Flies as Forensic Indicators

Greenberg, Bernard

doi:10.1093/jmedent/28.5.565

Cited by 534 publications

(436 citation statements)

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“…Studies of the biological cycle of L. sericata have been conducted previously on samples taken from decaying corpses to determine the PMI (Kamal, 1958;Greenberg, 1991;Anderson, 2000;Grassberger & Reiter, 2001), and some works with this species have dealt with age structure, mortality rates and reproductive output in populations in northeast Europe using samples developed under field conditions (Wall, 1993;Smith & Wall, 1998;Hayes et al, 1999;Pitts & Wall, 2004). However, there has been less research on life tables and reproductive and population parameters carried out under laboratory conditions with samples of the species from a neotropical country like Colombia, in order to analyze average data from several consecutive generations, as was done in the present study.…”

Section: Introductionmentioning

confidence: 99%

Lucilia sericata strain from Colombia: Experimental Colonization, Life Tables and Evaluation of Two Artifcial Diets of the Blowfy Lucilia sericata (Meigen) (Diptera: Calliphoridae), Bogotá, Colombia Strain

et al. 2010

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The objective of this work was to establish, under experimental laboratory conditions, a colony of Lucilia sericata, Bogotá-Colombia strain, to build life tables and evaluate two artificial diets. This blowfly is frequently used in postmortem interval studies and in injury treatment. The parental adult insects collected in Bogotá were maintained in cages at 22°C±1 average temperature, 60%±5 relative humidity and 12 h photoperiodicity. The blowflies were fed on two artificial diets that were evaluated over seven continuous generations. Reproductive and population parameters were assessed. The life cycle of the species was expressed in the number of days of the different stages: egg = 0.8±0.1, larvae I = 1.1±0.02, larvae II = 1.94±0.16, larvae III = 3.5±0.54, pupae = 6.55±0.47, male adult = 28.7±0.83 and female adult = 33.5±1.0. Total survival from egg stage to adult stage was 91.2% for diet 1, while for diet 2 this parameter was 40.5%. The lifetime reproductive output was 184.51±11.2 eggs per female. The population parameters, as well as the reproductive output of the blowflies that were assessed, showed relatively high values, giving evidence of the continuous increase of the strain over the different generations and making possible its maintenance as a stable colony that has lasted for more than two years.

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Section: Introductionmentioning

confidence: 99%

Lucilia sericata strain from Colombia: Experimental Colonization, Life Tables and Evaluation of Two Artifcial Diets of the Blowfy Lucilia sericata (Meigen) (Diptera: Calliphoridae), Bogotá, Colombia Strain

et al. 2010

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“…Proper species identification can be made by a competent entomologist, and rates of development from egg to third instar under prescribed temperatures are know for certain calliphorid flies (Kamal 1958;Greenberg 1991), thus an experienced forensic entomologist can calculate a reasonable PMI estimate. One potential source of error, however, is a reliable estimate of the time of oviposition; i.e., the "starting time" for egg laying and subsequent larval development.…”

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confidence: 99%

Behavioral Patterns of Adult Carrion Flies Phormiaregina and Luciliacoeruleiviridis (Diptera: Calliphoridae) at Pig Carcasses in West Virginia, USA.

Joy¹,

LeGrow²

2015

AIJB

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A study to determine adult arrival behaviors of two forensically important carrion flies (Diptera: Calliphoridae), Phormiaregina (Meigen) and Luciliacoeruleiviridis (Macquart), was conducted at a wildlife management area in southwestern West arrive at carcasses in warm experimental periods within minutes of carcass placement (i.e., at 0 h). At carcasses in cool periods, however, arrival is delayed by 24 h for L. coeruleiviridis at both sunlit and shaded carcasses and by 30 h to 48 h for P. Regina at sunlit and shaded carcasses, respectively. Phormiaregina adults are significantly predisposed to visiting sunlit carcasses, whereas L. coeruleiviridis adults are significantly more likely to visit shaded carcasses.

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“…However, to determine the age 25 of larvae can be challenging and often requires a highly knowledgeable forensic entomologist. The 26 post-feeding larvae also remain to be a problematic stage to age due to the lack of morphological 27 changes occurring with time [5] and therefore the analyst must rely heavily on growth charts. DNA- 28 based analyses have been applied to the field of forensic entomology for over a decade [6].…”

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confidence: 99%

Age estimation of Calliphora (Diptera: Calliphoridae) larvae using cuticular hydrocarbon analysis and Artificial Neural Networks

Moore

Butcher

Adam

et al. 2016

Forensic Science International

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Cuticular hydrocarbons were extracted daily from the larvae of two closely related blowflies Calliphora vicina and Calliphora vomitoria (Diptera:Calliphoridae). The hydrocarbons were then analysed using Gas Chromatography-Mass Spectrometry (GC-MS), with the aim of observing changes within their chemical profiles in order to determine the larval age. The hydrocarbons were examined daily for each species from 1 day old larvae until pupariation. The results show significant chemical changes occurring from the younger larvae to the post-feeding larvae. With the aid of a multivariate statistical method (Principal Component Analysis and Artificial Neural Networks), samples were clustered and classified, allowing for the larval age to be established. Results from this study allowed larvae to be aged to the day with at worst, 87% accuracy, which suggests there is great potential for the use of cuticular hydrocarbons present on larvae to give an indication of their age and hence potentially a valuable tool for minimum PMI estimations.

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Flies as Forensic Indicators

Cited by 534 publications

References 0 publications

Lucilia sericata strain from Colombia: Experimental Colonization, Life Tables and Evaluation of Two Artifcial Diets of the Blowfy Lucilia sericata (Meigen) (Diptera: Calliphoridae), Bogotá, Colombia Strain

Lucilia sericata strain from Colombia: Experimental Colonization, Life Tables and Evaluation of Two Artifcial Diets of the Blowfy Lucilia sericata (Meigen) (Diptera: Calliphoridae), Bogotá, Colombia Strain

Behavioral Patterns of Adult Carrion Flies Phormiaregina and Luciliacoeruleiviridis (Diptera: Calliphoridae) at Pig Carcasses in West Virginia, USA.

Age estimation of Calliphora (Diptera: Calliphoridae) larvae using cuticular hydrocarbon analysis and Artificial Neural Networks

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