Flow cytometric analysis of the V? repertoire in healthy controls

Beemd, René van den; Boor, Patrick P.C.; Lochem, Ellen G. van; Hop, Wim C. J.; Langerak, Anton W.; Wolvers-Tettero, I. L. M.; Hooijkaas, Herbert; Dongen, Jacques J. M. van

doi:10.1002/1097-0320(20000801)40:4<336::aid-cyto9>3.0.co;2-0

Cited by 165 publications

(124 citation statements)

References 47 publications

Supporting

Mentioning

111

Contrasting

Unclassified

Order By: Relevance

“…30,67 The development of monoclonal antibodies against most Vb domains has helped to identify Vb family expansions. 13 However, TCR gene rearrangement analysis is essential for clonality assessment in T-cell lymphoproliferative disorders. As the restricted germline-encoded repertoire of the TCRG and TCRD loci facilitates DNA-based PCR approaches, various PCR methods have been established for the detection of TCRG and TCRD gene rearrangements.…”

Section: Resultsmentioning

confidence: 98%

“…Mean values of the Vb repertoire seem to be stable during aging, although the standard deviation increase in the elderly. 13,92 Also in the human thymus some Vb gene segments dominate: the most prevalent seven Vb genes (Vb3-1, Vb4-1, Vb5-1, Vb6-7, Vb7-2, Vb8-2, and Vb13-2) cover nearly half of the entire functional TCRB repertoire. 93 The representation of J segments is also far from even.…”

Section: Resultsmentioning

confidence: 99%

“…[11][12][13][14][15][16] In the interpretation of monotypic Vb results using 20-25 antibodies against different Vb families (Table 2), one should realize that 1 Jaffe et al, 2 and van Dongen et al 5 …”

Section: Ig and Tcr Repertoirementioning

confidence: 99%

“…13,17 These clonal T-cell expansions in PB are, however, relatively small in size: o40% of PB T-lymphocytes and o0.5 Â 10 6 /ml PB. 13 It is not yet clear to what extent such clinically benign T-cell clones can also be found in lymphoid tissues.…”

Section: Figurementioning

confidence: 99%

See 3 more Smart Citations

Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerted Action BMH4-CT98-3936

et al. 2003

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Ig and Tcr Repertoirementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

See 2 more Smart Citations

Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerted Action BMH4-CT98-3936

et al. 2003

View full text Add to dashboard Cite

“…This panel of antibodies covers B70% of the T-cell receptor-Vb repertoires of normal T cells. 31 In most cases, CD3-APC and CD4-PerCP-Cy5.5 were included in each tube for gating. Flow cytometric Vb analysis was performed primarily on CD3 þ CD4 þ T cells.…”

Section: Flow Cytometric Immunophenotyping and Analysis Of T-cell Recmentioning

confidence: 99%

Flow cytometric detection of peripheral blood involvement by mycosis fungoides and Sézary syndrome using T-cell receptor Vβ chain antibodies and its application in blood staging

et al. 2010

View full text Add to dashboard Cite

Peripheral blood involvement has been recognized as an adverse prognostic factor in patients with mycosis fungoides and Sé zary syndrome. However, accurate identification and enumeration of the neoplastic cells in these diseases can be challenging. We assessed the clinical utility of flow cytometric immunophenotypic analysis of T-cell receptor Vb expression in 82 mycosis fungoides and 6 Sé zary syndrome patients, with an atypical T-cell immunophenotype, or abnormal CD4:CD8 ratio, identified from peripheral blood specimens of 723 patients submitted for routine mycosis fungoides/Sé zary syndrome blood staging. To improve detection sensitivity, Vb expression was analyzed on gated CD3 þ CD4 þ T cells or T cells with an aberrant immunophenotype, if present. The flow cytometric results were compared with traditional morphologic assessment (n ¼ 88) and molecular methods to assess the T-cell receptor c or b genes (n ¼ 41 tested in parallel). Flow cytometric immunophenotyping yielded a clonal Vb pattern in 60/82 mycosis fungoides and 6/6 Sé zary syndrome patients. By contrast, flow cytometric Vb was negative in all 10 healthy donors and 18 control patients, showing a specificity of 100% and concordance with molecular testing of 86%. Using flow cytometric Vb results instead of morphologic assessment, 12 patients were upstaged from B1 to B2, and 20 patients from B0 to B1 (Po0.0001). The 12 upstaged B2 patients had no morphologic evidence of involvement, but had an aggressive clinical course similar to those staged by traditional morphologic assessment (median survival 27 vs 41 months, log-rank P ¼ 0.701). In 30/44 patients with a tumor-associated Vb expression, a single Vb tube was used to monitor treatment response. In conclusion, flow cytometric Vb analysis is rapid and convenient, can assess T-cell clonality and tumor quantity simultaneously, and is useful both in initial blood staging and monitoring tumor burden during therapy in patients with mycosis fungoides or Sé zary syndrome.

show abstract

T-cell receptor analysis by flow cytometry

McCoy

2001

Cytometry

View full text Add to dashboard Cite

The synthesis of a 93‐residue chemokine, lymphotactin, containing eight sites of O‐linked glycosylation, was achieved using the technique of native chemical ligation. A single GalNAc residue was incorporated at each glycosylation site using standard Fmoc‐chemistry to achieve the first total synthesis of a mucin‐type glycoprotein. Using this approach quantities of homogeneous material were obtained for structural and functional analysis.

show abstract

Flow cytometric analysis of the V? repertoire in healthy controls

Cited by 165 publications

References 47 publications

Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerted Action BMH4-CT98-3936

Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerted Action BMH4-CT98-3936

Flow cytometric detection of peripheral blood involvement by mycosis fungoides and Sézary syndrome using T-cell receptor Vβ chain antibodies and its application in blood staging

T-cell receptor analysis by flow cytometry

Contact Info

Product

Resources

About