2024
DOI: 10.1038/s41467-024-47697-4
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Flexible TAM requirement of TnpB enables efficient single-nucleotide editing with expanded targeting scope

Xu Feng,
Ruyi Xu,
Jianglan Liao
et al.

Abstract: TnpBs encoded by the IS200/IS605 family transposon are among the most abundant prokaryotic proteins from which type V CRISPR-Cas nucleases may have evolved. Since bacterial TnpBs can be programmed for RNA-guided dsDNA cleavage in the presence of a transposon-adjacent motif (TAM), these nucleases hold immense promise for genome editing. However, the activity and targeting specificity of TnpB in homology-directed gene editing remain unknown. Here we report that a thermophilic archaeal TnpB enables efficient gene… Show more

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