Floridean starch and carrageenan contents as responses of the red alga Solieria chordalis to culture conditions

FOURNET, ISABELLE; ZINOUN, MUSTAPHA; DESLANDES, ERIC; Diouris, M.; FLOC'H, JEAN YVES

doi:10.1017/s096702629900205x

Cited by 4 publications

(5 citation statements)

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“…Thus, the photosynthetic carbon was not oriented towards the synthesis of low-molecular-weight compounds but certainly aimed towards storage product like floridean starch or carrageenan. This hypothesis is supported by the study of Fournet et al (1999) which showed that S. chordalis synthesized floridean starch under the same conditions of irradiance when the alga was post-conditioned one week in the dark. After this first 24 h, the C/N ratio increased strongly to reach 1.93 (after 2.5 days) resulting in the orientation of photosynthetic carbon towards a carbonated compound, the floridoside for which the level was multiplied by 53 %.…”

Section: Effects Of Changing Culture Conditionsmentioning

confidence: 71%

The use of HRMAS NMR spectroscopy to study the in vivo intra–cellular carbon/nitrogen ratio of Solieria chordalis (Rhodophyta)

et al. 2007

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The red alga Solieria chordalis (J. Agardh) C. Agardh (Rhodophyta) was used as a model to investigate the effects of changes in seawater salinity on the carbon/ nitrogen ratio. Carbohydrates and nitrogenous metabolites are major components of this alga and their metabolisms are intimately linked. Previous publications have provided a solid database for these two primary metabolic pathways from experiments and observations in situ. Storage products (e.g. floridean starch), cell wall polysaccharides (carrageenan) and low molecular weight carbohydrates such as floridoside and digeneaside are major compounds constituting the pool of available carbon. Compounds such as amino acids and peptides, constitute the pool of nitrogen. This study focuses on the intracellular C/N ratio inside the pool of low molecular weight compounds. This C/N ratio can be defined as the balance between carbohydrates and amino acids. High-resolution magic angle spinning nuclear magnetic resonance spectroscopy (HRMAS NMR) provides a powerful approach for in vivo analysis of the pool of intracellular organic compounds. These in vivo results were complimented with quantitative data obtained from high performance liquid chromatography (HPLC). In vivo and in vitro experimental analyses provided a intracellular molecular balance and defined the C/N ratio. In order to study the effect of salt stress on the carbon/nitrogen ratio, S. chordalis was cultured under controlled conditions. Effects of hyposalinity and hypersalinity stresses (low 22 ‰ and high 50 ‰ salinity) were tested. Both HPLC and NMR data, obtained on stressed and unstressed algae, generated insights into variations of carbonated and nitrogenous metabolites, involving changes of the C/N ratios, and demonstrated the adaptive responses of the seaweed.

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Section: Effects Of Changing Culture Conditionsmentioning

confidence: 71%

The use of HRMAS NMR spectroscopy to study the in vivo intra–cellular carbon/nitrogen ratio of Solieria chordalis (Rhodophyta)

et al. 2007

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“…Fig. 4a groups [29,30]. These groups appear in algal Gelidium associated with sulphated galactans of agar.…”

Section: Ftir Characterizationmentioning

confidence: 95%

Copper removal by algal biomass: Biosorbents characterization and equilibrium modelling

Vilar

Botelho

Pinheiro

et al. 2009

Journal of Hazardous Materials

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“…The fragments were mixed together before being distributed to the culture flasks in order to obtain results representative of the population. We used the conditions that we had previously found as optimal for floridean starch synthesis or degradation (Fournet et al 1999). The algal fragments were placed in filtered seawater at 12°C and submitted to a 12:12 LD cycle for 20 days with an irradiance of 75 µmol photons m -2 s -1 .…”

Section: Culture Conditionsmentioning

confidence: 99%

“…Starch concentration was determined from the difference between the total glucose after amyloglucosidase hydrolysis and the free glucose in the lyophilized extract before amyloglucosidase hydrolysis. The procedure used was derived from Bergmeyer and Bernt by Fournet et al (1999).…”

Section: Floridean Starchmentioning

confidence: 99%

“…photon flux density and nutrient supply) on the NDPsugar pool size and on NDP-sugar pyrophosphorylases and UDP-glucose-4-epimerase activities. A previous study carried out in our laboratory had, indeed, shown that changes in light and nutriments result in either floridean starch biosynthesis or degradation (Fournet et al 1999). In addition, a high performance liquid chromatography (HPLC) method was developed to measure ADP and UDP-glucose pyrophosphorylase activities.…”

Section: Introductionmentioning

confidence: 99%

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NDP‐sugars, floridoside and floridean starch levels in relation to activities of UDP‐glucose pyrophosphorylase and UDP‐glucose‐4‐epimerase in Solieria chorda l is (Rhodophyceae) under experimental conditions

Goulard

Corre

Diouris

et al. 2001

Phycological Research

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SUMMARY Under limited nutrient availability (i.e. unenriched sea‐water) and under 75 mol photons m–2 s–1 irradiance 12:12 LD, thalli of Solieria chordalis J. Agardh accumulated floridean starch and floridoside. When they were transferred into nutrient‐enriched seawater (150 umol L−1 NO31‐ and 7 umol L−1 P043i at 35 umol photons m−2 s−1 in irradiance 12:12 LD, starch and floridoside levels decreased. The main nucleotide diphosphate (NDP) sugars (i.e. UDP‐glucose, UDP‐galactose and ADP‐glucose) and the activities of UDP‐glucose pyrophosphorylase [Enzyme Code (EC) 2.7.7.9] and UDP‐glucose‐4‐epimerase (EC 5.1.3.2) were measured under these controlled culture conditions. Both UDP‐glucose and UDP‐galactose in the thal l i increased under conditions known to favor the accumulation of floridean starch and floridoside, whereas they decreased under conditions leading to floridean starch and floridoside breakdown. On the other hand, ADP‐glucose level only varied slightly. Although UDP‐glucose pyrophosphorylase activity rose under conditions of floridean starch synthesis, little variation was observed in UDP‐glucose‐4‐epimerase activity. These results suggest a possible enzymatic regulation of the NDP‐sugar and carbohydrate pool in which UDP‐glucose pyrophosphorylase would play a major role.

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Floridean starch and carrageenan contents as responses of the red alga Solieria chordalis to culture conditions

Cited by 4 publications

References 0 publications

The use of HRMAS NMR spectroscopy to study the in vivo intra–cellular carbon/nitrogen ratio of Solieria chordalis (Rhodophyta)

The use of HRMAS NMR spectroscopy to study the in vivo intra–cellular carbon/nitrogen ratio of Solieria chordalis (Rhodophyta)

Copper removal by algal biomass: Biosorbents characterization and equilibrium modelling

NDP‐sugars, floridoside and floridean starch levels in relation to activities of UDP‐glucose pyrophosphorylase and UDP‐glucose‐4‐epimerase in Solieria chorda l is (Rhodophyceae) under experimental conditions

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