Flow cytometric evaluation of leukocyte subpopulations in the follicular fluids of infertile patients

Lachapelle, Marie-Hélène; Hemmings, Robert; Roy, Denis‐Claude; Falcone, T.; Miron, Pierre

doi:10.1016/s0015-0282(16)58327-7

Cited by 64 publications

(12 citation statements)

References 22 publications

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“…In patients with endometriosis (stage I/II), compared with groups of idiopathic infertility and tubal factor, flow cytometric evaluation in FF revealed higher levels of CD14 + macrophages/monocytes which could lead to altered cytokine levels, leading to dysregulation of folliculogenesis and thereby impacting fertility (Lachapelle et al 1996). These CD14 + cells were not further characterized into macrophage or monocyte subsets.…”

Section: Macrophages In Ff Of Patients With Endometriosismentioning

confidence: 94%

“…FF consists of several immune cell subsets and cytokines. Within FF immune cells such as NK cells, T-and B lymphocytes, dendritic cells (DCs) and macrophages have been found (Lachapelle et al 1996, Takaya et al 1997, Fainaru et al 2012. In general, most studies use pre-ovulatory FF collected during IVF cycles with ovarian hyperstimulation for their analyses.…”

Section: Immunological Substances In Human Ffmentioning

confidence: 99%

“…The presence of macrophages in FF, obtained after hCG injection during IVF cycles with ovarian hyperstimulation, are described in several studies (Lachapelle et al 1996, Smith et al 2005. Macrophages can be classified into different functional subsets, with specific roles in vascularization, tissue remodeling and inflammation (Wu et al 2004, Sica & Mantovani 2012.…”

Section: Macrophages In Human Ffmentioning

confidence: 99%

“…The presence of T and B subsets in FF has been described in several heterogeneous groups of infertile patients with different indications and comparing stimulated IVF with natural cycle IVF using markers as CD3, CD4, CD8, CD14, CD15, CD19, CD20, CD27, CD45 and CD57 (Hill et al 1989, Lachapelle et al 1996, Vujisic et al 2004, Wu et al 2007, Qin et al 2016, Kollmann et al 2017. However, the function of these cells in FF remains largely unknown.…”

Section: T and B Lymphocytes And Subpopulations In Human Ffmentioning

confidence: 99%

“…In patients with superficial peritoneal endometriosis (stage I/II) undergoing IVF treatment, higher levels of NK cells have been reported in FF compared to patients without endometriosis. Approximately 20% of CD56 + NK cells expressed CD16, which indicates that the increase in NK cells was caused predominantly by the proangiogenic NK subset (CD16 -) (Lachapelle et al 1996). Endometriosis is an angiogenesis dependent condition; thus, it is not surprising that there is an abundance of proangiogenic NK cells.…”

Section: Nk Cells In Ff Of Patients With Endometriosismentioning

confidence: 99%

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Is there an immune modulating role for follicular fluid in endometriosis? A narrative review

et al. 2020

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Follicular fluid (FF) surrounds the granulosa cell–oocyte complex and is one of the mediating factors in the communication between the cells within the follicle. Literature reveals that human FF and its components are key factors to the success of natural fertilization. Among other substances, FF consists of multiple cytokines and immune cells, including interleukin 6 (IL6), IL12, sHLA-G, macrophages, NK cells and lymphocytes. Together, these cells and cytokines might influence the oocyte–granulosa–cell complex. Altered balances of immune content might be involved in changes on folliculogenesis, oocyte maturation, oocyte quality and ovulation. Furthermore, these altered balances are possibly involved in infertility associated with immune-mediated diseases such as endometriosis. The aim of this narrative review is to elaborate on the function and contents of FF and its immunological profile in patients with endometriosis. A comprehensive literature search was performed for the published literature on FF (immune) contents, FF function and FF content alterations in endometriosis patients. In FF of patients with endometriosis, elevated levels of macrophages and several cytokines have been reported. The role of specific immune cells in FF and a clarification of the biological mechanism in healthy women and endometriosis patients remain largely unknown. Future studies in this field will give us more insight in the role of FF immune cells and the effect of altered balances in patients with endometriosis.

