The factors influencing the accuracy of the line scan imaging system (LSIS) are highly complex, and their error propagation mechanisms are poorly clarified. To reasonably and effectively mitigate the impact of mechanical factors on imaging accuracy, a novel comprehensive error model for LSIS is proposed in this paper, revealing the relationship between imaging errors and various mechanical error sources from manufacturing, assembly, and movement. Based on the analysis of the generation and propagation relationship of mechanical errors in LSIS, the integration of the mechanical system and the imaging system is accomplished to form a comprehensive multibody system, thus establishing a complete linear transmission mechanism for mechanical errors in the imaging process within the model. The experiments demonstrate that this model can provide a reference for the localization of mechanical error sources by utilizing the changes in extrinsic parameters during the calibration process. Furthermore, when the sensitivities of error sources are not exceptionally low, and certain cases with systematic errors are excluded, the model can make accurate estimations of the sensitivities of imaging errors to those errors generated by various mechanical sources. The estimated relative errors do not exceed 6%. The results confirm the effectiveness of this model, which can be utilized to ascertain the range of error sources and assess the extent to which errors from various sources impact imaging accuracy. This model provides a basis for the precision optimization of LSIS.