Flow cytometric analysis of conjunctival epithelium in ocular rosacea and keratoconjunctivitis sicca

Pisella, Pierre‐Jean; Brignole, F.; Debbasch, C.; Lozato, P; Creuzot‐Garcher, Catherine; Bara, Jacques; Saïag, Philippe; Warnet, Jean‐Michel; Baudouin, Christophe

doi:10.1016/s0161-6420(00)00347-x

Cited by 146 publications

(104 citation statements)

References 22 publications

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“…Mean age of the CF group was 14.8274.11 years (range 7-23) vs 15.5174.65 years (range [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] in the control group. Only subjects with absolutely normal criteria and not having received any eye drops for at least 3 months were used for normal population analyses.…”

Section: Methodsmentioning

confidence: 99%

“…9 It is known that inflammation plays an essential role in the pathogenesis of dry eye. [9][10][11][12][13][14][15] The human conjunctival epithelium is a nonkeratinized squamous tissue composed of three to five layers that cover 80% of the ocular surface. It has a complex arrangement of microvilli and microplicae and contains goblet cells, Langerhans cells, some lymphocytes, and melanocytes.…”

Section: Introductionmentioning

confidence: 99%

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Flow cytometric analysis of HLA-DR antigen in conjunctival epithelial cells of patients with cystic fibrosis

Mrugacz

Zak

Bakunowicz-Łazarczyk

et al. 2006

Eye

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Purpose Cystic fibrosis (CF) is an autosomal-recessive genetic disorder. The disease affects all secretory epithelia including the eye and belongs to the group of ocular surface epithelial diseases, termed keratoconjunctivitis sicca that develop in dry eye. In the pathogenesis of dry eye, inflammation plays a crucial role. The aim of this study was to investigate the expression of HLA-DR on conjunctival epithelial cells from patients with CF. Materials and methods Twenty-five patients with CF and 25 normal subjects underwent ocular examination. Tear film break-up time (TBUT), Schirmer test, lissamine green staining, and conjunctival impression cytology were carried out. Cells were processed for flow cytometry, by using monoclonal antibodies to HLA-DR. Results The Schirmer test and TBUT scores were significantly lower in CF patients compared with controls. A significant increase of HLA-DR expression on epithelial cells was found in patients with CF compared with normal eyes. The Schirmer and TBUT test were positively correlated with HLA-DR expression for the percentage of cells. Conclusion These results suggest that conjunctival epithelial cells play an important proinflammatory role in ocular changes in CF patients. Our findings confirm the presence of an inflammatory background and the immune nature of this disease. HLA-DR measurement might be a useful method for monitoring of inflammatory processes in the conjunctiva and could be helpful in the use of antiinflammatory drugs in the treatment of ocular findings in CF patients.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Flow cytometric analysis of HLA-DR antigen in conjunctival epithelial cells of patients with cystic fibrosis

Mrugacz

Zak

Bakunowicz-Łazarczyk

et al. 2006

Eye

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“…Tumor necrosis factor (TNF-alfa) was also elevated in rosacea (23) . Significant overexpression of ICAM-1 (intercellular adhesion molecule 1) and HLA-DR, inflammatory markers, was observed in conjunctival epithelial cells of patients with rosacea (24) .…”

Section: Etiology and Pathophysiologymentioning

confidence: 99%

“…Recurrent hordeolum/chalazion and tear film insufficiency are commonly observed as a consequence of meibomian gland dysfunction (9,15) . Dry eyes, with abnormal Schirmer test, were reported in 56-62.5% of patients with ocular rosacea (24,51,63) . Diminished tear break-up time (TBUT) has also been reported in a large majority of patients with ocular rosacea (24,51,60) .…”

Section: Eyelids and Tear Filmmentioning

confidence: 99%

Ocular rosacea: a review

Vieira¹,

Höfling-Lima²,

Mannis³

2012

Arq. Bras. Oftalmol.

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“…Studies performed on impression cytology with FCM techniques confirmed an inflammatory pattern in a large number of patients with dry eye. [16][17][18][19] The purpose of this study was to assess the effect of HP-Guar added to conventional post-phacoemulsification treatment in dry eye signs and symptoms, and its influence on the expression of various inflammatory markers by FCM in impression cytology specimens.…”

Section: Introductionmentioning

confidence: 99%

The effect of preservative-free HP-Guar on dry eye after phacoemulsification: a flow cytometric study

Sánchez

Arriola‐Villalobos

Torralbo-Jiménez³

et al. 2010

Eye

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Purpose To assess the effect of hydroxypropyl (HP)-Guar added to regular post-phacoemulsification treatment in dry eye signs and symptoms, and its influence on the expression of various inflammatory markers by flow cytometry (FCM) in impression cytology specimens. Methods This prospective, interventional, single-centre study included 48 eyes of 48 patients with age-related cataract. After phacoemulsification, patients were randomised to the usual treatment group (UT), with 21 patients who received tobramycin and dexamethasone eye drops (Tobradex, Alcon Cusí, Spain), and the HP-Guar group, with 27 patients who received the UT plus preservative-free artificial tears (Systane UD, Alcon Cusí, Spain). Corneal and conjunctival staining with fluorescein and lissamine green, tear film break-up time (TBUT), Schirmer's I test with anaesthesia (Jones test), tear clearance, and ocular surface disease index (OSDI) were assessed preoperatively and 1 month after surgery. Besides, conjunctival impression cytology was performed in order to investigate inflammatory markers (CD3, CD11b, and HLA-DR) using FCM.

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Flow cytometric analysis of conjunctival epithelium in ocular rosacea and keratoconjunctivitis sicca

Cited by 146 publications

References 22 publications

Flow cytometric analysis of HLA-DR antigen in conjunctival epithelial cells of patients with cystic fibrosis

Flow cytometric analysis of HLA-DR antigen in conjunctival epithelial cells of patients with cystic fibrosis

Ocular rosacea: a review

The effect of preservative-free HP-Guar on dry eye after phacoemulsification: a flow cytometric study

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