Flow cytometric discrimination of various phycobilin‐containing phytoplankton groups in a hypertrophic reservoir

Becker, Annette; Meister, Armin; Wilhelm, Christian

doi:10.1002/cyto.10104

Cited by 50 publications

(43 citation statements)

References 34 publications

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“…Acclimation processes of the photosynthetic apparatus of phytoplankton due to environmental changes can affect fluorometric analysis (Beutler et al 2003). Another difficulty is the differentiation of Cryptophyta and their common companions, the cyanobacteria (Becker et al 2002). As they both can contain phycoerythrin (PE), this can lead to errors in the identification of red cyanobacteria.…”

Section: Introductionmentioning

confidence: 99%

Algorithms and practical fluorescence models of the photosynthetic apparatus of red cyanobacteria and Cryptophyta designed for the fluorescence detection of red cyanobacteria and cryptophytes

Beutler

Wiltshire

Reineke

et al. 2004

Aquat. Microb. Ecol.

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In fluorometric phytoplankton analysis, the detection of red cyanobacteria is hampered by acclimation processes of the cyanobacterial photosynthetic apparatus and spectral interferences with Cryptophyta. In order to overcome these problems, a simplified energy distribution model accounting for energy pathways in the red cyanobacterial photosynthetic apparatus and the apparatus of Cryptophyta was developed. Mathematical equations were derived that enabled calculation of the pigment content of Cryptophyta and red cyanobacteria in the same sample. Phytoplankton samples were excited with 7 excitation wavelengths and measured at 4 detection wavelengths (600, 620, 650 and 685 nm) in vivo.

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Section: Introductionmentioning

confidence: 99%

Algorithms and practical fluorescence models of the photosynthetic apparatus of red cyanobacteria and Cryptophyta designed for the fluorescence detection of red cyanobacteria and cryptophytes

Beutler

Wiltshire

Reineke

et al. 2004

Aquat. Microb. Ecol.

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“…This prompted us to examine the feasibility of ␦ 13 C-signature analysis by compound-specific isotope-ratio mass spectrometry (GC-combustion-IRMS: Hayes et al 1989;Pel et al 1997) of algal fractions selected from natural phytoplankton employing FCM cell sorting. Pigment fluorescence normally provides sufficient resolution in FCM to distinguish predominant populations (Hofstraat et al 1991;Becker et al 2002). However, only 1 Corresponding author (pel19@kabelfoon.nl).…”

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confidence: 99%

Using the hidden isotopic heterogeneity in phyto‐ and zooplankton to unmask disparity in trophic carbon transfer

Pel

Hoogveld

Floris

2003

Limnology & Oceanography

103

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In this study, we show that natural phototrophic populations can be probed individually for their in situ ␦ 13 C signature by linking fluorescence-activated cell sorting and isotope-ratio mass spectrometry (IRMS) using in-line pyrolytic methylation. This novel methodology greatly improved the resolution in discriminating and tracing the differential carbon (C) pathways at the base of the pelagic food web in the cyanobacteria-dominated Lake Loosdrecht (The Netherlands). Our analysis revealed the co-occurrence of phytoplankton taxa differing by 6-10‰ in ␦ 13 C. Predominant micro-and mesozooplankton species reflected this difference as the result of preferential grazing and/ or selective digestion. Flow cytometric (FCM) retrieval of phytoplankton ␦ 13C signatures, applied in conjunction with 13 C-carbonate labeling, also enabled an assessment of in situ population-specific growth rates. Diatoms and green algae exhibited up to ninefold higher growth rates than those for cyanobacterial species. The coexistence of phytoplankton populations widely differing in ␦ 13 C, standing stock, and turnover time has important implications for the interpretation of C transfer in pelagic food webs. Our approach disclosed a disproportional impact on trophic cascades by numerically minor phototrophs that otherwise would have gone unnoticed. Despite the abundance of cyanobacterial-derived C, the zooplankton largely rely on eukaryotic algae for growth. Rotifers take a central position in passing on this algal C to the cyclopoid copepod populations in the lake. The bosminid-dominated cladoceran population uses both the cyanobacterial-and algal-derived C in approximately equal shares.

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“…Although fluorescence emission does not give any information on the concentration of phycocyanin in the water, flow cytometry could be adapted to provide an early cyanobacterial warning system (Dziallas et al 2011). There are several reports that highlight this possibility (Becker 2002;Dennis 2011). Higher cyanobacterial cell concentration will result in elevated values of fluorescence emission (Sode et al 1991).…”

Section: Phycocyanin Fluorescence Measurementmentioning

confidence: 99%

Flow cytometry as a valuable tool to study cyanobacteria:A mini-review

Poniedziałek

Falfushynska

Rzymski

2017

Limnological Review

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Flow cytometry (FCM) is routinely used in medical and veterinary diagnostics although it is also widely applied in environmental studies, including phytoplankton investigations. Cyanobacteria are wide-spread photosynthetic microorganisms that attract attention due to their ecology and potential toxicity. Therefore, novel research tools are being applied in their investigation. This paper characterizes FCM as a technique that enables photopigments (chlorophylls and phycocyanin) expressed by cyanobacteria to be excited and their emission to be subsequently detected. This feature not only allows cells to be counted in a rapid manner but also enables a wide range of potential applications in ecological and biochemical studies. The main advantages of FCM, such as rapid, automatic and precise measurements requiring small sample volumes, are also discussed in this paper along with challenges including analyses of filamentous cyanobacteria and signal overlapping. It is expected that FCM will continue to be used in some fields of cyanobacterial studies.

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Flow cytometric discrimination of various phycobilin‐containing phytoplankton groups in a hypertrophic reservoir

Cited by 50 publications

References 34 publications

Algorithms and practical fluorescence models of the photosynthetic apparatus of red cyanobacteria and Cryptophyta designed for the fluorescence detection of red cyanobacteria and cryptophytes

Algorithms and practical fluorescence models of the photosynthetic apparatus of red cyanobacteria and Cryptophyta designed for the fluorescence detection of red cyanobacteria and cryptophytes

Using the hidden isotopic heterogeneity in phyto‐ and zooplankton to unmask disparity in trophic carbon transfer

Flow cytometry as a valuable tool to study cyanobacteria:A mini-review

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