Flocculation and mechanism of self-flocculating lipid producer microalga Scenedesmus quadricauda for biomass harvesting

Aljuboori, Ahmad H. Rajab; Uemura, Yoshimitsu; Thành, Nguyễn Tiến

doi:10.1016/j.biombioe.2016.06.013

Cited by 38 publications

(21 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the 13 species identified in the present study, 5 have been studied in order to identify fatty acids for the production of biodiesel, so that in Table 5, it can be seen that the lipid content in the identified microalgae of hydrographic areas of the state of Chiapas can be from 2% to 90%, according to studies developed by Bogen et al (2013) , Rajab Aljuboori et al (2016) , Bonnefond et al (2016) and Sattar Memon et al (2016) , Chlorococcum and Monoraphidium being the most abundant in this study, with important contents of lipids reported (Table 5 ).…”

Section: Discussionmentioning

confidence: 82%

Evaluation of the population dynamics of microalgae isolated from the state of Chiapas, Mexico with respect to the nutritional quality of water

Roque¹,

Pérez-Luna²,

Acosta³

et al. 2018

BDJ

View full text Add to dashboard Cite

As Chiapas state, México, counts on an extensive hydrography with diverse nutrimental and climatic characteristics, it therefore allows isolating and identifying microalgae with bioenergetics potential.For this purpose, samples from 8 locations were collected, corresponding to 6 rivers, a wastewater and a springwater. The isolation of microalgae was developed for 4 weeks with 12:12 light/dark cycles. We demonstrated that the most efficient means for the isolation of microalgae of the hydrographic areas evaluated was the medium BG11 with 80.53% effectiveness. Of the microalgal consortium identified, 90% are composed of microalgae belonging to the class Chlorophycear. It was shown that another factor favouring the richness of morphotypes identified in the Santo Domingo River is associated with adequate concentrations of macroelements such as nitrates, nitrites, ammonium, phosphorus, sodium, potassium, magnesium and calcium at concentrations of 0.03 mg/l, 0.0006 mg/l, 0.08 mg/l, 0.03 mg/l, 62.93 mg/l, 5.46 mg/l, 34.52 mg/l and 48.78 mg/l respectively and microelements such as copper, zinc, iron, andmanganese at concentrations less than 0.2 mg/l in all microelements. The identified morphotypes, according to literature, have lipid contents ranging from 2 to 90%; this is of biotechnological importance for the production of biodiesel.

show abstract

Section: Discussionmentioning

confidence: 82%

Evaluation of the population dynamics of microalgae isolated from the state of Chiapas, Mexico with respect to the nutritional quality of water

Roque¹,

Pérez-Luna²,

Acosta³

et al. 2018

BDJ

View full text Add to dashboard Cite

show abstract

“…A limited number of studies on fungal bioflocculant have been reported (Table 3) [3,[59][60][61][62][63][64][65][66][67][68]. Among the isolated fungal strains, Aspergillus flavus was shown to produce a bioflocculant composed mainly of polysaccharide (69.7%) and protein (28.5%), with an excellent FA (<90%) without cation addition.…”

Section: Fungi and Microalgaementioning

confidence: 99%

“…However, another strain, Scenedesmus quadricauda, was shown to produce significant amounts of bioflocculant composed of sugar (56.7%) and protein (41%) [68]. The self-flocculation efficiency reached 96.8% at pH 7 with biomass concentration 0.21-39 g/L [67].…”

Section: Fungi and Microalgaementioning

confidence: 99%

Microbial Flocculants as an Alternative to Synthetic Polymers for Wastewater Treatment: A Review

