Floral-specific polypeptides of the Japanese morning glory

Abstract

Polypeptides from stems, leaves, sepals, corollas, stamens and pistils of the Japanese morning glory (Ipomoea nil Roth (Pharbitis nil Chois.)) were separated by one- and two-dimensional gel electrophoresis and visualized by silver staining. The majority of polypeptides were expressed in two or more organs, while those specific to only one organ were comparatively rate. Among the polypeptides of the former class were two which appeared to be floral-specific. A 46-kDa (kilodalton) polypeptide was expressed in corollas, stamens and pistils, whereas a 32-kDa polypeptide was observed only in extracts prepared from reproductive organs. Polypeptide spots from the various organs were compared with those from leaves, and it was found that sepals and stems shared 40-50% of their polypeptides with leaves, whereas corollas, stamens and pistils shared 20% or less. The latter organs shared 120 polypeptides or roughly 15% of those identified in the floral extracts. Floralorgan-specific polypeptides comprised nearly 10% of the total floral polypeptides identified.

Polypeptide profiles from cotyledons of developing and photoperiodically induced seedlings of the Japanese morning glory (Pharbitis [ipomoea] nil)

“…In preparation for extraction, they were transferred from -80~ to liquid nitrogen, quickly weighed to minimize thawing, and immediately returned to liquid nitrogen. Sodium dodecyl sulfate-soluble protein was isolated, quantified, and stored as previously described (Bassett et al 1988), except that 40 p.g/ml bestatin and 0.7 i~g/ml pepstatin were included in the extraction buffer of samples from one of the experiments (the one whose results are reported here) to analyze age-related changes during growth under noninductive conditions.…”

“…Standard equilibrium two-dimensional gel electrophoresis (2-D PAGE) was performed as described (Bassett et al 1988). Isoelectric focusing (IEF) marker proteins (BioRad) were included in one gel of each batch of IEF gels, and the pH gradient was confirmed in each run by slicing and eluting a control gel.…”

“…Chois Roth]) were obtained from the Marutane Seed Co., Kyoto, Japan and were selected for uniform size. They were imbibed and germinated in the dark for 1 day as previously described (Bassett et al 1988), except that coarse sand was included in the water wash to improve scarification. At t day postimbibition (DPI), well-germinated seeds, with radicles protruding 2-5 ram, were planted in Fafard No.…”

Plant and organ development

“…In a comparison of the protein composition of the floral organs of several solanaceous species, one-dimensional SDS-PAGE was inadequate to show clear and consistent differences between organ types, and no organ-specific polypeptides were detected [10]. 55 In a more detailed analysis of the protein composition of the floral organs of Pharbitis with twodimensional PAGE, the number of spots varied from 209 for the corolla extracts to 479 for the pistil [4]. Of about 800 identifiable spots, only about 4% or about 30 in each floral organ (corolla, stamen, pistil) were unique to each organ (Fig.…”

Immunological Characterization of a Tapetal Protein in Developing Anthers of Lilium longiflorum

“…Support for this opinion comes from recent studies on anther-specific genes (11,18,20,23) of mRNAs in lily, tobacco, and rice pollen (25,30). There is also a similar literature on organ-and tissue-specific proteins in Japanese morning glory (2,22), Tulipa (1,3), Gladiolus pollen and stigma (7), Lycopersicon flowers (28), Petunia anthers (24), carrot stamen (17), maize and tobacco pollen (8,35), and, most recently, of developing lily (Lilium longiflorum) anthers (33).…”