Flocculation phenomenon of Candida albicans by lysozyme

Kamaya, T.

doi:10.1007/bf02129878

Cited by 6 publications

(6 citation statements)

References 17 publications

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“…In the presence of 1,000 µg/ml lysozyme, the turbidity of the washing media did not increase, which suggests low yeast cell loss by the biofilm. Previous studies have focused on the agglutination or flocculation power of lysozyme (at a cited concentration of 1 mg/ml) in non-ionic solutions upon air saprophytes (22), bacterial suspensions (38) or C. albicans yeasts (22). The presence of lysozyme in the in vivo acquired exogenous pellicle on teeth is well documented but no data concerning its effect on biofilm formation exist to date (39).…”

Section: Discussionmentioning

confidence: 99%

“…The three modes of action of lysozyme on C. albicans are its muramidase-like activity (19,20), its cationic nature capable of destabilizing the cell membrane (21), and its agglutination property (22). Although no peptidoglycan substrate for muramidase activity exists in C. albicans , lysozyme-induced wall-like material deposits between its chitin wall and its cell membrane have been observed by electron microscopy (19).…”

Section: Introductionmentioning

confidence: 99%

“…The in vitro effect of lysozyme on C. albicans biofilms remains controversial: certain studies demonstrated no beneficial effect of artificial saliva-containing lysozyme and other salivary proteins on yeast adhesion to acrylic resin (35), while others demonstrated a preventive role for lysozyme with respect to C. albicans biofilm formation on dentures (33). To the best of our knowledge, no studies have explored the link between C. albicans biofilm formation and lysozyme agglutination properties, previously described at 1 mg/ml concentration (22). The aim of the present in vitro study was to investigate whether lysozyme modulates C. albicans biofilm production when present in physiological concentrations, by triggering distinct mechanisms, namely inhibition and aggregation.…”

Section: Introductionmentioning

confidence: 99%

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Dose-dependent effect of lysozyme upon Candida albicans biofilm

Sebaa

Hizette

Boucherit-Otmani

et al. 2017

Molecular Medicine Reports

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The present study investigated the in vitro effect of lysozyme (0–1,000 µg/ml) on Candida albicans (C. albicans) biofilm development. Investigations were conducted on C. albicans ATCC 10231 and on 10 clinical isolates from dentures. Strains were cultured aerobically at 37°C in Sabouraud broth. Yeast growth was evaluated by turbidimetry. Biofilm biomass was quantified on a polystyrene support by crystal violet staining and on acrylic surfaces by counts of colony forming units. Lysozyme affected biofilm formation to a greater extent than it affected growth. For the ATCC 10231 reference strain, lysozyme acted as a biofilm promotor on polystyrene at the highest concentration tested (1,000 µg/ml, non-physiological). When the reference strain was investigated on acrylic resin support, lysozyme acted as a significant biofilm promotor on rough resin, but less on smooth resin. The attached biomass in the presence of physiological concentrations of lysozyme (10–30 µg/ml) was significantly decreased compared with the hypothetical value of 100% using a one-sample t-test, but a comparison between the different lysozyme conditions using analysis of variance and post hoc tests did not reveal significant differences. In 10 wild strains, different patterns of biofilm formation on polystyrene were observed in the presence of lysozyme. Some strains, characterized by large amounts of biofilm formation in the presence of 1,000 µg/ml lysozyme, were poor biofilm producers at low concentrations of lysozyme. In contrast, some strains that were poor biofilm producers with a high lysozyme concentration were more inhibited by low concentrations of lysozyme. The present study emphasizes the need to develop strategies for biofilm control based on in vitro experiments, and to implement these in clinical trials prior to approval of hygiene products enriched with exocrine proteins, such as lysozyme. Further studies will extend these investigations to other Candida species, and to fungi and bacteria present in oral biofilms.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dose-dependent effect of lysozyme upon Candida albicans biofilm

