Flow characterization and patch clamp dose responses using jet microfluidics in a tubeless microfluidic device

Resto, Pedro J.; Bhat, Abhishek; Stava, Eric; Lor, Chong; Merriam, Elliot; Díaz-Rivera, Rubén E.; Pearce, Robert A.; Blick, Robert H.; Williams, J.

doi:10.1016/j.jneumeth.2017.08.025

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…The above outlined methods for creating neuronal circuits in vitro are now increasingly being integrated with MEAs for electrophysiological interrogation, combining the advantageous spatial cell patterning capabilities and precise control of chemical cues provided by microfluidics with electrical stimulation and recording of neuronal networks. Throughout the years, several methodologies have been developed, from manual whole cell patch clamping in standard two‐chamber microfluidic devices (Jokinen et al., 2013), to tubeless devices based on capillary forces for drug delivery (Resto et al., 2017), to microfluidic planar patch clamp systems (Xu et al., 2014) with automated drug delivery (Yuan et al., 2016). However, it is the integration of microfluidics with MEA substrates, possibly due to the planarity of both technologies, that has produced higher‐throughput and innovative computational solutions.…”

Section: Microfluidic Tools To Study Neuronal Functionmentioning

confidence: 99%

Advances in microfluidic in vitro systems for neurological disease modeling

Holloway

Willaime‐Morawek

Siow

et al. 2021

J of Neuroscience Research

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Section: Microfluidic Tools To Study Neuronal Functionmentioning

confidence: 99%

Advances in microfluidic in vitro systems for neurological disease modeling

Holloway

Willaime‐Morawek

Siow

et al. 2021

J of Neuroscience Research

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“…Based on recent progress in micro-electronic andfluidic device technology [19][20][21], in conjunction with RF-circuits [22][23][24][25] dielectric spectroscopy now holds huge potential as a noninvasive and label free characterization method for single cells [26,27]. An advanced approach based on tubular CPWs (T-CPWs), was demonstrated by Bausch et al Here, the tubular CPW was employed for T cell counting, enabling enhanced detection sensitivity in the radio-frequency range at 177 MHz [28].…”

Section: Introductionmentioning

confidence: 99%

Label-free single-cell counting and characterization in the GHz-range

et al. 2022

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We demonstrate operation of a micropore based flow cytometer in the radio-frequency range. Apart from simply counting micron sized particles, such as cells, with close to nano-second resolution this counter offers the additional benefit of delivering insight into the intracellular environment. Such non-invasive screening of the cell’s interior based on analysing amplitude and phase of the signal is helpful in characterizing the biological activity of cells. In detail we are using heterodyne mixing to demodulate the temporal impedance changes, which are induced by cells translocating through a micropore embedded in a radio-frequency circuit. This allows us to measure every amplitude and phase modulation induced by a translocation event. Herein, we compare the Jurkat cells (human T lymphocytes) recordings with a control group of polystyrene beads. As the cells are measured on a single cell level, the variations on the measured amplitude and phase signals are used, herein, to sense morphological cell changes in real time.

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Mechano-optical effects in multiwall carbon nanotubes ethanol based nanofluids

García-Merino¹,

Mercado-Zúñiga²,

Miguel³

et al. 2018

Opt. Express

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A highly sensitive technique for analyzing surface tension and dynamic viscosity of nanofluids was reported. Multiwall carbon nanotubes suspended in ethanol were evaluated. The assistance of a Fabry-Perot interferometer integrated by a small sample volume fluid allowed us to explore the stability and mechanical properties exhibited by the nanostructures. The surface tension and dynamic viscosity of the colloid was examined by using interferometric optical signals reflected from a remnant drop pending at the end of an optical fiber. Nanosecond pulses provided by a Nd:YAG laser source with 9.5 MW/mm at 532 nm wavelength were used to induce mechano-optical effects in the liquid drop. The mechanical parameters were approximated, taking into account single optical pulses interacting with an inelastic mass-spring-damper system.

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Flow characterization and patch clamp dose responses using jet microfluidics in a tubeless microfluidic device

Cited by 3 publications

References 33 publications

Advances in microfluidic in vitro systems for neurological disease modeling

Advances in microfluidic in vitro systems for neurological disease modeling

Label-free single-cell counting and characterization in the GHz-range

Mechano-optical effects in multiwall carbon nanotubes ethanol based nanofluids

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