Flow analysis with chemiluminescence detection: Recent advances and applications

Timofeeva, Irina; Vakh, Christina; Bulatov, Andrey; Worsfold, Paul J.

doi:10.1016/j.talanta.2017.11.007

Cited by 60 publications

(27 citation statements)

References 254 publications

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“…This peculiar "light system" finds its widespread use in detection assays, especially in the biological samples analysis. Specific chemiluminescent compounds are used as a probe, as they emit light once they meet the analytes [17][18][19][20][21][22][23][24][25][26] . Nonetheless, in literature there can be found some studies in which CL is used as an excitation source in macro environments.…”

Section: Integrating a Light Source Inside A Lab-on-a-chip (Loc) Platmentioning

confidence: 99%

Battery-free fully integrated microfluidic light source for portable lab-on-a-chip applications

Storti

Bonfadini

Criante

2020

Sci Rep

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Integrating a light source inside a Lab-on-a-Chip (LOC) platform has always been as challenging as much as an appealing task. Besides the manufacturing issues, one of the most limiting aspects is due to the need for an energy source to feed the light emission. A solution independent of external energy sources can be given by Chemiluminescence (CL): a well-known chemical phenomenon in which light emission is achieved because of a chemical reaction. Here we present the fabrication and the characterization of a chemiluminescent light source, fully integrated on a microfluidic platform by means of the direct writing technique known as Femtosecond Laser Micromachining. The key advantage is the possibility to insert within LOC devices light sources with complete placement freedom in 3D, wide flexibility of the emitting source geometry and no external feeding energy. The characterization is carried out by investigating the effect of confining a chemiluminescent rubrene-based reaction in small volumes and the inject pressures impact on the emission spectra. Moreover, exploiting microfluidics principles, it’s possible to move from the typical flash-type CL emission to a prolonged one (several hours). This allows to disengage bulky, external light sources, adding an extra step on the road to real device portability.

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Section: Integrating a Light Source Inside A Lab-on-a-chip (Loc) Platmentioning

confidence: 99%

Battery-free fully integrated microfluidic light source for portable lab-on-a-chip applications

Storti

Bonfadini

Criante

2020

Sci Rep

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“…Flow injection chemiluminescence immunoassay (FI‐CLIA) is a chemical analysis technique combined with flow injection chemiluminescence and immunoassay, which overcomes the limitations, such as low sensitivity and poor specificity, of traditional analysis methods 20, 21 . This method is widely used in biological diagnosis, 22, 23 food monitoring, 24, 25 drug analysis, 26, 27 and in other fields 28 .…”

Section: Introductionmentioning

confidence: 99%

A simple and sensitive flow injection chemiluminescence immunoassay for chloramphenicol based on gold nanoparticle‐loaded enzyme

et al. 2020

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A simple and ultrasensitive flow injection chemiluminescence competitive immunoassay based on gold nanoparticle‐loaded enzyme for the detection of chloramphenicol (CAP) residues in shrimp and honey has been developed. Due to their good biocompatibility and large specific surface area, carboxylic resin beads can be used as solid phase carriers to immobilize more coating antigens (Ag). In addition, gold nanoparticles could provide an effective matrix for loading more CAP antibody and horseradish peroxidase, which would effectively catalyze the system of luminol–p‐iodophenol (PIP)–H2O2. A competitive immunoassay strategy was used for detection of CAP, in which CAP in the sample would compete with the coating Ag for the limited antibodies, leading to a chemiluminescence (CL) signal decrease with increase in CAP concentration. A wide linear range 0.001–10 ng ml−1 (R2 = 0.9961) was obtained under optimized conditions, and the detection limit (3σ) was calculated to be 0.33 pg ml−1. This method was also been successfully applied to determine CAP in shrimp and honey samples. The immunosensor proposed in this study not only has the advantages of high sensitivity, wider linear range, and satisfactory stability, but also expands the application of flow injection CL immunoassay in antibiotic detection.

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“…Chemiluminescence (CL) is widely applied chemically driven electromagnetic radiation for real-life applications, from clinical monitoring, food and pharmaceutical safety control, and up to trace levels of environmental pollutants analysis, utilizing its high sensitivity toward the assessment of the limiting target reactant involved in the luminol reaction [1,2,3,4,5,6,7,8,9,10,11]. The low emitted radiation within complex samples such as blood, milk, urine, or biological tissues, can be efficiently augmented by the addition of a catalyst, e.g., metal or semiconductor nanoparticles, catalytic ions, enzymes, into the luminol CL system.…”

Section: Introductionmentioning

confidence: 99%

Gold Nanoparticle Size-Dependent Enhanced Chemiluminescence for Ultra-Sensitive Haptoglobin Biomarker Detection

Nirala

Shtenberg

2019

Biomolecules

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Bovine mastitis (BM) is a frequent disease in the dairy industry that causes staggering economical losses due to decreased milk production and increased health care costs. Traditionally, BM detection depends on the efficacy and reliability of analytical techniques that measure somatic cell counts (SCC), detect pathogens, and reveal inflammatory status. Herein, we demonstrate the detection of bovine haptoglobin, a well-documented acute phase protein for evaluating BM clinical status, by utilizing hemoglobin-binding capacity within luminol chemiluminescence (CL) system. The resulting haptoglobin–hemoglobin complex reduces the CL signal proportionally to inherent haptoglobin concentrations. Different sizes of cross-linked gold nanoparticles (GNPs) were examined for enhanced CL (eCL) signal amplification, presenting over 30-fold emitted radiation enhancement for optimized size within real milk samples with respect to nanoparticle-free assay. The eCL values were proportionally related to nanoparticle size and content, influenced by SCC and pathogen type (e.g., Escherichia coli and coagulase-negative staphylococci). The optimized bioassay showed a broad linear response (1 pg mL−1–10 µg mL−1) and minute detection limit of 0.19 pg mL−1, while presenting quantitative performance in agreement with commercial ELISA kit. Finally, the resulting optimized eCL concept offers an efficient label-free detection of haptoglobin biomarker, offering means to diagnose the severity of the associated diseases.

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Flow analysis with chemiluminescence detection: Recent advances and applications

Cited by 60 publications

References 254 publications

Battery-free fully integrated microfluidic light source for portable lab-on-a-chip applications

Battery-free fully integrated microfluidic light source for portable lab-on-a-chip applications

A simple and sensitive flow injection chemiluminescence immunoassay for chloramphenicol based on gold nanoparticle‐loaded enzyme

Gold Nanoparticle Size-Dependent Enhanced Chemiluminescence for Ultra-Sensitive Haptoglobin Biomarker Detection

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