Sebaa

Hizette

Boucherit-Otmani

et al. 2017

Molecular Medicine Reports

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“…Several basic proteins including lysozyme have been shown to cause the release of ultraviolet-absorbing material from C. utilis with an accompanying decline in viability (26). Lysozyme binds to the hyphal growing tip of soil fungi (15) and leads to agglutination of living cells of some yeasts including C. albicans and C. stellatoidea (9). Whether the latter contribute to an antifungal activity of this enzyme is uncertain.…”

Section: Discussionmentioning

confidence: 99%

Effects of Lysozyme and Chitinase on the Spherules of Coccidioides immitis In Vitro

Collins

Pappagianis

1973

Infect Immun

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Spherules of Coccidioides immitis strain Silveira produced in vitro were treated with chitinase and lysozyme. The walls of merthiolate-killed mature endosporulating spherules were degraded by chitinase (500 ug/ml) and by lysozyme (100 and 500 Ag/ml). Thus, as was visible through the light microscope, the spherule wall was reduced in thickness from 1 to 2 ,m to less than 0.5 ,m. The degradation was evident also by release of N-acetylglucosamine, three times as much N-acetylglucosamine being released by chitinase in 12 h as was released by lysozyme in 3 days. However, the effect of lysozyme on living mature spherules was in marked contrast to the effect of chitinase in that treatment with lysozyme led to marked reduction in viability. Exposure to lysozyme (500 sg/ml) for 48 h permitted survival of only 0 to 0.2% of spherules. Thinning of the walls was observed only in the larger spherules (25-35 Mm) treated with lysozyme. By contrast, chitinase (500 ug/ml) led to complete dissolution of the walls of living mature spherules but the viability of the liberated endospores was unaffected during contact with chitinase for 48 h. Living non-endosporulating immature spherules and free endospores were also rendered nonviable by lysozyme but not by chitinase. [237][238][239][240][241][242] L. Ajello (ed.), Coccidioidomycosis. University of Arizona Press, Tucson. 26. Yphantis, D. A., J. L. Dainko, and F. Schlenk. 1967. Effect of some proteins on the yeast cell membrane. J. Bacteriol. 94:1509-1515. on July 15, 2020 by guest http://iai.asm.org/ Downloaded from

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“…Additionally, the use of the same enzyme without catalytic activity (after heat‐induced inactivation) also resulted in a similar effect, indicating that the mechanism was not enzymatic. The only report of lysozyme flocculating activity in the literature is by Kamaya et al on yeast ( Candida albicans ) and a more recent study explored the adsorption of lysozyme on silica nanoparticles and noted the aggregation of such particles by lysozyme . However, the flocculation activity of lysozyme on inorganic and other organic substrates has neither been further studied nor quantified.…”

Section: Introductionmentioning

confidence: 99%

Flocculation with Lysozyme: A Non‐Enzymatic Application

Bonilla

Allen

2016

Can J Chem Eng

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Bioflocculants have been previously recognized as an alternative to synthetic polymers due to their renewable sources and biodegradability. In this study, the flocculating activity of lysozyme on kaolin suspensions was investigated and compared to a known flocculant, a cationic polyacrylamide. Lysozyme was found to significantly aid in the flocculation of kaolin particles. The flocculating activity of lysozyme on kaolin was affected by the pH of the suspensions, while the addition of Mg2+ and Ca2+ ions did not have a significant effect. Potential industrial applications such as the flocculation of powdered activated carbon and algae suspensions were investigated and the addition of lysozyme resulted in significant flocculation. Furthermore, the flocculating properties of lysozyme are independent of its enzymatic activity. Our results indicate that lysozyme acts as a low molecular mass cationic polymer, where the main mechanism for flocculation on negatively‐charged particles is charge neutralization. Other cationic proteins could potentially be used as bioflocculants to enhance solid‐liquid separation.

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Flocculation phenomenon of Candida albicans by lysozyme

Cited by 6 publications

References 17 publications

Dose-dependent effect of lysozyme upon Candida albicans biofilm

Dose-dependent effect of lysozyme upon Candida albicans biofilm

Effects of Lysozyme and Chitinase on the Spherules of Coccidioides immitis In Vitro

Flocculation with Lysozyme: A Non‐Enzymatic Application

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