Flow Cytometric Determination of the Expression of gp 51 Protein of Bovine Leukaemia Virus in Experimentally Infected Sheep

Szczotka, M.

doi:10.2478/bvip-2014-0055

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…Mammary gland immunity is largely dependent upon neutrophilic functions. Many studies have investigated the effects of BLV infection on lymphocyte subsets in infected animals (9,29,30), B cell vitality (11,28,31) and neutrophil functions (10,28). Bovine leukaemia virus can be the reason for changes in milk production and fertility in cows.…”

Section: Discussionmentioning

confidence: 99%

Expression of bovine leukaemia virus (BLV) gp51 protein in blood and milk cells of cows with leukosis

Szczotka

Kuźmak

2022

Journal of Veterinary Research

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Introduction Bovine leukaemia virus (BLV) is the retroviral causative agent of enzootic bovine leukosis, the most common neoplastic disease of cattle and a serious problem worldwide. Its diagnosis is commonly by tests for antibodies recognising the p24 capsid protein and structural glycoprotein (gp) 51. With flow cytometry recently having come to veterinary immunology, applications for it may now include BLV. The study determined BLV gp51 expression in blood and milk lymphocytes of naturally infected cows by flow cytometry. Material and Methods Nineteen Polish Black and White Lowland breed cows aged 4–9 years and naturally infected with BLV and ten uninfected counterparts had blood and milk sampled and cultured. The immunological status of the animals was confirmed with ELISA and PCR. Dual-colour flow cytometry analysis was performed with specific monoclonal antibodies for lymphocyte cluster of differentiation (CD) markers and gp51 viral envelope protein and conjugates labelled with fluorescein isothiocyanate or phycoerythrin. Bovine leukaemia virus gp51 was confirmed in lymphocytes by immunofluorescence with anti-gp51 monoclonal antibodies. Results The gp51 antigen was detected in blood and milk lymphocytes of infected cows, but the percentage of cells expressing it in milk was much lower than in blood. A depleted number of CD4+ lymphocytes, an augmented number of CD8+ lymphocytes, a lower ratio of CD4+ to CD8+ and a proliferation of CD19+ immunoglobulin M+ cells were also found. Conclusion These proliferated cells were immature, gave no sign of a tendency to differentiation and were characterised by prolonged vitality.

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Section: Discussionmentioning

confidence: 99%

Expression of bovine leukaemia virus (BLV) gp51 protein in blood and milk cells of cows with leukosis

Szczotka

Kuźmak

2022

Journal of Veterinary Research

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“…Tetraploid DI was determined in control cell line 1301. In our earlier investigations (Szczotka et al 2007) numerous sister chromatid exchanges (SCEs) in metaphasal chromosomes of BLV-infected lymphocytes were found. The obtained results indicated that BLV may cause chromosomal disorders (mutagenesis) in blood lymphocytes of leukaemic cattle.…”

Section: Discussionmentioning

confidence: 89%

Polish Journal of Veterinary Sciences

Szczotka

Kocki

Iwan

et al. 2019

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Telomeres are repetitive sequence structures at the ends of chromosomes. They consist of the double stranded DNA repeats followed by the short single stranded DNA. In humans and other verterbrates the telomeric sequence is composed of tandem of TTAGGG repeats. With each cells division telomeres shorten by up to 200 base pairs. Telomerase is an enzyme responsible for continuous cell growth and is repressed in most somatic cells, except proliferating progenitor cells, but in more than 85% of cancer cells telomerase expression is observed. Tumour cells with metastatic potential may demonstrate a high telomerase activity, allowing cells to escape from the inhibition of cell proliferation due to shortened telomeres. Determination of telomerase expression was performed with the use of PCR ELISA in samples isolated from bovine leukaemia virus (BLV) infected cows. Telomerase activity was found in almost all investigated samples. The relative telomerase activity (RTA) was higher in infected cows than in healthy animals and the differences were statistically significant (α=0.05). In blood lymphocytes of BLV-infected cows the mean values of telomerase expression determined in real-time PCR were 3534.12 copies, in the healthy group there were 1010.10 copies and these differences were also statistically significant. For telomere length evaluation the Telomere PNA/FITC FISH and Telomere PNA/FITC FISH for flow cytometry were used. The mean fluorescence intensity of telomere sequences calculated on the surface of interphase nuclei of leukaemic blood lymphocytes was lower than that in the control animals and the difference was statistically significant. The mean length of telomeres in BLV-infected and healthy cows was 31. 63 ± 12.62 and 38.4 ± 4.03, (p=0.112), respectively.

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Flow Cytometric Determination of the Expression of gp 51 Protein of Bovine Leukaemia Virus in Experimentally Infected Sheep

Cited by 2 publications

References 28 publications

Expression of bovine leukaemia virus (BLV) gp51 protein in blood and milk cells of cows with leukosis

Expression of bovine leukaemia virus (BLV) gp51 protein in blood and milk cells of cows with leukosis

Polish Journal of Veterinary Sciences

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