Floral development in Hura crepitans (Euphorbiaceae): a bat-pollinated species with multicarpellate gynoecium

Daphnanes diterpenes from the latex of Hura crepitans L. and their PKCζ-dependent anti-proliferative activity on colorectal cancer cells

“…Hura crepitans L. is a tree of the Euphorbiaceae, a large family with more than 3000 genus and 8000 species [1][2][3][4] . This thorn-covered tree is widespread in South America, Africa and Asia and produces an irritating milky latex containing numerous secondary metabolites, notably diterpenes [5][6][7][8] .…”

“…In this paper, we describe the isolation and structural elucidation of five new analogs of huratoxin (6'R-hydroxy-huratoxin (1), 4'S,5'S-dihydroxy-huratoxin (2), 4'(Z)-6'-oxohuratoxin (3), 4'S,5'S-epoxy-huratoxin (4), and huratoxigenin-20-(11'-methyloctadec-12'enoate) (5)) along with two known daphane-type diterpenes (huratoxin (6) and 6'-oxohuratoxin (7)) from the latex of H. crepitans (Fig. 1).…”

“…Figure 15: Cytostatic activity of huratoxin and 4',5'-epoxy-huratoxin on 3D cultures of human primary colorectal cells.Colorectal epithelial cells were isolated from control(1,2, 3) and CRC patients(4,5) and cultured as organoids ex vivo. After 48 h of organoid formation from individual cells, incubation with huratoxin, epoxy-huratoxin, 5FdU or DMSO (Control) for 48 h was performed.…”