Flow Cytometric Determination of Light Scattering and Absorbance Properties of Starch Granules

Jones, Robert J.; Srienc, Friedrich; Roessler, Jeffrey; Paul, Steffen

doi:10.1002/star.19920440702

Cited by 17 publications

(16 citation statements)

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“…The size of the starch granules has been linked directly with the content of the starch components (Jones et al 1992;Meredith 1981). It has been reported that a smaller starch granule has a higher amylose content than a larger one (Ni et al 2012;Peng et al 1999;Zhang et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The granule size is correlated with some physicochemical and functional properties of starch such as granule crystallinity, thermal properties, pasting, swelling, baking, and solubility (D'Appolo and Gilles 1971; Kulp 1973;Langeveld et al 2000;Wong and Lelievre 1982;Zhou et al 2015). Ponte et al (1963) concluded that the percentage of B-type starch granule was significantly correlated with the flour breadmaking quality.…”

Section: Introductionmentioning

confidence: 99%

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Starch granule size distribution from twelve wheat cultivars in east China’s Huaibei region

Yan

Xia-qing

et al. 2016

Can. J. Plant Sci.

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Abstract:The granule size distribution of starch strongly influences its physicochemical properties and the functionality of wheat. Twelve wheat cultivars grown in east China's Huanghuai region were used for investigating the granule size distribution, amylose content, and their interrelationship. The results showed that the volume distribution of starch granules show the typical bimodal with peak values in the ranges of 4.44-5.36 μm and 21.7-23.82 μm, respectively. Surface area distribution of granules was also bimodal with peak values in the ranges of 2.53-3.06 μm and 19.8-21.7 μm, respectively. The limits between the two populations both occurred at 10 μm. A typical population of number distribution of granules with peak values in the range of 0.52-0.67 μm. Proportions of granules <2.6 μm, 2.6-10 μm, and 10-40 μm were in the range of 10.06-13.63%, 28.54-41.6%, and 45.4-61.3% of total volume, respectively. Proportions of granules <10 μm were in the range of 99.9% of the total number. The amylose content was significant and negatively correlated to volume percentage of granule <10 μm, and significant and positively correlated to the volume percentage of granule 22-40 μm.Key words: wheat (Triticum aestivum L.), starch, granule, size distribution.Résumé : La répartition des granules d'amidon exerce une forte influence sur les propriétés physicochimiques et la fonctionnalité du blé. Douze variétés de blé cultivées dans la région de Huanghuai, dans l'est de la Chine, ont servi à étudier la granulométrie de l'amidon, la teneur en amylose et les liens entre ces paramètres. Selon les résultats, la répartition volumique des particules d'amidon illustre la distribution bimodale usuelle, les valeurs maximales se situant respectivement entre 4,44-5,36 μm et 21,7-23,82 μm. La surface active des granules présente une distribution bimodale elle aussi, avec des valeurs maximales entre 2,53-3,06 μm et 19,8-21,7 μm, respectivement. Dans les deux cas, les seuils entre les groupes correspondent à 10 μm. Une population typique aura une distribution de granules dont les valeurs maximales se trouvent entre 0,52 μm et 0,67 μm. Les granules de moins de 2,6 μm, de 2,6-10 μm et de 10-40 μm représentent respectivement 10,06-13,63 %, 28,54-41,6 %, et 45,4-61,3 % du volume total. Les granules de moins de 10 μm constituent 99,9 % du chiffre total. La teneur en amylose présente une corrélation négative significative avec la proportion volumique de granules de moins de 10 μm et est positivement corrélée à la proportion volumique de granules de 22-40 μm. [Traduit par la Rédaction]

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Starch granule size distribution from twelve wheat cultivars in east China’s Huaibei region

Yan

Xia-qing

et al. 2016

Can. J. Plant Sci.

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“…Starch granule number was counted by flow cytometry (Jones et al, 1992). Endosperm cells were digested at 37°C overnight with 1 mL of 20 g L −1 cellulysin in 0.1 M NaOAc buffer (pH 4.7).…”

Section: Methodsmentioning

confidence: 99%

High Temperatures during Endosperm Cell Division in Maize: A Genotypic Comparison under In Vitro and Field Conditions

Commuri¹,

Jones

2001

Crop Science

126

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High temperature during endosperm cell division reduces grain yield of maize (Zea mays L.). The objective of the study was to determine if there were differences in tolerance of two inbred lines (B73 and Mo17) to exposure to brief high temperature treatments (HTTs). Beginning 5 d after pollination (DAP), kernels were exposed to a continuous 35°C temperature for either 4 or 6 d. The effects of HTTs on kernel development, ultrastructure, and sink capacity were evaluated under both in vitro and field conditions. In B73, the 4 and 6 d HTT reduced final kernel dry weights >40 to 60% under in vitro and 79 to 95% under field conditions, compared with the controls. The HTT‐induced reduction in kernel mass was due mainly to reduction in starch granule number, since by 16 DAP the endosperm cell number had recovered and was not significantly different from the controls. In contrast, in Mo17 both the number of endosperm cells and starch granules were reduced by >45 to 80% by the 4 and 6 d HTT imposed under the two growing conditions. Hence, these data and kernel ultrastructure evidence confirm that kernel development is more tolerant to high temperature in B73 than in Mo17. The difference appears to be due mainly to the ability of B73 to maintain a higher kernel sink capacity after exposure to HTT during endosperm cell division. Exploiting the differential response of these genotypes appears to be a viable approach to further elucidate the physiological basis for heat tolerance during early kernel development.

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“…Flow analysis and sorting methods have been developed since for purification of thylakoids isolated from mesophyll and bundle sheath cells of maize (84) based on differences in chlorophyll fluorescence spectra. Amyloplasts (starch grains), which are generally grouped with chloroplasts because both represent subsets of the classification of plastids, can be rapidly analyzed using flow cytometry, as described by ISAC member Friedrich Srienc and his coworkers (85). Flow analysis of mitochondria, which are non-fluorescent in their natural state, requires use of added fluorochromes or through in vivo targeting of GFP (86).…”

Section: Flow Analysis and Sorting Of Organellesmentioning

confidence: 99%

Cytometry and plant sciences: A personal retrospective

Galbraith

2004

Cytometry Pt A

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The plant kingdom is divided into the lower plants (blue-green algae, green algae, the bryophytes consisting of mosses and liverworts, and the euglenaphytes) and the higher plants (which mainly comprise the vascular plants). Higher plants are grouped into the pteridophytes (ferns) and their relatives, and the two classifications of seed plants, the angiosperms and the gymnosperms. The former comprises the monocotyledons and the dicotyledons, and the latter comprises the conifers and cycads (1). In this retrospective, I have restricted my coverage to the seed plants.

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Flow Cytometric Determination of Light Scattering and Absorbance Properties of Starch Granules

Cited by 17 publications

References 9 publications

Starch granule size distribution from twelve wheat cultivars in east China’s Huaibei region

Starch granule size distribution from twelve wheat cultivars in east China’s Huaibei region

High Temperatures during Endosperm Cell Division in Maize: A Genotypic Comparison under In Vitro and Field Conditions

Cytometry and plant sciences: A personal retrospective

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