Flow cytometric analysis of transport activity in lymphocytes electroporated with a fluorescent organic anion dye

Dinchuk, Joseph; Kelley, Keith A.; Callahan, Gerald N.

doi:10.1016/0022-1759(92)90293-3

Cited by 15 publications

(8 citation statements)

References 30 publications

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“…Optimizing electroporation parameters is crucial for the future application and can be easily performed in cell suspension using, e.g., propidium iodide [38,39] or lucifer yellow [40]. The electric field intensity is the key parameter in electrochemotherapeutic protocols as it should lead to the pore formation without simultaneous cell destruction.…”

Section: Discussionmentioning

confidence: 99%

Doxorubicin Assisted by Microsecond Electroporation Promotes Irreparable Morphological Alternations in Sensitive and Resistant Human Breast Adenocarcinoma Cells

et al. 2020

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Electroporation increases the transmembrane transport of molecules. The combination of electric pulses with cytostatic compounds is beneficial for cancer treatment. Doxorubicin (DOX) is a commonly used chemotherapeutic anticancer drug. Its fluorescence properties enable the investigation of drug distribution and metabolism. In this study, doxorubicin was enhanced by electroporation to eliminate cancer cells more effectively. The influence of electroporation on the drug uptake was evaluated in two cell lines: MCF-7/WT and MCF-7/DOX. The intracellular localization of doxorubicin and its impact on the intracellular structure organization were examined under a confocal microscope. Cellular effects were examined with the 3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test that estimates the rate of metabolism in viable cells. The ultrastructure (TEM) of tumor cells subjected to the electric field was analyzed. An enhanced doxorubicin efficacy was observed in MCF-7/DOX cells after combination with electroporation. The response of the resistant cell line was revealed to be more sensitive to electric pulses. Electroporation-based methods may be attractive for cancer treatment in human breast adenocarcinoma, especially with acquired resistance. Electroporation enables a reduction of the effective dose of the drugs and the exposure time in this type of cancer, diminishing side effects of the systemic therapy.

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Section: Discussionmentioning

confidence: 99%

Doxorubicin Assisted by Microsecond Electroporation Promotes Irreparable Morphological Alternations in Sensitive and Resistant Human Breast Adenocarcinoma Cells

et al. 2020

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“…Electroporation was initially developed in the early 1980s to enable gene transfection of bacterial cells and mammalian cell cultures [16][17][18][19]. Its application was broadened to achieve enhanced uptake of ions, drugs [20,21], dyes [22,23], immunohistochemical tracers [24], DNA, genes and chemotherapeutic agents into cells (see [16] for a review of EPT fundamentals). The technique uses pulsed, brief, high-intensity electrical fields to temporarily increase cell membrane permeability.…”

Section: Electroporation Therapymentioning

confidence: 99%

Role of electrochemotherapy in the treatment of metastatic melanoma and other metastatic and primary skin tumors

Byrne¹,

Thompson²

2006

Expert Review of Anticancer Therapy

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Electroporation is a novel therapeutic modality that uses pulsed electrical currents to enhance the uptake of drugs, vaccines and genes into cells, and has been used for over 20 years. Electroporation therapy using cytotoxic drugs is called electrochemotherapy. Electrochemotherapy has been studied in vitro, in vivo and in clinical trials. It is potentially useful for treating patients with metastatic tumors, such as melanoma, and even select primary tumors, such as head and neck squamous cell carcinomas and basal cell carcinoma. Various chemotherapeutic agents have been tested with electroporation therapy, but bleomycin and cisplatin are the two most widely used. The biological basis of electroporation therapy is outlined in this review and basic science studies and the limited clinical studies that have involved electrochemotherapy are reviewed. Particular focus is placed on trials involving melanoma, head and neck cancers and other primary and metastatic skin cancers.

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“…Consequently, electroporation has been used to introduce ions, dyes (Mir et al . 1988, Dinchuk et al . 1992, Gehl et al .…”

Section: The Process Of Electroporationmentioning

confidence: 99%

“…1995, Gabriel & Teissie 1997) or lucifer yellow (Mir et al . 1988, Dinchuk et al . 1992), and by using, e.g.…”

Section: Optimization Of In Vitro Electroporation Protocolsmentioning

confidence: 99%

Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research

Gehl

2003

Acta Physiologica Scandinavica

797

555

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Electroporation designates the use of short high-voltage pulses to overcome the barrier of the cell membrane. By applying an external electric field, which just surpasses the capacitance of the cell membrane, transient and reversible breakdown of the membrane can be induced. This transient, permeabilized state can be used to load cells with a variety of different molecules, either through simple diffusion in the case of small molecules, or through electrophoretically driven processes allowing passage through the destabilized membrane--as is the case for DNA transfer. Initially developed for gene transfer, electroporation is now in use for delivery of a large variety of molecules: From ions to drugs, dyes, tracers, antibodies, and oligonucleotides to RNA and DNA. Electroporation has proven useful both in vitro, in vivo and in patients, where drug delivery to malignant tumours has been performed. Whereas initial electroporation procedures caused considerable cell damage, developments over the past decades have led to sophistication of equipment and optimization of protocols. The electroporation procedures used in many laboratories could be optimized with limited effort. This review (i) outlines the theory of electroporation, (ii) discusses factors of importance for optimization of electroporation protocols for mammalian cells, (iii) addresses particular concerns when using electroporation in vivo, e.g. effects on blood flow and considerations regarding choice of electrodes, (iv) describes DNA electrotransfer with emphasis on use in the in vivo setting, and (v) sums up data on safety and efficacy of electroporation used to enhance delivery of chemotherapy to tumours in cancer patients.

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Flow cytometric analysis of transport activity in lymphocytes electroporated with a fluorescent organic anion dye

Cited by 15 publications

References 30 publications

Doxorubicin Assisted by Microsecond Electroporation Promotes Irreparable Morphological Alternations in Sensitive and Resistant Human Breast Adenocarcinoma Cells

Doxorubicin Assisted by Microsecond Electroporation Promotes Irreparable Morphological Alternations in Sensitive and Resistant Human Breast Adenocarcinoma Cells

Role of electrochemotherapy in the treatment of metastatic melanoma and other metastatic and primary skin tumors

Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research

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