FLO5 gene controls flocculation phenotype and adhesive properties in a Saccharomyces cerevisiae sparkling wine strain

Gianvito, Paola Di; Tesnière, Catherine; Suzzi, Giovanna; Blondin, Bruno; Tofalo, Rosanna

doi:10.1038/s41598-017-09990-9

Cited by 36 publications

(20 citation statements)

References 64 publications

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“…Cheats could therefore reap the benefits of flocculating without paying the cost of expressing FLO1 [39]. In fact, there is evidence that lossof-function mutants can occur in and spread through natural populations of yeast expressing the FLO1 homologue, FLO5 [40]. This provides support for the prediction that cheating mutants can only spread in multicellular groups that have formed through aggregation, and not through clonal development.…”

Section: Why Is Group Formation Important?mentioning

confidence: 89%

Multicellular Group Formation in <em>Saccharomyces cerevisiae</em>

Fisher

Regenberg

2018

Preprint

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Understanding how and why cells cooperate to form multicellular organisms is a central aim of evolutionary biology. Multicellular groups can form through clonal development (where daughter cells stick to mother cells after division) or by aggregation (where cells aggregate to form groups). These different ways of forming groups directly affect relatedness between individual cells, which in turn influences the degree of cooperation and conflict within the multicellular group. It is hard to study the factors that favoured multicellularity by focusing only on obligately multicellular organisms, like complex animals and plants, because the factors that favour multicellular cooperation cannot be disentangled, as cells cannot survive and reproduce independently. We propose bakers yeast, Saccharomyces cerevisiae, as an ideal model for studying the very first stages of the evolution of multicellularity. This is because it can form multicellular groups both clonally and through aggregation and uses a family of proteins called ‘flocculins’ that determine the way in which groups form, making it particularly amenable to lab experiments. We briefly review current knowledge about multicellularity in S. cerevisiae and then propose a framework for making predictions about the evolution of multicellular phenotypes in yeast based on social evolution theory. We finish by suggesting outstanding questions and potentially fruitful avenues for future research.

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Section: Why Is Group Formation Important?mentioning

confidence: 89%

Multicellular Group Formation in <em>Saccharomyces cerevisiae</em>

Fisher

Regenberg

2018

Preprint

View full text Add to dashboard Cite

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“…A more recent study with a high flocculant S. cerevisiae strain showed that FLO5 plays an important role in cell-surface adhesion. In fact, the deletion of FLO5 caused a strong reduction in flocculation capacity when compared with the deletion of FLO1 [64]. Govender et al (2008) suggested a strategy for the optimization of flocculation through the controlled expression of FLO1, FLO5, and FLO11 in S. cerevisiae [65].…”

Section: Flocculationmentioning

confidence: 99%

Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

et al. 2020

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The biorefinery concept, consisting in using renewable biomass with economical and energy goals, appeared in response to the ongoing exhaustion of fossil reserves. Bioethanol is the most prominent biofuel and has been considered one of the top chemicals to be obtained from biomass. Saccharomyces cerevisiae, the preferred microorganism for ethanol production, has been the target of extensive genetic modifications to improve the production of this alcohol from renewable biomasses. Additionally, S. cerevisiae strains from harsh industrial environments have been exploited due to their robust traits and improved fermentative capacity. Nevertheless, there is still not an optimized strain capable of turning second generation bioprocesses economically viable. Considering this, and aiming to facilitate and guide the future development of effective S. cerevisiae strains, this work reviews genetic engineering strategies envisioning improvements in 2 nd generation bioethanol production, with special focus in process-related traits, xylose consumption, and consolidated bioprocessing. Altogether, the genetic toolbox described proves S. cerevisiae to be a key microorganism for the establishment of a bioeconomy, not only for the production of lignocellulosic bioethanol, but also having potential as a cell factory platform for overall valorization of renewable biomasses.

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“…The FLO gene family can be divided into two groups. The first group-FLO1, FLO5, FLO9, and FLO10-share considerable sequence homology and are subtelomeric genes that code for proteins responsible for cell to cell adhesion and form aggregates of many cells known as flocs (Soares 2011;Di Gianvito et al 2017). These lectin-like proteins recognize and bind to α-mannan residues (receptors) of neighboring cells (Verstrepen et al 2003).…”

Section: Yeast Adhesionmentioning

confidence: 99%

New insights on yeast and filamentous fungus adhesion in a natural co-immobilization system: proposed advances and applications in wine industry

Ogawa

Bisson

García‐Martínez

et al. 2019

Appl Microbiol Biotechnol

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Fungi possess extraordinary strength in attachment to biotic and abiotic surfaces. This review focuses on adhesion mechanisms of yeast and filamentous fungi and the proposed combination of the adhesive forces of both organisms in an immobilization system called yeast biocapsules, whereby Saccharomyces cerevisiae cells are attached to the hyphae of Penicillium chrysogenum. The natural adherent properties of each organism, one multicellular and another unicellular, allow yeast to be fixated securely on the filamentous fungi and complete alcoholic fermentation. Following alcoholic fermentation, the hyphae become an inert support for yeast cells while maintaining shape and integrity. Biocapsules have been used successfully in both wine and bioethanol production. Investigation of the potential genes involved in fungal-yeast fusion suggests that natural hydrophobic interactions of both organisms play a major role. Analysis of the possible mechanisms involved in fungus and yeast adhesion, future perspectives on improving yeast immobilization, and proposed applications of the biocapsules are explored.

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FLO5 gene controls flocculation phenotype and adhesive properties in a Saccharomyces cerevisiae sparkling wine strain

Cited by 36 publications

References 64 publications

Multicellular Group Formation in <em>Saccharomyces cerevisiae</em>

Multicellular Group Formation in <em>Saccharomyces cerevisiae</em>

Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

New insights on yeast and filamentous fungus adhesion in a natural co-immobilization system: proposed advances and applications in wine industry

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