Floral authentication of Greek unifloral honeys based on the combination of phenolic compounds, physicochemical parameters and chemometrics

Karabagias, Ioannis K.; Vavoura, Maria V.; Nikolaou, Christos; Badeka, Anastasia V.; Kontakos, Stavros; Kontominas, Michael G.

doi:10.1016/j.foodres.2014.04.015

Cited by 80 publications

(59 citation statements)

References 39 publications

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“…Linear discriminant analysis (LDA) by the stepwise method was carried out to evaluate whether BLC samples from different regions could be mathematically distinguished on the basis of elements which had significant differences among the regions. The statistical significance of each discriminant function was evaluated on the basis of the Wilks' Lambda factor (Karabagias et al, 2014;Zhao et al, 2011). Predictive ability of LDA model was evaluated by leave-one-out cross-validation.…”

Section: Discussionmentioning

confidence: 99%

Determining the geographical origin of Chinese green tea by linear discriminant analysis of trace metals and rare earth elements: Taking Dongting Biluochun as an example

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Determining the geographical origin of Chinese green tea by linear discriminant analysis of trace metals and rare earth elements: Taking Dongting Biluochun as an example

et al. 2016

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“…The analytical procedures used to determine polyphenols in honey involve their extraction from the honey matrix, and their chromatographic separation followed by quantification . Numerous studies of honey phenolic acids and flavonoids have been focused on the extraction of the phenolic compounds from honey using the Amberlite XAD‐2 resin, solid‐phase extraction (SPE) procedures with commercial cartridges (Bond Elut octadecyl C18, Sep‐Pak RP C18, Oasis HLB and Strata‐X) or liquid–liquid extraction methods prior to their identification and quantification. Liquid chromatography (LC) is considered to be the most useful separation technique for the analysis of polyphenols in honey, including high‐performance liquid chromatography diode array detector (HPLC‐DAD) and HPLC‐mass spectrometry (MS) for quantitative measurements …”

Section: Introductionmentioning

confidence: 99%

Phenolic compounds profile and biochemical properties of honeys in relationship to the honey floral sources

Ciucure

Geană

2019

Phytochemical Analysis

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Introduction Honey has been considered to have therapeutic properties since ancient times and among the factors responsible for such activity are phenolic compounds including phenolic acids and flavonoids from different natural sources. Objective This study investigated the phenolic compounds profile and bioactive properties of different honey types from Romanian flora in order to develop reliable tools for honey floral origin, thus contributing to the honey traceability in the European Union context. Material and methods Thirty‐three honey samples were examined, including unifloral (acacia and rape), polyfloral, honeydew honeys and mixture honeys. Phenolic acids and flavonoids were isolated from the water soluble honey matrix using a solid‐phase extraction (SPE) method and analysed by ultra‐high‐performance liquid chromatography diode array detector electrospray ionisation mass spectrometry (UHPLC‐DAD‐ESI/MS). Honey bioactive properties were measured in honey dissolved in 80% ethanol using UV‐visible spectrophotometric methods. Multivariate statistical tools (principal component analysis and hierarchical clustering analysis) were used for honey classification. Results The results of this study confirm that honey samples had similar, but quantitatively different, phenolic acids and flavonoids profiles and bioactive properties, related with honey floral source. Coloured honeys, such as honeydew honey, show high phenolic composition and bioactive properties and implicitly a high therapeutic potential compared with the other floral honeys. Conclusion Distinctive clusters obtained by principal component analysis enabled us to consider that honeydew and polyfloral honeys could be distinguished from acacia and rape honey with the analytical methods developed. Based on this study, the methods might be promising tools for honey traceability, which needs to be explored on a larger set of samples with different regional floral origins in future studies.

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“…in several fir FHH from Slovenia by LC‐DAD/ESI‐MS. Various authors have determined physical‐chemical properties such as color, pH, conductivity of the samples from Slovenia and Greece . However, the data available to date are inconsistent and show relevant differences between phytochemical profiles and physical‐chemical properties reported by different authors, especially regarding FHH of different geographical origin.…”

Section: Introductionmentioning

confidence: 99%

Screening of Polish Fir Honeydew Honey Using GC/MS, HPLC‐DAD, and Physical‐Chemical Parameters: Benzene Derivatives and Terpenes as Chemical Markers

Kuś

Jerković

Marijanović

et al. 2017

Chemistry & Biodiversity

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GC/MS of headspace solid phase micro extraction (HS-SPME) and solvent extractives along with targeted HPLC-DAD of Polish fir (Abies alba Mill.) honeydew honey (FHH), were used to determine the chemical profiles and potential markers of botanical origin. Additionally, typical physical-chemical parameters were also assigned. The values determined for FHH were: conductivity (1.2 mS/cm), water content (16.7 g/100 g), pH (4.5), and CIE chromaticity coordinates (L* = 48.4, a* = 20.6, b* = 69.7, C* = 72.9, and h° = 73.5). FHH contained moderate-high total phenolic content (533.2 mg GAE/kg) and antioxidant activity (1.1 mmol TEAC/kg) and (3.2 mmol Fe /kg) in DPPH and FRAP assays. The chemical profiles were dominated by source plant-originated benzene derivatives: 3,4-dihydroxybenzoic acid (up to 8.7 mg/kg, HPLC/honey solution), methyl syringate (up to 14.5%, GC/solvent extracts) or benzaldehyde (up to 43.7%, GC/headspace). Other markers were terpenes including norisoprenoid (4-hydroxy-3,5,6-trimethyl-4-(3-oxobut-1-enyl)cyclohex-2-en-1-one, up to 20.3%, GC/solvent extracts) and monoterpenes, mainly linalool derivatives (up to 49%, GC/headspace) as well as borneol (up to 5.9%, GC/headspace). The application of various techniques allowed comprehensive characterisation of FHH. 4-Hydroxy-3,5,6-trimethyl-4-(3-oxobut-1-enyl)cyclohex-2-en-1-one, coniferyl alcohol, borneol, and benzaldehyde were first time proposed for FHH screening. Protocatechuic acid may be a potential marker of FFH regardless of the geographical origin.

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Floral authentication of Greek unifloral honeys based on the combination of phenolic compounds, physicochemical parameters and chemometrics

Cited by 80 publications

References 39 publications

Determining the geographical origin of Chinese green tea by linear discriminant analysis of trace metals and rare earth elements: Taking Dongting Biluochun as an example

Determining the geographical origin of Chinese green tea by linear discriminant analysis of trace metals and rare earth elements: Taking Dongting Biluochun as an example

Phenolic compounds profile and biochemical properties of honeys in relationship to the honey floral sources

Screening of Polish Fir Honeydew Honey Using GC/MS, HPLC‐DAD, and Physical‐Chemical Parameters: Benzene Derivatives and Terpenes as Chemical Markers

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