Flow cytometric detection of herpes simplex virus type 2 infected and transformed cells by immunoenzymatic and by indirect immunofluorescence staining

Goolsby, Charles L.; Docherty, John J.; Todd, Paul

doi:10.1002/cyto.990090205

Cited by 10 publications

(4 citation statements)

References 18 publications

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“…HSV antigens have also been detected in HSV-1, HSV-2, and human herpesvirus 8 (HHV-8)-infected cells by FCM (117,213,300,347). After overnight amplification of clinical samples suspected to contain HSV, FCM detected virus 1 to 3 days before cytopathic effects were detected in cell culture (213).…”

Section: Cd38mentioning

confidence: 99%

Applications of Flow Cytometry to Clinical Microbiology

Alvarez-Barrientos¹,

Arroyo²,

Cantón³

et al. 2000

Clinical Microbiology Reviews

161

112

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Section: Cd38mentioning

confidence: 99%

Applications of Flow Cytometry to Clinical Microbiology

Alvarez-Barrientos¹,

Arroyo²,

Cantón³

et al. 2000

Clinical Microbiology Reviews

161

112

View full text Add to dashboard Cite

Section: Direct Detectionmentioning

confidence: 99%

Applications of Flow Cytometry to Clinical Microbiology

Álvarez-Barrientos¹,

Arroyo

Cantón³

et al. 2000

Clin Microbiol Rev

192

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SUMMARY Classical microbiology techniques are relatively slow in comparison to other analytical techniques, in many cases due to the need to culture the microorganisms. Furthermore, classical approaches are difficult with unculturable microorganisms. More recently, the emergence of molecular biology techniques, particularly those on antibodies and nucleic acid probes combined with amplification techniques, has provided speediness and specificity to microbiological diagnosis. Flow cytometry (FCM) allows single- or multiple-microbe detection in clinical samples in an easy, reliable, and fast way. Microbes can be identified on the basis of their peculiar cytometric parameters or by means of certain fluorochromes that can be used either independently or bound to specific antibodies or oligonucleotides. FCM has permitted the development of quantitative procedures to assess antimicrobial susceptibility and drug cytotoxicity in a rapid, accurate, and highly reproducible way. Furthermore, this technique allows the monitoring of in vitro antimicrobial activity and of antimicrobial treatments ex vivo. The most outstanding contribution of FCM is the possibility of detecting the presence of heterogeneous populations with different responses to antimicrobial treatments. Despite these advantages, the application of FCM in clinical microbiology is not yet widespread, probably due to the lack of access to flow cytometers or the lack of knowledge about the potential of this technique. One of the goals of this review is to attempt to mitigate this latter circumstance. We are convinced that in the near future, the availability of commercial kits should increase the use of this technique in the clinical microbiology laboratory.

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“…Flow cytometry has been used to detect HSV antigens in HSV-infected cultured cells. HSV-2-infected Vero cells and HSV-2-transformed mouse embryo cells were analyzed by scattered light and, for background immunofluorescence, by flow cytometry (71). Little difference was noted between HSV-2-infected and -transformed cells when they were studied by FALS and RALS; however, when the cells were treated with polyclonal antibodies to HSV-2 raised in goats as the primary antibody and FITC-labeled rabbit antigoat antibody as the second antibody, there was a significant difference between the fluorescently stained HSV-2-infected cells, which exhibited high fluorescence intensity, and the transformed cells, which did not.…”

Section: Sv40mentioning

confidence: 99%

Uses of flow cytometry in virology.

McSharry

1994

Clinical Microbiology Reviews

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these will be critically reviewed. The application of quantitative flow cytometry in the analysis of many other virus-infected cell systems is less well defined; however, that literature will be reviewed to demonstrate the broad applications of this technology for detecting and quantitating virus-infected cells. BRIEF DESCRIPTION OF FLOW CYTOMETRY Cytometry can be defined as the measurement of physical and/or chemical properties of cells. These measurements are often performed with a light or fluorescence microscope. Microscopy is qualitative but, with the exception of computerized image analysis (77), seldom quantitative. Flow cytometry is the measurement of the physical and/or chemical characteristics of cells while they pass single file in a fluid stream through a measuring apparatus (185). The fluorescence-activated cell sorter (FACS) was developed in the early 1970s to study isolated populations of viable cells for immunology. The original instruments were large, contained powerful dual lasers that were water cooled, and were able to analyze and sort cells. Although flow cytometry continues to function in the field of immunology, its use in other fields such as cell biology, 576

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Flow cytometric detection of herpes simplex virus type 2 infected and transformed cells by immunoenzymatic and by indirect immunofluorescence staining

Cited by 10 publications

References 18 publications

Applications of Flow Cytometry to Clinical Microbiology

Applications of Flow Cytometry to Clinical Microbiology

Applications of Flow Cytometry to Clinical Microbiology

Uses of flow cytometry in virology.

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