Flow Cytometric Identification of Immunophenotypically Aberrant T-Cell Clusters on Skin Shave Biopsy Specimens From Patients With Mycosis Fungoides

Horna, Pedro; Kurant, Danielle E.; Sokol, Lubomir; Sotomayor, Eduardo M.; Moscinski, Lynn C.; Glass, L. Frank

doi:10.1309/ajcpwe2hbfcgdids

Cited by 7 publications

(11 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CD26 overexpression was previously described in 30–73% of MF cases . We demonstrated that CD26 expression is related to different outcomes (secondary SS or t‐MF) with CD26‐negative expression related to a high risk of progression.…”

Section: Discussionsupporting

confidence: 60%

“…In our series, a loss of surface CD3 expression or CD3 dim was found in 44% (12/27) of cases. Abnormal CD3 dim was previously described in SS and MF skin biopsies in 55–72% of cases. Other notable changes in pan‐T antigen in this series were a loss of or diminished expression of CD7 in 92.6% (25/27) of cases and a loss of CD2 described in 20% of SS cases and 11% (3/27) of cases of this series .…”

Section: Discussionmentioning

confidence: 69%

“…The loss of CD7 expression was previously found in circulating peripheral blood T cells in SS and MF skin biopsies in 57–83% of cases . CD7 loss in T CD4+ lymphocytes is sometimes difficult to interpret because CD4+/CD7− T cells exist in normal subjects; this subpopulation can increase in cases of inflammation, particularly in inflammatory skin lesions .…”

Section: Discussionmentioning

confidence: 97%

“…As diagnoses have been mostly based on the loss of pan‐T markers, positive expression of CD158k (KIR3DL2) was demonstrated to be specific to neoplastic Sezary cells . Inversely, FCM performed on the skin has been poorly studied and only series with small numbers were reported . In contrast to SS, MF skin neoplastic cells have been shown to express CD26 bright in 30–73% of cases .…”

mentioning

confidence: 99%

See 3 more Smart Citations

Usefulness of Flow Cytometry for the Detection of Cutaneous Localization in Malignant Hematologic Disorders

Maître

Le-Page

Comoz

et al. 2019

Cytometry Part B Clinical

View full text Add to dashboard Cite

Background Histological evaluation of malignant hematologic involvement of the skin can be challenging and needs an extended immunohistochemistry panel. We assessed the ability of flow cytometry (FCM) to detect neoplastic cell subsets in skin biopsies as a useful tool that supplements the histological examination in a complementary way. Methods Two hundred and forty‐three consecutive skin biopsies were retrospectively analyzed between April 2012 and July 2017. Results Among them, 147 samples, corresponding to 128 patients, were analyzed at diagnosis. Eighty‐seven patients had erythrodermic inflammatory dermatoses, and 41 patients had cutaneous hematologic neoplasms. Cutaneous T‐cell lymphomas were the most frequent disorders, accounting for 70% of cases (29/41). Cutaneous B‐cell lymphoma was found in only 17% of cases (7/41) and immature hematologic malignancies in 5% (2/41). Three patients had secondary skin involvement. The sensitivity of FCM skin biopsy analysis was 78.1% (32/41). Among the 243 samples, 27 patients had mycosis fungoides (MF) or Sezary syndrome (SS) with available FCM data. A loss of CD26 expression was identified in 92% of cases of transformed MF or SS versus 40% of cases of non‐transformed MF (P = 0.0057 χ²). Among the 12 MF patients with negative CD26 expression, six progressed to SS versus none in the positive group (50% vs. 0% P = 0.0168 χ²). Conclusions FCM analysis of the skin biopsies is a sensitive method and a useful tool for improving the sensitivity of diagnosis of hematologic skin neoplasms. Among the MF patients, a loss of CD26 expression could be a marker of higher risk of progression. © 2019 International Clinical Cytometry Society

show abstract

Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 97%

mentioning

confidence: 99%

See 2 more Smart Citations

Usefulness of Flow Cytometry for the Detection of Cutaneous Localization in Malignant Hematologic Disorders

Maître

Le-Page

Comoz

et al. 2019

Cytometry Part B Clinical

View full text Add to dashboard Cite

show abstract

“…10 The T-LPDs may be set in a polymorphic inflammatory background with eosinophils, neutrophils, plasma cells, and histiocytes similar to mixed cellularity or nodular sclerosis-type CHL. In addition, the RSC-like cells can coexpress CD30 (Figure, F), CD15, and PAX5, whereas CHL may have aberrant T-antigen expression and is CD15 À in a subset of cases.…”

Section: Flow Cytometrymentioning

confidence: 99%

Nodal Involvement by CD30+ Cutaneous Lymphoproliferative Disorders and Its Challenging Differentiation From Classical Hodgkin Lymphoma

Lezama

Gratzinger

2018

Archives of Pathology &Amp; Laboratory Medicine

View full text Add to dashboard Cite

Primary cutaneous lymphomas are defined as non-Hodgkin lymphomas that present in the skin with no evidence of extracutaneous disease at the time of diagnosis. Mycosis fungoides is the most common type of primary cutaneous T-cell lymphoma, representing almost 50% of primary cutaneous T-cell lymphomas, and primary cutaneous CD30+ T-cell lymphoproliferative disorders are the second most common group (30%). Transformed mycosis fungoides is usually CD30+ and can involve multiple nodal sites; other primary cutaneous CD30+ T-cell lymphoproliferative disorders can also involve draining regional nodes. Nodal involvement by CD30+ T-cell lymphoproliferative disorders can mimic classical Hodgkin lymphoma, which can aberrantly express T-cell antigens. The aim of this article is to briefly review salient clinical, histologic, immunophenotypic, and molecular features that can be used to distinguish lymph node involvement by CD30+ cutaneous T-cell lymphomas and lymphoproliferative disorders from classical Hodgkin lymphoma, a clinically important differential diagnosis that represents a challenging task for the pathologist.

show abstract

Sézary syndrome and mycosis fungoides: An overview, including the role of immunophenotyping

Pulitzer

Horna

Almeida

2020

Cytometry Part B Clinical

Self Cite

View full text Add to dashboard Cite

This review discusses the definition and major categories of cutaneous T-cell lymphoma, Sézary syndrome and mycosis fungoides, and the role of immunophenotyping in their diagnosis. The following key points are raised: (a) Sézary syndrome and mycosis fungoides cells most often have a characteristic CD3+ CD4+ CD7− and/or CD26− immunophenotype. (b) This immunophenotype is not specific, but can assist in the distinction from non-neoplastic T cells and other subtypes of mature T-cell neoplasm.

show abstract

Flow Cytometric Identification of Immunophenotypically Aberrant T-Cell Clusters on Skin Shave Biopsy Specimens From Patients With Mycosis Fungoides

Abstract: FC is capable of identifying putative neoplastic cells on SSB specimens from patients with MF. Bright homogeneous CD26 expression is a common and previously undescribed immunophenotypic aberrancy on MF skin infiltrates.

Cited by 7 publications

References 35 publications

Usefulness of Flow Cytometry for the Detection of Cutaneous Localization in Malignant Hematologic Disorders

Usefulness of Flow Cytometry for the Detection of Cutaneous Localization in Malignant Hematologic Disorders

Nodal Involvement by CD30+ Cutaneous Lymphoproliferative Disorders and Its Challenging Differentiation From Classical Hodgkin Lymphoma

Sézary syndrome and mycosis fungoides: An overview, including the role of immunophenotyping

Contact Info

Product

Resources

About