Flow cytometric and cytogenetic analyses of Iberian Peninsula Festuca spp.

Loureiro, João; Kopecký, David; Castro, Sílvia; Santos, Conceição; Silveira, Paulo

doi:10.1007/s00606-007-0564-8

Cited by 50 publications

(46 citation statements)

References 34 publications

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“…In agreement with the previous study [Loureiro et al, 2007], histograms of relative DNA content contained well-resolved peaks representing nuclei in G 0 /G 1 and G 2 phases of the cell cycle ( fig. 1 ).…”

Section: Resultssupporting

confidence: 76%

“…The 1Cx value for F. arundinacea was 11% lower as compared to that of F. pratensis ( table 2 ). This difference could be due to the reduction in genome size known to take place following polyploidization [Leitch and Bennett, 2004], and which was also observed in Festuca [Seal, 1983;Loureiro et al, 2007]. On the other hand, the 1Cx values for both cultivars of L. perenne ( table 2 ) were statistically not different from the estimates for L. multiflorum with the same ploidy level.…”

Section: Resultsmentioning

confidence: 54%

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Physical Distribution of Homoeologous Recombination in Individual Chromosomes of Festuca pratensis in Lolium multiflorum

Kopecký

Havránková

Loureiro

et al. 2010

Cytogenet Genome Res

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Crossing over-based recombination is a powerful tool for generating new allelic combinations during sexual reproduction. It usually occurs between homologous chromosomes. However, under some conditions, homoeologues may also be capable of crossing over. Whether homologous and homoeologous crossovers are equivalent and governed by the same rules has never been established. Here we report on chromosome distribution of homoeologous crossovers in a unique system of Festuca × Lolium hybrids. Unlike in most other hybrids, in these intergeneric hybrids, homoeologous chromosomes are capable of pairing and crossing over with frequencies approaching that of homologues. At the same time, genome divergence makes cytological detection of chromosome recombination feasible. We analyzed the distribution of homoeologous recombination along individual chromosomes in a complete set of intergeneric single chromosome substitutions fromF. pratensisinto tetraploid L. multiflorum. Homoeologous recombination sites were not evenly distributed along the chromosomes, being concentrated in intercalary regions of the arms and reduced in proximal and distal regions. Several recombination hotspots and cold spots were found along individual chromosomes and the recombination was not affected by the presence of a secondary constriction. Our results indicate that despite the uneven distribution of homoeologous recombination, introgression of any part of the F. pratensis genome into L. multiflorum is feasible.

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Section: Resultssupporting

confidence: 76%

Section: Resultsmentioning

confidence: 54%

Physical Distribution of Homoeologous Recombination in Individual Chromosomes of Festuca pratensis in Lolium multiflorum

Kopecký

Havránková

Loureiro

et al. 2010

Cytogenet Genome Res

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“…Among the genera, Festuca is large and complex having more than 600 species with multiple ploidy levels ranging from diploid (2n = 2x = 14) up to dodecaploid (2n = 12x = 84) whereas Lolium is a small genus with 10 recognized diploid species (Clayton and Renvoize, 1986;Loureiro et al, 2007). The two genera, Festuca and Lolium include a number of important grasses used as pasture, fodder, and amenity purposes.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Relationships in the Festuca-Lolium Complex (Loliinae; Poaceae): New Insights from Chloroplast Sequences

