Flow Cytometric Analysis of Leukocytes in the Human Female Reproductive Tract: Comparison of Fallopian Tube, Uterus, Cervix, and Vagina

Givan, Alice L.; White, Hillary D.; Stern, Joshua; Colby, E D; Gosselin, Edmund J.; Guyre, Paul M.; Wira, Charles R.

doi:10.1111/j.1600-0897.1997.tb00311.x

Cited by 234 publications

(271 citation statements)

References 29 publications

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“…NK cells appear during the proliferative phase and increase in cell numbers during the secretory phase of the menstrual cycle (12)(13)(14). A portion of this expansion is most likely due to proliferation of NK cells, because some are found in late secretory phase that express the Ki-67 Ag indicative of cell proliferation (29).…”

Section: Discussionmentioning

confidence: 99%

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Recruitment of Uterine NK Cells: Induction of CXC Chemokine Ligands 10 and 11 in Human Endometrium by Estradiol and Progesterone

Sentman

Meadows

Wira³

et al. 2004

The Journal of Immunology

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Uterine NK (uNK) cells express a unique set of markers compared with blood NK cells. However, recent studies suggest that uNK cells may be derived from the recruitment of blood NK cells into the endometrium. In this study, we used an in vitro organ culture system to demonstrate that estradiol induces expression of chemokines CXCL10 and/or CXCL11 within human endometrium in 85% of patient samples tested. The average increase in gene expression after 10−9 M estradiol treatment was 8.5-fold for CXCL10 and 7.7-fold for CXCL11 compared with medium alone. We observed that a specific estrogen receptor antagonist (ICI182780) was able to prevent chemokine gene induction, indicating that the effect of estradiol was receptor mediated. Moreover, our study showed that progesterone induced CXCL10 and CXCL11 expression in 83% of endometrial samples tested. We have also found that uNK cells and blood NK cells express the receptor for CXCL10 and CXCL11, CXCR3, with the highest expression found on uNK cells and CD56bright blood NK cells. These data indicate that sex hormones induce specific chemokines in nonpregnant human endometrium that can activate NK cell migration, and suggest that this mechanism may account for the increased NK cell numbers in endometrium during the menstrual cycle.

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Section: Discussionmentioning

confidence: 99%

“…The interplay between these different cell types and their roles in defense against pathogen invasion in this specialized tissue are poorly understood. Unlike in the murine uterus, uNK cells in the human uterus are found widely dispersed throughout the EM, and their numbers increase as the menstrual cycle progresses (12)(13)(14).…”

mentioning

confidence: 99%

Recruitment of Uterine NK Cells: Induction of CXC Chemokine Ligands 10 and 11 in Human Endometrium by Estradiol and Progesterone

Sentman

Meadows

Wira³

et al. 2004

The Journal of Immunology

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146

110

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“…With menopause, sex hormone fluctuations decline and hormone levels remain low and constant (Givan et al., 1997; Wira, Rodriguez‐Garcia & Patel, 2015). Sex hormones regulate immune cell populations and function in the FRT throughout the menstrual cycle to prepare for implantation and pregnancy (Wira et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

Aging impacts CD103⁺CD8⁺ T cell presence and induction by dendritic cells in the genital tract

et al. 2018

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SummaryAs women age, susceptibility to systemic and genital infections increases. Tissue‐resident memory T cells (TRMs) are CD103+ CD8+ long‐lived lymphocytes that provide critical mucosal immune protection. Mucosal dendritic cells (DCs) are known to induce CD103 expression on CD8+ T cells. While CD103+ CD8+ T cells are found throughout the female reproductive tract (FRT), the extent to which aging impacts their presence and induction by DCs remains unknown. Using hysterectomy tissues, we found that endometrial CD103+ CD8+ T cells were increased in postmenopausal compared to premenopausal women. Endometrial DCs from postmenopausal women were significantly more effective at inducing CD103 expression on allogeneic naïve CD8+ T cells than DCs from premenopausal women; CD103 upregulation was mediated through membrane‐bound TGFβ signaling. In contrast, cervical CD103+ T cells and DC numbers declined in postmenopausal women with age. Decreases in DCs correlated with decreased CD103+ T cells in endocervix, but not ectocervix. Our findings demonstrate a previously unrecognized compartmentalization of TRMs in the FRT of postmenopausal women, with loss of TRMs and DCs in the cervix with aging, and increased TRMs and DC induction capacity in the endometrium. These findings are relevant to understanding immune protection in the FRT and to the design of vaccines for women of all ages.

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“…For instance, epithelia and stromal cells of the reproductive organs of the mouse and human, express high levels of TGF-1 and other molecules involved in conditioning immune privilege sites (Chegini et al, 1994;Grant and Wira, 2003;Jin et al, 1992;Wada et al, 1996). Moreover, antigen presenting cells such as macrophages and DCs, regularly present in the reproductive tissues might also contribute to regulatory response, since they could promote T regulatory cells differentiation trough production of IL-10 and TGF-1 after infection (Givan et al, 1997;Stagg et al, 1998).…”

Section: Mechanisms Of Immune Evasion By Neisseria Gonorrhoeaementioning

confidence: 99%

Mucosal Immunity and Evasion Strategies of Neisseria gonorrhoeae

Imarai¹,

Acuña²,

Escobar³

et al. 2012

Sexually Transmitted Infections

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Flow Cytometric Analysis of Leukocytes in the Human Female Reproductive Tract: Comparison of Fallopian Tube, Uterus, Cervix, and Vagina

Cited by 234 publications

References 29 publications

Recruitment of Uterine NK Cells: Induction of CXC Chemokine Ligands 10 and 11 in Human Endometrium by Estradiol and Progesterone

Recruitment of Uterine NK Cells: Induction of CXC Chemokine Ligands 10 and 11 in Human Endometrium by Estradiol and Progesterone

Aging impacts CD103⁺CD8⁺ T cell presence and induction by dendritic cells in the genital tract

Mucosal Immunity and Evasion Strategies of Neisseria gonorrhoeae

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Flow Cytometric Analysis of Leukocytes in the Human Female Reproductive Tract: Comparison of Fallopian Tube, Uterus, Cervix, and Vagina

Cited by 234 publications

References 29 publications

Recruitment of Uterine NK Cells: Induction of CXC Chemokine Ligands 10 and 11 in Human Endometrium by Estradiol and Progesterone

Recruitment of Uterine NK Cells: Induction of CXC Chemokine Ligands 10 and 11 in Human Endometrium by Estradiol and Progesterone

Aging impacts CD103+CD8+ T cell presence and induction by dendritic cells in the genital tract

Mucosal Immunity and Evasion Strategies of Neisseria gonorrhoeae

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Product

Resources

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Aging impacts CD103⁺CD8⁺ T cell presence and induction by dendritic cells in the genital tract