An experimental investigation of the foam separation of E . coli from distilled water suspension using a cationic surface-active agent, ethylhexadecyldimethylammonium bromide (EHDA-Br) is presented. Results are evaluated in terms of total cell count, using a membrane filtrat,ion t,echnique. Cell concentrations iri the initial suspensions are varied from 5.0 X lo5 to 1 .0 x 108 cells/ml. Surfactant concentrations in the initial cell suspensions are varied from 0.015 t,o 0.040 mg./ml., and foaming times are varied from 2 to 20 min. The residual quantity of cells decreases exponentially with foaming t,ime to about 0.027c of the initial quantit'y after 20 min. The cell enrichment rat,io, varying from 10 t,o 1,000,000, is an inverse power function of the initial stirfactant concentration and an exponential function of foaming time. Foaminess decreases with increasing initial cell concentrations, and for an initial surfactant concentration of 0.030 mg./ml., ()he residual cell concentration is a 1inea.r function of the initial cell concentration.