Flow cytometric analysis of isolated rat liver nuclei during growth

Papa, Stefano; Capitani, Silvano; Matteucci, Alessandro; Vitale, Marco; Martelli, Alberto M.; Maraldi, Nadir M.; Manzoli, Francesco A.

doi:10.1002/cyto.990080611

Cited by 17 publications

(8 citation statements)

References 16 publications

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“…The purity of the parenchymal cells was determined by flow cytometry to be more than 95%. 16 The medium was replaced with fresh serum‐free Williams’ E medium, and the cultured cells were further incubated with hepatotoxin or cytokines for an additional 20 h.…”

Section: Methodsmentioning

confidence: 99%

Expression of hepatic thrombopoietin mRNA in primary cultured hepatocytes and in rats with acute liver injury or bone marrow suppression with or without cirrhosis

Ishikawa

Ichida

Matsuda

et al. 2000

J of Gastro and Hepatol

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Our results suggest that bone marrow is not a regulator of hepatic TPO production in cirrhosis. The reduced TPO mRNA expression found in cirrhotic rats may not result merely from serious cellular damage; it may be associated with cirrhosis-specific regulatory mechanisms for the expression of the TPO gene. Further studies are needed to search for other factors that may induce reduced TPO expression.

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Section: Methodsmentioning

confidence: 99%

Expression of hepatic thrombopoietin mRNA in primary cultured hepatocytes and in rats with acute liver injury or bone marrow suppression with or without cirrhosis

Ishikawa

Ichida

Matsuda

et al. 2000

J of Gastro and Hepatol

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“…nuclear ploidy) were estimated from the intensity of orange PI fluorescence. The degree of apoptosis was estimated from the percentage of nuclei with a DNA content lower than the diploid (2C) peak in a single-parameter histogram of PI fluorescence distribution (24)(25)(26)(27).…”

Section: Flow Cytometric Analysis Of Dna Fragmentationmentioning

confidence: 99%

Cytotoxic and Apoptotic Effects on Hepatocytes of Secondary Metabolites Obtained from Lichens

et al. 2004

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There are a large number of species of Antarctic lichens, and several studies describing the secondary metabolites present in these lichens, as well as the advances in understanding the chemistry of these metabolites, have been reported. In addition, some derivatives displaying interesting antibacterial effects have been described. The cytotoxic and apoptotic effects of 15 secondary metabolites (depsides, depsidones and usnic acid) obtained from Continental (Chilean) and Antarctic lichens were evaluated in primary cultures of rat hepatocytes. Intracellular lactate dehydrogenase release, caspase 3 activation and DNA fragmentation were measured. In this study, we have evaluated a set of markers associated with pivotal steps in the execution phase of apoptosis, in order to detect compounds with apoptotic effects on hepatocytes before significant necrosis takes place. Flow cytometric analysis of DNA fragmentation revealed an increase in apoptotic nuclei with sub-diploid DNA content after the exposure of hepatocytes to sub-cytotoxic concentrations of the compounds. Among these, salazinic acid, stictic acid and psoromic acid displayed significant apoptotic activities. Divaricatic acid showed only moderate apoptotic effects at sub-cytotoxic concentrations.

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“…Nuclei from woodchuck hepatocytes differ from those of rats and mice because they maintain diploid DNA content from infancy into adulthood (9,10,(32)(33)(34)(35). Virtually all strains of rats and mice are characterized by prominent, age-related onset of polyploidization of hepatocellular nuclei that begins early in life.…”

Section: Discussionmentioning

confidence: 99%

Nuclear ploidy of normal and neoplastic hepatocytes from woodchuck hepatitis virus-infected and uninfected woodchucks

Cullen¹

1994

Hepatology

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Flow cytometric analysis of the ploidy of normal and neoplastic hepatocyte nuclei obtained from adult woodchucks, a model of human hepadnavirus-induced hepatocellular carcinoma, was performed. All 36 samples of nuclei from non-neoplastic liver from woodchuck hepatitis virus-infected or uninfected liver were diploid, indicating that age-related nuclear polyploidization does not occur in this species, unlike other rodents. Individual or multiple hepatic neoplasms were obtained from each of 14 woodchuck hepatitis virus-infected woodchucks. Nineteen samples of hepatocellular carcinoma and eight adenomas were examined. Aneuploid nuclei were detected in 10 of the hepatocellular carcinomas and three of the adenoma samples. Similar DNA indexes, ranging from 1.11 to 1.22, were found in 7 of the 10 aneuploid HCCs and all 3 aneuploid adenomas. Nine of the 19 hepatocellular carcinoma samples and 5 of the 8 adenomas were diploid. Four of the diploid hepatocellular carcinomas had increased proportions of tetraploid nuclei. The presence of aneuploid nuclei was not related to histological appearance of the neoplasms or serum gamma-glutamyltranspeptidase levels. Because none of the hepatocellular carcinomas metastasized, the presence of aneuploidy could not be related to biological behavior. We determined the proportion of uninucleate and binucleate hepatocytes in hepatocellular carcinoma and nonneoplastic liver. Approximately 7% of hepatocytes were binucleate in nonneoplastic liver from woodchuck hepatitis virus-infected and uninfected liver. Only 2% of malignant hepatocytes were binucleate. The results of this study indicate that aneuploidy is a common change in hepatic neoplasms from woodchuck hepatitis virus-infected woodchucks.

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Flow cytometric analysis of isolated rat liver nuclei during growth

Cited by 17 publications

References 16 publications

Expression of hepatic thrombopoietin mRNA in primary cultured hepatocytes and in rats with acute liver injury or bone marrow suppression with or without cirrhosis

Expression of hepatic thrombopoietin mRNA in primary cultured hepatocytes and in rats with acute liver injury or bone marrow suppression with or without cirrhosis

Cytotoxic and Apoptotic Effects on Hepatocytes of Secondary Metabolites Obtained from Lichens

Nuclear ploidy of normal and neoplastic hepatocytes from woodchuck hepatitis virus-infected and uninfected woodchucks

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