Floral biology and pollen viability of parental lines of onion hybrids

Ali, Muhammad; Dowker, B. D.; Currah, L.; Mumford, Pauline M.

doi:10.1111/j.1744-7348.1984.tb05599.x

Cited by 9 publications

(7 citation statements)

References 8 publications

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“…The correlation between the percentage of stainable pollen and germination was significant (P < 0.001), although it was only 0.45. Other researchers have reported low correlations between pollen germination and stainability using acetocarmine, cotton blue, or fluorescein diacetate (Ali et al, 1984;Gates, 1976a, 1976b). Overall, these previous studies reported pollen germination rates of 50% or less, and it is not clear if the low germination was due to the medium type, amounts of sucrose and/or microelements, or other factors (Gomes et al, 2000;Ockendon and Gates, 1976a).…”

Section: Resultsmentioning

confidence: 96%

Variable Penetrance among Different Sources of the Male Fertility Restoration Allele of Onion

HsiangI¹,

Havey²

2020

horts

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Hybrid onion (Allium cepa) seed is produced using cytoplasmic male sterility (CMS). For the most commonly used source of onion CMS, male fertile plants possess male sterile (S) cytoplasm and dominant allele(s) at one nuclear male fertility locus (Ms). Because male fertility restoration is not necessary for bulb production, it is desirable to purge dominant alleles at Ms from populations and breeding lines to facilitate the development of male sterile inbreds for hybrid production. In this research, we used molecular markers to establish the cytoplasms and genotypes at Ms in progenies from testcrosses of male sterile lines with plants from three populations [B2354, Ailsa Craig (AC), and Sapporo-Ki (Ski)] possessing the dominant Ms allele. We scored male fertility of testcross progenies by visual examination of flowers and acetocarmine staining of pollen. Different sources of the dominant Ms allele showed significantly different amounts of male fertility restoration and proportions of stainable pollen, complicating visual selection against the dominant Ms allele. For AC and Ski, molecular markers correctly predicted male sterility vs. male fertility of progenies in the greenhouse and field. However, for B2354, male fertility restoration was less clear and especially difficult to score under field conditions, consistent with reduced penetrance of male fertility restoration for this source of the dominant Ms allele. These results will be of interest to onion breeders selecting S-cytoplasmic male sterile lines for hybrid onion development.

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Section: Resultsmentioning

confidence: 96%

Variable Penetrance among Different Sources of the Male Fertility Restoration Allele of Onion

HsiangI¹,

Havey²

2020

horts

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“…A média de flores por umbela registrada na literatura é variável, a exemplo de 612,2 para Crioula em Santa Catarina (Müller & Casali 1982) e de outras cultivares híbridas que apresentaram de 213 a 485 flores por umbela (Currah 1981, Ali et al 1984e Lorenzon & Almeida 1993.…”

Section: Resultsunclassified

“…De acordo com outros autores, a duração do episódio de floração em uma umbela pode variar de 16 a 35 dias (Currah & Ockendon 1978, Ali et al 1984, Acosta et al 1993.…”

Section: Resultsunclassified

“…Lorenzon & Almeida (1997) e Ockendon & Gates (1976), testando a viabilidade do pólen de cebola com corantes, registraram elevados índices de grãos com conteúdo citoplasmático, entre 84% e 99%. Em contrapartida, percentuais de viabilidade de pólen de cebola foram inferiores quando testados com fluoresceína, que destaca a integridade do plasmalema (Ockendon & Gates 1976, Ali et al 1984. Segundo Ockendon & Gates (1976), este método possibilita uma melhor indicação de viabilidade do pólen do que a simples presença de citoplasma.…”

Section: Resultsunclassified

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Progressão da floração e antese de Allium cepa L. (Alliaceae) em Candiota, RS, Brasil

Witter¹,

Wittmann²,

Blochtein³

2005

Rev. bras. Bot.

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-(Progression of flowering and anthesis of Allium cepa L. (Alliaceae) in Candiota, RS, Brasil). The necessity for pollination by insects depends on the morphology of the flower, the degree of self-fertility and the arrangement of the flowers. We studied the progression of the flowering of Allium cepa L., by measuring the frequency of visits by Apis mellifera L., the anthesis phase and pollen viability. The flowering period in the cultivar Crioula (56 days) was longer than in Bola Precoce (50 days). The duration of the peak of flowering was similar in both cultivars, 15 and 17 days respectively. The correlation between the frequency of visits by A. mellifera and the number of umbels with open flowers was high in Crioula and average in Bola Precoce. The anthesis occured at 7 and 8 days respectively in the two cultivars. Contrary to other observations, we have verified incomplete protandry in both cultivars. Stigmas were receptive when the styles had reached a length of 4-5.5 mm. The average percentage of pollen viability was 90.46 (Crioula) and 80.25 (Bola Precoce).

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“…and is greatly affected by environment, age and the position within the plant [25]. In KP onion, floral differentiation and organogenesis occur simultaneously with both scape elongation and vegetative growth and development as reported in other varieties of onion [7].…”

Section: Resultsmentioning

confidence: 99%

Florogenesis and Female Gametophyte Development in <i>Allium cepa</i> L. cv. Krishnapuram

Lakshmi¹,

Raja²,

Naik³

et al. 2017

AJPS

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Florogenesis is one of the most complicated and interesting processes in the nature. This process involves developmental, physiological and molecular events leading to transformation from vegetative to reproductive phase for optimal seed production and the continuation of species. The basic knowledge about flowering processes, male and female sexual systems support basic and applied research and breeding programs. Most of the onion varieties from India are short day varieties, more diverse than other exotic germplasm and useful as a source of new alleles for supporting breeding programs. The present investigation was focused to study for the first time florogenesis process by scanning electron study for the first time florogenesis process by scanning electron microscopy (SEM) and development of female gametophytes by light microscopy in order to acquire basic knowledge useful for optimizing in vitro process to produce gynogenic haploid to support and speed breeding program in short-day onion Allium cepa L. cv. Krishnapuram (KP) or Bangalore Rose.This study revealed that shoot primordium differentiated into inflorescence meristem in the month of December, while seeds were planted in the field in the September. The individual florets are preceded by a variying number of floral initials. The female gametophyte developed from chalazal side megaspore. The embryo sac development is a bisporic Allium type showing shortlived antipodals. The histological study suggests that the use of big or preanthesis flower buds with embryo sac for production of gynogenic haploids to support breeding program in onion cv. Krishnapuram (KP). However further studies are needed for confirmation of this observation.

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Floral biology and pollen viability of parental lines of onion hybrids

Cited by 9 publications

References 8 publications

Variable Penetrance among Different Sources of the Male Fertility Restoration Allele of Onion

Variable Penetrance among Different Sources of the Male Fertility Restoration Allele of Onion

Progressão da floração e antese de Allium cepa L. (Alliaceae) em Candiota, RS, Brasil

Florogenesis and Female Gametophyte Development in <i>Allium cepa</i> L. cv. Krishnapuram

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Floral biology and pollen viability of parental lines of onion hybrids

Cited by 9 publications

References 8 publications

Variable Penetrance among Different Sources of the Male Fertility Restoration Allele of Onion

Variable Penetrance among Different Sources of the Male Fertility Restoration Allele of Onion

Progressão da floração e antese de Allium cepa L. (Alliaceae) em Candiota, RS, Brasil

Florogenesis and Female Gametophyte Development in &lt;i&gt;Allium cepa&lt;/i&gt; L. cv. Krishnapuram

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Florogenesis and Female Gametophyte Development in <i>Allium cepa</i> L. cv. Krishnapuram