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Section: Macrophages In Ff Of Patients With Endometriosismentioning

confidence: 94%

Section: Immunological Substances In Human Ffmentioning

confidence: 99%

Section: Macrophages In Human Ffmentioning

confidence: 99%

Section: T and B Lymphocytes And Subpopulations In Human Ffmentioning

confidence: 99%

Section: Nk Cells In Ff Of Patients With Endometriosismentioning

confidence: 99%

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Is there an immune modulating role for follicular fluid in endometriosis? A narrative review

et al. 2020

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Activation of cGAS/STING Drives Inflammation and Cellular Senescence of Macrophages in Ovarian Endometrioma Induced by Endometriotic Cyst Fluid

Ye,

Sun,

Cai

et al. 2024

Advanced Biology

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Ovarian endometrioma (OE) is a common gynecological condition characterized by the formation of “chocolate cysts”. Recent research indicates that the cyst fluid acts as a “toxic environment” for the ovary and plays a significant role in the development of OE, with macrophages being pivotal. However, the specific molecular and cellular mechanisms of it are not fully understood. In this study, clinical samples are integrated, single‐cell sequencing, in vivo and in vitro experimental models to comprehensively investigate the effects of OE fluid on ovarian function and the mechanisms of it. Combined with bioinformatics analysis and experimental validation, the findings demonstrate that OE fluid can cause ovarian function decline, which associated with inflammatory response, and mitochondrial dysfunction and cellular senescence, while activating the cGAS/STING signaling pathway. As a STING inhibitor, H‐151 effectively alleviates ovarian dysfunction, inflammatory state and cell apoptosis induced by OE fluid. Furthermore, it is also discovered that H‐151 can inhibit OE fluid‐induced mitochondrial dysfunction and cellular senescence. These findings provide important theoretical and experimental foundations for further research and development of STING inhibitors as potential drugs for treating ovarian dysfunction.

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Proteomic analysis of follicular fluid from women with and without endometriosis: New therapeutic targets and biomarkers

Turco

Cordeiro

Lopes

et al. 2013

Molecular Reproduction Devel

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Endometriosis is a gynecological disease that affects women of reproductive age. The protein profiles of women with endometriosis who were able or unable to achieve pregnancy and women without endometriosis who did achieve pregnancy were compared in this study. The follicular fluid was collected from 21 patients undergoing in vitro-fertilization treatment, according to the following groups: nine women in the control group (Group C), four women with endometriosis who achieved pregnancy (Group E.P), and eight women with endometriosis who did not achieve pregnancy (Group E.NP). Follicular fluid proteins were separated using 2D-electrophoresis, and their spots were compared, excised, and submitted to LC-ESI-MS/MS for proteins identification. The analysis showed 29 differentially expressed spots among the groups, and from these, 21 proteins were identified. Analysis showed some functional enrichment in the E.P group, including response to oxidative stress and apoptosis, while the E.NP group showed functions related to response to reactive oxygen species and positive regulation of apoptosis. These data suggest that endometriosis leads to differential protein expression in the follicular fluid, which can influences the outcome of pregnancy. These proteins may be potential targets for better diagnostics and new therapeutic intervention in affected women, as well as assisting in comprehending the physiopathologic mechanisms underlying endometriosis.

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Flow cytometric evaluation of leukocyte subpopulations in the follicular fluids of infertile patients

Cited by 64 publications

References 22 publications

Is there an immune modulating role for follicular fluid in endometriosis? A narrative review

Is there an immune modulating role for follicular fluid in endometriosis? A narrative review

Activation of cGAS/STING Drives Inflammation and Cellular Senescence of Macrophages in Ovarian Endometrioma Induced by Endometriotic Cyst Fluid

Proteomic analysis of follicular fluid from women with and without endometriosis: New therapeutic targets and biomarkers

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