo gradual degradation over the course of months, enabling painless and long-term sustained delivery of ocular drugs. The tear-soluble contact lens can fit a variety of corneal sizes and then quickly dissolve in tear fluid within a minute, enabling an initial burst release of anti-inflammatory drugs. We demonstrated the utility of this platform in effectively treating a chronic ocular disease, such as corneal neovascularization, in a rabbit model without showing a notable side effect over current standard therapies. This platform could also be useful in treating other chronic ocular diseases.

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Section: Resultsmentioning

confidence: 99%

Biodegradable silicon nanoneedles for ocular drug delivery

et al. 2022

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“…7 (a)). Over the past decade, extensive biological studies have been performed using Si nanowires [ [123] , [124] , [125] , [126] , [127] , [128] ] using a wide range of cells. Biodegradable nanoneedles were also fabricated by Chiappini et al.…”

Section: Microfluidic Mechanoporationmentioning

confidence: 99%

Microfluidic mechanoporation for cellular delivery and analysis

Chakrabarty

Gupta

Illath

et al. 2022

Materials Today Bio

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Highly efficient intracellular delivery strategies are essential for developing therapeutic, diagnostic, biological, and various biomedical applications. The recent advancement of micro/nanotechnology has focused numerous researches towards developing microfluidic device-based strategies due to the associated high throughput delivery, cost-effectiveness, robustness, and biocompatible nature. The delivery strategies can be carrier-mediated or membrane disruption-based, where membrane disruption methods find popularity due to reduced toxicity, enhanced delivery efficiency, and cell viability. Among all of the membrane disruption techniques, the mechanoporation strategies are advantageous because of no external energy source required for membrane deformation, thereby achieving high delivery efficiencies and increased cell viability into different cell types with negligible toxicity. The past two decades have consequently seen a tremendous boost in mechanoporation-based research for intracellular delivery and cellular analysis. This article provides a brief review of the most recent developments on microfluidic-based mechanoporation strategies such as microinjection, nanoneedle arrays, cell-squeezing, and hydroporation techniques with their working principle, device fabrication, cellular delivery, and analysis. Moreover, a brief discussion of the different mechanoporation strategies integrated with other delivery methods has also been provided. Finally, the advantages, limitations, and future prospects of this technique are discussed compared to other intracellular delivery techniques.

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“…This advantage has been demonstrated with the use of nanowires to transfect primary immune cells (Figure a) and the nanostraw electroporation platform that successfully transfected primary cells such as stem cells, cardiomyocytes, and neurons (Figure b) . Recently, Kim and colleagues also developed a vertically ordered silicon-based nanoneedle patch to deliver biomolecules to skin and muscle cell tissues . A key innovation of this method is the use of the elastomer polydimethylsiloxane (PDMS) to coat the nanoneedles, which provides mechanical flexibility and optical transparency (Figure c).…”

Section: Goals Of Cell Transfectionmentioning

confidence: 99%

The Benefits of Going Small: Nanostructures for Mammalian Cell Transfection

Tay

2020

ACS Nano

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Nanostructures, with their localized interactions with mammalian cells, can offer better efficiency and lower cell perturbation than conventional viral, biochemical, and electroporation transfection techniques. In this Perspective, I describe the different stages of transfection and provide a comparison of transfection techniques based on their mechanisms. Focusing on specific aims of transfection, I also illustrate how recent developments in high-aspect-ratio nanostructures have endowed them with properties that are superior to existing viral, biochemical, and electroporation methods as a versatile technique to deliver a variety of cargoes and to interface with different mammalian cell types for biomedical applications. Finally, I describe the challenges associated with transfection that need to be overcome to enhance cargo delivery efficiency and clinical translation.

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Flexible elastomer patch with vertical silicon nanoneedles for intracellular and intratissue nanoinjection of biomolecules

Abstract: A thin transparent wearable patch with silicon nanoneedles is developed for efficient interface with biological cells and tissues.

Cited by 43 publications

References 33 publications

Biodegradable silicon nanoneedles for ocular drug delivery

Biodegradable silicon nanoneedles for ocular drug delivery

Microfluidic mechanoporation for cellular delivery and analysis

The Benefits of Going Small: Nanostructures for Mammalian Cell Transfection

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