Flow Cytometric Enumeration of Reticulocyte in the Peripheral Blood from Canine infected with <i>Babesia gibsoni</i>

Uemura, Takashi; Suzuki, Shinichiro; Ohnishi, Takafumi

doi:10.1111/j.1439-0450.1990.tb01084.x

Cited by 5 publications

(3 citation statements)

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“…Several authors have confirmed this correlation for samples containing normal or high percentages of reticulocytes (1,3,(5)(6)(7)(8)(9)(10)(11)24,25,27,31,44,45). Davis and Bigelow described the use of fluorescence intensity of the reticulocyte population a s a maturity index to monitor erythropoiesis in recovering bone marrow transplant patients (10).…”

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Reticulocyte quantification by flow cytometry, image analysis, and manual counting

et al. 1992

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Reticulocyte counting by flow cytometry with thiazole orange was compared to manual or automated counting of new methylene blue stained blood smears. Forty-nine samples were compared for manual counting from randomly chosen clinical samples. Two hundred and eighty-nine samples from bone marrow transplant patients were compared during the period before and through chemo-irradiation and engraftment. The slopes of correlation plots were less than 1 when flow cytometric data were the dependent variable, suggesting that thiazole orange is less sensitive than new methylene blue. In a third study, 407 samples from bone marrow transplant patients were compared after increasing the thiazole orange concentration. The reticulocyte fluorescence distribution was divided into four groups of the brightest (youngest) 40, 60, 80, and 100% of reticulocytes. The slopes from regression analysis were 0.25, 0.49, 0.78, and 1.14, respectively. This demonstrates that thiazole orange is more sensitive than new methylene blue because the window of analysis includes an increased fraction of mature reticulocytes. In addition, the precision of each assay was measured. The rank order of precision from high to low was flow cytometry > image analysis > > manual counting. 0 1992 Wiley-Liss, Inc.

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“…For a single flow cytometric procedure, sources of inaccuracy (misidentification) should be algorithm dependent and due to extraneous cells or debris entering a preset fluorescence window. This aspect of reticulocyte analysis has received some attention recently (23,32,44,45).…”

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Reticulocyte quantification by flow cytometry, image analysis, and manual counting

et al. 1992

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“…The sensitivity of flow cytometry for documentation of acute babesial infections parallels that of conventional light microscopy, with a correlation coefficient as high as 0.97 when used in combination with fluorescent nucleic acid staining 107,109,110 . Flow cytometry has also successfully documented chronic infection, with a higher sensitivity than conventional light microscopy, but lower sensitivity than indirect fluorescent antibody (IFA) testing 111 …”

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confidence: 97%

Clinical management of canine babesiosis

Ayoob

Hackner

Prittie

2010

Journal of Veterinary Emergency and Critical Care

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Flow cytometric analysis ofEimeria tenella sporozoite populations exposed to salinomycin sodium in vitro: A comparative study using light and electron microscopy and an in vitro sporozoite invasion-inhibition test

Raether¹,

Mehlhorn

Hofmann³

et al. 1991

Parasitol Res

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Eimeria tenella sporozoites exposed to 100, 70, 60 and 50 micrograms salinomycin sodium (SAL)/ml medium 199 at 41 degrees C and then stained with propidium iodide/fluorescein diacetate were analysed by means of flow cytometry (FCM). After 20 min exposure, they showed dose-dependent alterations in their size and shape, i.e. ballooning of most cells, and enhanced intracellular esterase activity as compared with untreated controls. After longer exposure periods (40 and 70 min), inflated cells gradually changed into shrivelled or crumpled, nonviable ones, thereby showing a gradual decrease in esterase activity and a gradual loss of membrane integrity (RFA+). As compared with untreated controls, sporozoites treated with 10 micrograms SAL/ml showed negligible RFA+ values (0.4%-2%), whereas those exposed to 1 and 0.1 microgram SAL ml and to the solvent dimethylsulfoxide (DMSO, 1%) did not, even after 70 min exposure. Slight to severe structural changes manifesting as an extremely wavy surface (1 microgram SAL/ml), vacuolization of the cytoplasm, distension or destruction of the mitochondrion and rupture of cell membranes (10 micrograms SAL/ml) were seen not only at higher SAL concentrations but also (rarely) at lower ones. The ability of sporozoites to invade primary chick-kidney cells was significantly inhibited by 70, 60 and 50 micrograms SAL/ml. In general, there were close relationships between findings obtained using FCM, electron microscopy and an invasion-inhibition test. The results indicate that FCM is a reliable and sensitive technique for characterizing the parasiticidal effects on and the possible mode of action of drugs in free coccidian sporozoites.

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Flow Cytometric Enumeration of Reticulocyte in the Peripheral Blood from Canine infected with Babesia gibsoni

Cited by 5 publications

References 4 publications

Reticulocyte quantification by flow cytometry, image analysis, and manual counting

Reticulocyte quantification by flow cytometry, image analysis, and manual counting

Clinical management of canine babesiosis

Flow cytometric analysis ofEimeria tenella sporozoite populations exposed to salinomycin sodium in vitro: A comparative study using light and electron microscopy and an in vitro sporozoite invasion-inhibition test

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