2018

View full text Add to dashboard Cite

Microorganisms such as bacteria, fungi, and microalgae have been used to produce bioflocculants with various structures. These polymers are active substances that are biodegradable, environmentally harmless, and have flocculation characteristics. Most of the developed microbial bioflocculants displayed significant flocculating activity (FA > 70–90%) depending on the strain used and on the operating parameters. These biopolymers have been investigated and successfully used for wastewater depollution in the laboratory. In various cases, selected efficient microbial flocculants could reduce significantly suspended solids (SS), turbidity, chemical oxygen demand (COD), total nitrogen (Nt), dye, and heavy metals, with removal percentages exceeding 90% depending on the bioflocculating materials and on the wastewater characteristics. Moreover, bioflocculants showed acceptable results for sludge conditioning (accepted levels of dry solids, specific resistance to filtration, moisture, etc.) compared to chemicals. This paper explores various bioflocculants produced by numerous microbial strains. Their production procedures and flocculating performance will be included. Furthermore, their efficiency in the depollution of wastewater will be discussed.

show abstract

“…Despite the progress in using cell occulation of microalgae for biomass recovery, in-depth studies on mechanisms of microalgal cell occulation are still lacking. So far, microalgal cell wall polysaccharide and other extracellular polymeric substances (EPS) containing sugars and proteins in microalgae have been identi ed as occulating agents [10][11]13], but genes involved in the process remain unexplored. Identi cation of genes involved in microalgal cell self-occulation is of importance to develop robust strains with controlled occulation phenotype for microalgal biore nery, which can be enabled by the advancement of genetic engineering of microalgae [14].…”

Section: Introductionmentioning

confidence: 99%

Genome sequencing, assembly, and annotation of the self-flocculating microalga Scenedesmus obliquus AS-6-11

Chen

Mhuantong²,

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Scenedesmus obliquus belongs to green microalgae and is widely used in aquaculture as feed, which is also explored for lipid production and bioremediation. However, genomic studies of this microalga have been very limited. Cell self-flocculation of microalgal cells can be used as a simple and economic method for harvesting biomass, and it is of great importance to perform genome-scale studies for the self-flocculating S. obliquus strains to promote their biotechnological applications. Results We sequenced the genome of the self-flocculating microalga S. obliquus AS-6-11 employing the Pacific Biosciences sequencing platform, and used the MECAT software for de novo genome assembly. The estimated genome size of S. obliquus AS-6-11 is 172.3 Mbp with an N50 of 94,410 bp, and 31,964 protein-coding genes were identified. Gene Ontology (GO) and KEGG pathway analysis revealed 65 GO terms and 428 biosynthetic pathways. Comparing to the well-studied green microalgae Chlamydomonas reinhardtii, Chlorella variabilis, Volvox carteri and Micractinium conductrix, the genome of S. obliquus AS-6-11 encodes more unique proteins, and some of them might be responsible for the self-flocculating phenotype. These genes encode D-mannose binding lectin, glycosylphosphatidylinositol (GPI)-anchored cell wall proteins, and proteins with fasciclin domains that are commonly found in cell surface proteins, respectively. Four genes encoding both GPI-anchored cell wall proteins and fasciclin domain proteins are the most interesting targets for further studies. Conclusions To our best knowledge, this is the first report on the in-depth annotation of the S. obliquus genome, and the results will facilitate functional genomic studies of this important microalga. The comparative genomic analysis here also provides new insights into the evolution of green microalgae. Furthermore, identification of the potential genes encoding proteins for the self-flocculation of microalgal cells will benefit studies on the molecular mechanism underlying this phenotype for its better control and biotechnological applications as well.

show abstract

Flocculation and mechanism of self-flocculating lipid producer microalga Scenedesmus quadricauda for biomass harvesting

Cited by 38 publications

References 22 publications

Evaluation of the population dynamics of microalgae isolated from the state of Chiapas, Mexico with respect to the nutritional quality of water

Evaluation of the population dynamics of microalgae isolated from the state of Chiapas, Mexico with respect to the nutritional quality of water

Microbial Flocculants as an Alternative to Synthetic Polymers for Wastewater Treatment: A Review

Genome sequencing, assembly, and annotation of the self-flocculating microalga Scenedesmus obliquus AS-6-11

Contact Info

Product

Resources

About