Flow cytometric analysis of the cadmium-exposed green alga<i>Chlamydomonas reinhardtii</i>(Chlorophyceae)

Jamers, An; Lenjou, Marc; Deraedt, Pieter; Bockstaele, Dirk Van; Blust, Ronny; Coen, Wim De

doi:10.1080/09670260903118214

Cited by 77 publications

(41 citation statements)

References 41 publications

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“…In the present work, algal autofluorescence was found to be enhanced in cells exposed to intermediate and high concentrations of Cr(VI) and Cu(II). Similarly, an increase in Chl a intensity in C. reinhardtii exposed to Cd for 48 h was reported (Jamers et al 2009). Franklin et al (2001b) reported that Cu(II) can cause both an increase and a decrease in the Chl a fluorescence of Chlorella sp., P. subcapitata and Phaeodactylum tricornutum, depending of the time of exposure and the Cu(II) concentration.…”

Section: Discussionsupporting

confidence: 54%

Use of a fluorescence-based approach to assess short-term responses of the alga Pseudokirchneriella subcapitata to metal stress

Machado

Soares

2014

J Appl Phycol

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This work explores the use of fluorescent probes to evaluate the responses of the green alga Pseudokirchneriella subcapitata to the action of three nominal concentrations of Cd(II), Cr(VI), Cu(II) and Zn(II) for a short time (6 h). The toxic effect of the metals on algal cells was monitored using the fluorochromes SYTOX Green (SG, membrane integrity), fluorescein diacetate (FDA, esterase activity) and rhodamine 123 (Rh123, mitochondrial membrane potential). The impact of metals on chlorophyll a (Chl a) autofluorescence was also evaluated. Esterase activity was the most sensitive parameter. At the concentrations studied, all metals induced the loss of esterase activity. SG could be used to effectively detect the loss of membrane integrity in algal cells exposed to 0.32 or 1.3 μmol L −1 Cu(II). Rh123 revealed a decrease in the mitochondrial membrane potential of algal cells exposed to 0.32 and 1.3 μmol L −1 Cu(II), indicating that mitochondrial activity was compromised. Chl a autofluorescence was also affected by the presence of Cr(VI) and Cu(II), suggesting perturbation of photosynthesis. In conclusion, the fluorescence-based approach was useful for detecting the disturbance of specific cellular characteristics. Fluorescent probes are a useful diagnostic tool for the assessment of the impact of toxicants on specific targets of P. subcapitata algal cells.

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Section: Discussionsupporting

confidence: 54%

Use of a fluorescence-based approach to assess short-term responses of the alga Pseudokirchneriella subcapitata to metal stress

Machado

Soares

2014

J Appl Phycol

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“…After pollutant treatment (e.g., Cu; nickel, Ni), the cell size of algal cells Chlorococcum infusionum was reduced during 6 days of culture (Nam and An 2015) and the cell size was far more sensitive than other parameters of algal viability such as cell granularity and autofluorescence of chlorophyll. Similarly, Jamers et al (2009) reported that Chlamydomonas reinhardtii exhibited a decline in cell size under 5 and 100 mM Cd exposure during 72 h of culture. The decrease in cell volume in response to Pb may be connected with toxic effect of heavy metals on the structure of cell wall and the leakage of ions or cell components through plasma membrane leading to irreversible cell damage and necrosis (Jamers et al 2009;Nam and An 2015).…”

Section: Discussionmentioning

confidence: 72%

The effect of lead on the growth, content of primary metabolites, and antioxidant response of green alga Acutodesmus obliquus (Chlorophyceae)

