Flow Cytometric Determination of Microbial Abundances and Its Use to Obtain Indices of Community Structure and Relative Activity

Gasol, Josep M.; Morán, Xosé Anxelu G.

doi:10.1007/8623_2015_139

Cited by 105 publications

(78 citation statements)

References 78 publications

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“…Heterotrophic bacterial abundances were estimated with a BD FACSCalibur flow cytometer equipped with a blue argon laser (488 nm) using the protocols described in Gasol and Morán (). About 1.8 ml was collected twice a day from each temperature incubation, fixed with final concentrations of 1% paraformaldehyde and 0.5% formaldehyde and frozen at −80°C.…”

Section: Methodsmentioning

confidence: 99%

Temperature sensitivities of microbial plankton net growth rates are seasonally coherent and linked to nutrient availability

Morán

Calvo‐Díaz

Arandia-Gorostidi

et al. 2018

Environmental Microbiology

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Recent work suggests that temperature effects on marine heterotrophic bacteria are strongly seasonal, but few attempts have been made to concurrently assess them across trophic levels. Here, we estimated the temperature sensitivities (using activation energies, E) of autotrophic and heterotrophic microbial plankton net growth rates over an annual cycle in NE Atlantic coastal waters. Phytoplankton grew in winter and late autumn (0.41 ± 0.16 SE d ) and decayed in the remaining months (-0.42 ± 0.10 d ). Heterotrophic microbes shared a similar seasonality, with positive net growth for bacteria (0.14-1.48 d ), while nanoflagellates had higher values (> 0.4 d ) in winter and spring relative to the rest of the year (-0.46 to 0.29 d ). Net growth rates activation energies showed similar dynamics in the three groups (-1.07 to 1.51 eV), characterized by maxima in winter, minima in summer and resumed increases in autumn. Microbial plankton E values were significantly correlated with nitrate concentrations as a proxy for nutrient availability. Nutrient-sufficiency (i.e., > 1 μmol l nitrate) resulted in significantly higher activation energies of phytoplankton and heterotrophic nanoflagellates relative to nutrient-limited conditions. We suggest that only within spatio-temporal windows of both moderate bottom-up and top-down controls will temperature have a major enhancing effect on microbial growth.

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Section: Methodsmentioning

confidence: 99%

Temperature sensitivities of microbial plankton net growth rates are seasonally coherent and linked to nutrient availability

Morán

Calvo‐Díaz

Arandia-Gorostidi

et al. 2018

Environmental Microbiology

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“…There are at least four different ways in which flow cytometric data can be used to infer ecosystem properties or environmental status (Gasol and Morán, 2015): (i) Presence/absence of specific microbial assemblages (e.g., presence of red-fluorescing cyanobacteria is generally associated with turbid low-light environments, whereas high abundances of Prochlorococcus or dominance of pico-eukaryotes with nutrient-rich environments; Stomp et al, 2007); (ii) Estimates of cytometric diversity (Li, 1997) of either pico-phytoplankton and heterotrophic prokaryotes; (iii) Population size and pigment content (e.g., temperatures lead to total phytoplankton and bacterioplankton decreases in cell size; Morán et al, 2010Morán et al, , 2015; and (iv) Ratios between populations abundance (e.g., the ratio between picocyanobacteria and eukaryotic picophytoplankters has been used to indicate nutrient levels as cyanobacteria are more likely to be abundant in low nutrient oligotrophic environments while eukaryotes tend to dominate in high nutrient conditions; CalvoDíaz et al, 2008).…”

Section: Analysis Of Planktonic Microbial Diversity By Flow Cytometrymentioning

confidence: 99%

Implementing and Innovating Marine Monitoring Approaches for Assessing Marine Environmental Status

et al. 2016

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Marine environmental monitoring has tended to focus on site-specific methods of investigation. These traditional methods have low spatial and temporal resolution and are relatively labor intensive per unit area/time that they cover. To implement the Marine Strategy Framework Directive (MSFD), European Member States are required to improve marine monitoring and design monitoring networks. This can be achieved by developing and testing innovative and cost-effective monitoring systems, as well as indicators of environmental status. Here, we present several recently developed methodologies and technologies to improve marine biodiversity indicators and monitoring methods. The innovative tools are discussed concerning the technologies presently utilized as well as the advantages and disadvantages of their use in routine monitoring. In particular, the present analysis focuses on: (i) molecular approaches, including microarray, Real Time quantitative PCR (qPCR), and metagenetic (metabarcoding) tools; (ii) optical (remote) sensing and acoustic methods; and (iii) in situ monitoring instruments. We also discuss Danovaro et al. Innovative Approaches in Marine Monitoring their applications in marine monitoring within the MSFD through the analysis of case studies in order to evaluate their potential utilization in future routine marine monitoring. We show that these recently-developed technologies can present clear advantages in accuracy, efficiency and cost.

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“…Prokaryotic cell abundance was estimated by flow cytometry as described elsewhere (Gasol and Moran, 2015). Prokaryotic cell size was estimated from the side scatter signal using the equation provided by Calvo-Díaz and Moran, (2006).…”

Section: Prokaryotic Heterotrophic Production and Abundancementioning

confidence: 99%

Deep ocean prokaryotic communities are remarkably malleable when facing long‐term starvation

Sebastián

Auguet

Restrepo‐Ortiz

et al. 2017

Environmental Microbiology

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The bathypelagic ocean is one of the largest ecosystems on Earth and sustains half of the ocean's microbial activity. This microbial activity strongly relies on surface-derived particles, but there is growing evidence that the carbon released through solubilization of these particles may not be sufficient to meet the energy demands of deep ocean prokaryotes. To explore how bathypelagic prokaryotes respond to the absence of external inputs of carbon, we followed the long-term (1 year) dynamics of an enclosed community. Despite the lack of external energy supply, we observed a continuous succession of active prokaryotic phylotypes, which was driven by recruitment of taxa from the seed bank (i.e., initially rare operational taxonomic units [OTUs]). A single OTU belonging to Marine Group I of Thaumarchaeota, which was originally rare, dominated the microbial community for ∼ 4 months and played a fundamental role in this succession likely by introducing new organic carbon through chemolithoautotrophy. This carbon presumably produced a priming effect, because after the decline of Thaumarchaeota, the diversity and metabolic potential of the community increased back to the levels present at the start of the experiment. Our study demonstrates the profound versatility of deep microbial communities when facing organic carbon deprivation.

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Flow Cytometric Determination of Microbial Abundances and Its Use to Obtain Indices of Community Structure and Relative Activity

Cited by 105 publications

References 78 publications

Temperature sensitivities of microbial plankton net growth rates are seasonally coherent and linked to nutrient availability

Temperature sensitivities of microbial plankton net growth rates are seasonally coherent and linked to nutrient availability

Implementing and Innovating Marine Monitoring Approaches for Assessing Marine Environmental Status

Deep ocean prokaryotic communities are remarkably malleable when facing long‐term starvation

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