“…40,41 The development achieved in this area along with new detection systems using colorimetry to electrochemical, chemiluminescence, electrochemiluminescence, fluorescence, and electrical methods has opened avenues for new sensing technologies. 12,34,[42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57] Paper-based microfluidic systems, however, suffer from two major drawbacks: first is the leakage of sample from the rear side of the channels during sample analysis and second is the difficulty in precise incorporation of reagents in the test zone without the inherent spreading of reagents to the surrounding areas including the microchannels. Additionally, covalent immobilization of certain detection reagents (e.g., antibodies, aptamers, enzymes, etc.)…”