Flow cytometric analysis of tumour‐infiltrating lymphocytes in patients with renal cell carcinoma

Kowalczyk, Dariusz W.; Skorupski, Witold; Kwias, Zbigniew; Nowak, Jerzy

doi:10.1046/j.1464-410x.1997.00408.x

Cited by 48 publications

(30 citation statements)

References 5 publications

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“…In up to 70 % of renal tumours, CD8 + T lymphocytes are the main cellular population of TIL cells (16). Our observations comply with this finding.…”

Section: Discussionsupporting

confidence: 92%

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Phenotype Analysis of Tumour-infiltrating Lymphocytes and Lymphocytes in Peripheral Blood in Patients with Renal Carcinoma

Kopecký

Lukesová

Vroblova

et al. 2007

Acta Med. (Hradec Kralove, Czech Repub.)

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Introduction: When checking tumour growth, a number of observations indicate that the immune system plays a significant role in patients with renal cell carcinoma (RCC). Infiltration by lymphocytes (tumour infiltrating lymphocytes, TILs) is more prevalent in RCC than any other tumours. T lymphocytes are the dominant population of TIL cells. Views concerning the role of T lymphocytic subpopulations, B lymphocytes and NK cells in an anti-tumour response are not established. Aim: The aim is to determine the phenotype and activation of T and B lymphocytic subpopulations and NK cells and to compare their representation in tumour stroma and peripheral blood lymphocytes (PBL) in patients with RCC. Material and methods: Samples of peripheral blood taken from the cubital and renal veins and tumour stroma cells were obtained from 44 patients in the course of their surgeries carried out due to primary RCC. TILs were isolated from mechanically disintegrated tumour tissue. Immunophenotype multiparametric analysis of PBL and TILs was carried out. Their surface and activation characteristics were determined by means of flow cytometer. Results: CD3+ T lymphocytes (69.7 %) were the main population of TILs. The number of CD3+/CD8+ T lymphocytes was significantly higher in TILs, 42.6 % (p< 0.01), while CD4+ T lymphocytes were the majority population in peripheral blood, 41.35 % (p < 0.001). The representation of CD3+/69+ T lymphocytes was significantly higher in TILs, 32.9 %, compared to PBL (p<0.001). On the contrary, the numbers of CD3+/CD25+, CD8+/57+ and CD4+/RA+ (naive CD4+ T lymphocytes) were higher in PBL (p<0.001). The differences in representation of (CD3-/16+56+) NK cells and CD3+/DR+ T cells in TILs and PBL were not significant. Conclusion: The above-mentioned results prove that the characteristics and intensity of anti-tumour responses are different in compared compartments (tumour/PBL). CD3+/CD8+ T lymphocytes are the dominant lymphocytic population of TILs. The knowledge of the phenotype and functions of effector cells, which are responsible for anti-tumour response, are the basic precondition for understanding the anti-tumour immune response and the cause of its failure.

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“…In up to 70 % of renal tumours, CD8 + T lymphocytes are the main cellular population of TIL cells (16). Our observations comply with this finding.…”

Section: Discussionsupporting

confidence: 92%

“…Th2 lymphocytes produce IL-13, which has a pro-angiogenic effect. Non-specific stimulation may be a consequence which is responsible for failure of immunological supervision (16,29).…”

Section: Discussionmentioning

confidence: 99%

Phenotype Analysis of Tumour-infiltrating Lymphocytes and Lymphocytes in Peripheral Blood in Patients with Renal Carcinoma

Kopecký

Lukesová

Vroblova

et al. 2007

Acta Med. (Hradec Kralove, Czech Repub.)

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“…In addition, the low TNF-α mRNA levels in the CD8 + TIL found in the present study indicate a possible anergy and low lytic capacity of this TIL subpopulation. This is in accordance with the finding that the composition of TIL depends on tumour grade, in as far as an increase in the percentage of CD8 + cells positively correlates with the tumour grade and bad prognosis (Igarashi et al, 1992;Kowalczyk et al, 1997).…”

Section: Discussionsupporting

confidence: 92%

Enhanced expression of IFN-γ mRNA in CD4+ or CD8+ tumour-infiltrating lymphocytes compared to peripheral lymphocytes in patients with renal cell cancer

et al. 2000

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SummaryThe mRNA expression of the cytokines IFN-γ, IL-10 and TNF-α and the proapoptotic factor Fas ligand (FasL) was compared in freshly isolated CD4 + and CD8 + tumour-infiltrating lymphocytes (TIL) and simultaneously obtained autologous CD4 + and CD8 + peripheral blood lymphocytes (PBL) from 20 patients with renal cell carcinomas (RCC). TIL were isolated from mechanically disaggregated tumour material and PBL from peripheral blood by gradient centrifugation. The cells of the interphase were depleted from tumour cells with antihuman epithelial antigen magnetic beads and then positive selection was performed with anti-CD4 or anti-CD8 magnetic beads. In these pure lymphocyte preparations the constitutive expression of cytokine and FasL mRNAs was determined by using a PCR-assisted mRNA amplification assay. In the CD4 + TIL from the 20 patients with RCC, levels of mRNAs encoding for IFN-γ (P ≤ 0.001), IL-10 (P ≤ 0.05), and FasL (P ≤ 0.001) were significantly higher than in the autologous CD4 + PBL. Comparison of CD8 + TIL and CD8 + PBL revealed a significant higher expression of IFN-γ (P ≤ 0.001), IL-10 (P ≤ 0.01) and FasL mRNAs (P ≤ 0.001) in the former. However, TNF-α mRNA levels were significantly lower in the CD8 + TIL than in the CD8 + PBL (P ≤ 0.05). These data reflect a general in vivo activation of RCC infiltrating lymphocytes in the tumour surrounding.

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“…A detailed understanding of the functional peculiarities of individual T lymphocyte subtypes may explain the paradox that the presence of TILs does not always correlate with improved prognosis and may also allow the development of targeted approaches that specifically augment antitumor immune responses. 17,[30][31][32][33][34][35][36][37][38] Antitumor functions of T lymphocytes…”

mentioning

confidence: 99%

Tumor-infiltrating T lymphocytes: friends or foes?

Yü

2006

Laboratory Investigation

254

173

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Flow cytometric analysis of tumour‐infiltrating lymphocytes in patients with renal cell carcinoma

Cited by 48 publications

References 5 publications

Phenotype Analysis of Tumour-infiltrating Lymphocytes and Lymphocytes in Peripheral Blood in Patients with Renal Carcinoma

Phenotype Analysis of Tumour-infiltrating Lymphocytes and Lymphocytes in Peripheral Blood in Patients with Renal Carcinoma

Enhanced expression of IFN-γ mRNA in CD4+ or CD8+ tumour-infiltrating lymphocytes compared to peripheral lymphocytes in patients with renal cell cancer

Tumor-infiltrating T lymphocytes: friends or foes?

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