et al. 2016

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The species within the Lolium/Festuca grass complex have dispersed and colonized large areas of temperate global grasslands both naturally and by human intervention. The species within this grass complex represent some of the most important grass species both for amenity and agricultural use worldwide. There has been renewed interest by grass breeders in producing hybrid combinations between these species and several countries now market Festulolium varieties as a combination of genes from both genera. The two genera have been differentiated by their inflorescence structure, but controversy has surrounded the taxonomic classification of the Lolium-Festuca complex species for several decades. In order to better understand the complexities within the Lolium/Festuca complex and their genetic background, the phylogeny of important examplers from the Lolium-Festuca complex were reconstructed. In total 40 taxa representing the Festuca and Lolium species with Vulpia myuros and Brachypodium distachyon as outgroups were sampled, using two non-coding intergenic spacers (trnQ-rps16, trnH-psbA) and one coding gene (rbcL). Maximum parsimony (MP), Bayesian inference (BI) analyses based on each partition and combined plastid DNA dataset, and median-jointing network analysis were employed. The outcomes strongly suggested that the subgen. Schedonorus has a close relationship to Lolium, and it is also proposed to move the sect. Leucopoa from subgen. Leucopoa to Subgen. Schedonorus and to separate sect. Breviaristatae from the subgen. Leucopoa. We found that F. californica could be a lineage of hybrid origin because of its intermediate placement between the "broad-leaved" and "fine-leaved" clade.

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“…Based on the distribution of rDNA loci, they considered F. scariosa a progenitor of F. mairei and F. glaucescens and F. altissima as good progenitor candidates for polyploid species of the Bovinae section. FISH with rDNA probes and GISH were also used for phylogenetic studies of the Iberian Peninsula fescues (Loureiro et al, 2007). Diploid species F. henriquesii was found to be a good candidate in evolution of F. ampla , as it had the same genomic distribution of rDNA loci.…”

Section: Systematics Of Festuca and Lolium Based On Chromosome Studiesmentioning

confidence: 99%

“…Similar differences were found among plants of F. glaucescens and F. arundinacea , where different numbers of 5S rDNA loci were observed (Thomas et al, 1997b;our unpublished results). Recently, Loureiro et al (2007) found differences in the number of rDNA loci among the plants of several polyploid fescue species (e.a. F. summilusitana , F. duriotagana , F. rubra ssp.…”

Section: Systematics Of Festuca and Lolium Based On Chromosome Studiesmentioning

confidence: 99%

Cytogenetics of Festulolium (Festuca × Lolium hybrids)

Kopecký

Lukaszewski

Doležel

2008

Cytogenet Genome Res

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Grasses are the most important and widely cultivated crops. Among them, ryegrasses (Lolium spp.) and fescues (Festuca spp.) provide high quality fodder for livestock, are used for turf and amenity purposes, and play a fundamental role in environment protection. Species from the two genera display complementary agronomic characteristics and are often grown in mixtures. Breeding efforts to combine desired features in single entities culminated with the production of Festuca × Lolium hybrids. The so called Festuloliums enjoy a considerable commercial success with numerous cultivars registered all over the world. They are also very intriguing from a strictly cytogenetic point of view as the parental chromosomes recombine freely in hybrids. Until a decade ago this phenomenon was only known in general quantitative terms. The introduction of molecular cytogenetic tools such as FISH and GISH permitted detailed studies of intergeneric chromosome recombination and karyotyping of Festulolium cultivars. These tools were also invaluable in revealing the origin of polyploid fescues, and facilitated the development of chromosome substitution and introgression lines and physical mapping of traits of interest. Further progress in this area will require the development of a larger set of cytogenetic markers and high-resolution cytogenetic maps. It is expected that the Lolium – Festuca complex will continue providing opportunities for breeding superior grass cultivars and the complex will remain an attractive platform for fundamental research of the early steps of hybrid speciation and interaction of parental genomes, as well as the processes of chromosome pairing, elimination and recombination.

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Flow cytometric and cytogenetic analyses of Iberian Peninsula Festuca spp.

Cited by 50 publications

References 34 publications

Physical Distribution of Homoeologous Recombination in Individual Chromosomes of <i>Festuca pratensis </i>in <i>Lolium multiflorum</i>

Physical Distribution of Homoeologous Recombination in Individual Chromosomes of <i>Festuca pratensis </i>in <i>Lolium multiflorum</i>

Phylogenetic Relationships in the Festuca-Lolium Complex (Loliinae; Poaceae): New Insights from Chloroplast Sequences

Cytogenetics of Festulolium (<i>Festuca </i>×<i> Lolium</i> hybrids)

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