Piotrowska‐Niczyporuk

Bajguz

Talarek

et al. 2015

Environ Sci Pollut Res

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Green unicellular alga Acutodesmus obliquus (Turpin) Hegewald et Hanagata (SAG strain no. 276-6) (Chlorophyceae) was used for determination of phytotoxicity of lead (Pb) at the range of concentrations 0.01-500 μM during 7 days of culture. The accumulation of Pb in algal cells was found to be increased in a concentration- and duration-dependent manner. The highest Pb uptake value was obtained in response to 500 μM Pb on the seventh day of cultivation. The decrease in the number and the size of cells and the contents of selected primary metabolites (photosynthetic pigments, monosaccharides, and proteins) in A. obliquus cells were observed under Pb stress. Heavy metal stimulated also formation of reactive oxygen species (hydrogen peroxide) and oxidative damage as evidenced by increased lipid peroxidation. On the other hand, the deleterious effects of Pb resulting from the cellular oxidative state can be alleviated by enzymatic (superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase) and non-enzymatic (ascorbate, glutathione) antioxidant systems. These results suggest that A. obliquus is a promising bioindicator of heavy metal toxicity in aquatic environment, and it has been identified as good scavenger of Pb from aqueous solution.

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“…In particular, esterases comprise a key group of ubiquitous enzymes in plants and animals which have been shown to relate well to metabolic activity and cell viability (Dorsey et al 1989;Gala and Giesy 1990). Esterase activity has been used as a sensitive endpoint in different toxicity tests with microalgae, proving to be a reliable indicator of the toxic effects of pollutants (Hadjoudja et al 2009;Jamers et al 2009;Lage et al 2001;Yu et al 2007). A decline in enzyme activity is usually considered indicative of the presence of stress (Franklin et al 2001;Regel et al 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, fluorescent probes have been applied to physiological studies of algae and other microorganisms (Adler et al 2007;Rioboo et al 2009;Veal et al 2000). In an ecotoxicological context, there are recent studies with microalgae as test organisms in which it was demonstrated that, although very rarely used in ecotoxicology, FCM combined with the use of different fluorochromes is a quick and convenient technique to assess toxic effects of pollutants that can generate information on the mode of action of contaminants (Adler et al 2007;Jamers et al 2009). However, the potential of this technique as a tool in microalgal toxicity bioassays has not yet been fully exploited.…”

Section: Introductionmentioning

confidence: 99%

Flow cytometric analysis to evaluate physiological alterations in herbicide-exposed Chlamydomonas moewusii cells

et al. 2011

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Investigation of herbicide toxicology in non-target aquatic primary producers such as microalgae is of great importance from an ecological point of view. In order to study the toxicity of the widely used herbicide paraquat on freshwater green microalga Chlamydomonas moewusii, physiological changes associated with 96 h-exposures to this pollutant were monitored using flow cytometry (FCM) technique. Intracellular reactive oxygen species concentration, cytoplasmic membrane potential, metabolic activity and cell protein content were monitored to evaluate the toxicological impact of paraquat on algal physiology. Results showed that herbicide paraquat induced oxidative stress in C. moewusii cells, as it indicated the increase of both superoxide anion and hydrogen peroxide levels observed in non-chlorotic cells of cultures exposed to increasing herbicide concentrations. Furthermore, a progressive increase in the percentage of depolarised cells and a decrease in the metabolic activity level were observed in response to paraquat when non-chlorotic cells were analysed. Chlorotic cells were probably non-viable cells, based on the cytoplasmic membrane depolarisation, its metabolically non-active state and its drastically reduced protein content. In view of the obtained results, we have concluded that a range of significant physiological alterations, detected by flow cytometry, occur when C. moewusii, an ubiquitous microalga in freshwater environments, is challenged with environmentally relevant concentrations of the herbicide paraquat.

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Flow cytometric analysis of the cadmium-exposed green algaChlamydomonas reinhardtii(Chlorophyceae)

Cited by 77 publications

References 41 publications

Use of a fluorescence-based approach to assess short-term responses of the alga Pseudokirchneriella subcapitata to metal stress

Use of a fluorescence-based approach to assess short-term responses of the alga Pseudokirchneriella subcapitata to metal stress

The effect of lead on the growth, content of primary metabolites, and antioxidant response of green alga Acutodesmus obliquus (Chlorophyceae)

Flow cytometric analysis to evaluate physiological alterations in herbicide-exposed Chlamydomonas moewusii cells

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