Flow-Cytometric Immune Function Methodology for Human Peripheral Blood Dendritic Cells

Selective developmental defects of cord blood antigen-presenting cell subsets

“…Stimulation of cytokine production by DC and monocytes was carried out essentially as described [12]. Briefly, LPS at 1 g/ml (from Escherichia coli, serotype 026:B6, Sigma) and Brefeldin A (Sigma) in DMSO (Sigma) at 10 g/ml were added to aliquots of 750 l of whole blood in 15-ml conical polypropylene tubes (Falcon).…”