Flow cytometric monitoring of the bacterial phenotypic diversity in aquatic ecosystems

Hong, Jin-Kyung; Kim, Soo Bin; Ahn, Seok Hyun; Choi, Yongjoo; Lee, Tae Kwon

doi:10.1007/s12275-021-1443-7

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…In addition, we found that the REU scores of OLP patients were associated with increase of Rothia in saliva increased and decrease of Porphyromonas on buccal mucosa, indicating that the change in the proportion of Rothia and Porphyromonas may signify the degree of severity of OLP. Studies of dental plaque and saliva from healthy and diseased subjects have identified the genus Rothia as commensal member of human oral microbiota (Baker et al, 2021; Kang et al, 2006). The most focused member of the genus Porphyromonas is Porphyromonas gingivalis associated with human periodontitis (Darveau, 2010).…”

Section: Discussionmentioning

confidence: 99%

Preliminary analysis of mucosal and salivary bacterial communities in oral lichen planus

Deng

Pan

et al. 2023

Oral Diseases

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ObjectiveTo characterize the bacterial community from different oral niches (buccal mucosa and saliva) in oral lichen planus (OLP) patients.Subjects and MethodsThis preliminary study analyzed site‐specific (mucosa and saliva) microbial landscape of 20 OLP patients and 10 healthy controls.ResultsThe microbial diversity was similar between OLP patients and healthy controls in both salivary and mucosal communities. However, the topological properties of co‐occurrence networks of salivary and mucosal microbiome were different between healthy controls and OLP patients. SparCC analysis inferred three and five keystone taxa in the salivary and mucosal microbial networks of healthy controls, respectively. However, in the salivary and mucosal bacterial networks of OLP patients, only one hub OTU and three OTUs were identified as keystone taxa, respectively. In addition, analysis of community cohesion revealed that mucosal microbial community in OLP patients had lower stability than that in healthy controls. In final, correlation assay showed that the clinical severity of OLP was positively associated with the relative abundance of Rothia in saliva but negatively associated with that of Porphyromonas on mucosa.ConclusionsThe salivary and mucosal bacterial communities of OLP patients differ in terms of composition, the genera associated with OLP severity, and co‐occurrence patterns.

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Section: Discussionmentioning

confidence: 99%

Preliminary analysis of mucosal and salivary bacterial communities in oral lichen planus

Deng

Pan

et al. 2023

Oral Diseases

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“…The phenotypic diversity was assessed by partitioning the denoised data from the FSC × FITC plot into 100 × 100 bins, based on previous research ( Hong et al, 2021b ). The R software (version 3.6.2) with identical scripts as those used in a previous study ( Hong et al, 2021a ) was used to process the data. The alpha diversity index (Shannon’s diversity index) and beta diversity were calculated using the R package “vegan” with the FCM feature tables.…”

Section: Methodsmentioning

confidence: 99%

Phenotypic shifts induced by environmental pre-stressors modify antibiotic resistance in Staphylococcus aureus

Wee,

Lyou,

Nishu

et al. 2023

Front. Microbiol.

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IntroductionEscalating prevalence of antibiotic resistance in Staphylococcus aureus has necessitated urgent exploration into the fundamental mechanisms underlying antibiotic resistance emergence, particularly in relation to its interaction with environmental stressors. This study aimed to investigate the effects of environmental stressors prior to antibiotic exposure on the antibiotic resistance of S. aureus.MethodsWe used Raman spectroscopy and flow cytometry to measure prior stress-induced phenotypic alterations of S. aureus, and identified the association between phenotypic shifts and the antibiotic resistance.ResultsThe results revealed a multifaceted relationship between stressors and the development of antibiotic resistance. The stressors effectuate distinct phenotypic diversifications and subsequently amplify these phenotypic alterations following antibiotic treatments, contingent upon the specific mode of action; these phenotypic shifts in turn promote the development of antibiotic resistance in S. aureus. This study’s findings demonstrated that the presence of pre-stress conditions triggered an augmentation of resistance to vancomycin (VAN), while concurrently attenuating resistance to norfloxacin. Marked shifts in Raman peaks associated with lipids and nucleic acids demonstrated correlations with elevated survival rates following VAN treatment.ConclusionConsequently, these observations indicate that pre-stress conditions “prime” bacterial cells for differential responses to antibiotics and bear significant implications for formulating clinical therapeutic strategies.

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Assessing long-term ecological impacts of PCE contamination in groundwater using a flow cytometric fingerprint approach

Hong,

Kim,

Wee

et al. 2024

Science of The Total Environment

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Flow cytometric monitoring of the bacterial phenotypic diversity in aquatic ecosystems

Cited by 3 publications

References 19 publications

Preliminary analysis of mucosal and salivary bacterial communities in oral lichen planus

Preliminary analysis of mucosal and salivary bacterial communities in oral lichen planus

Phenotypic shifts induced by environmental pre-stressors modify antibiotic resistance in Staphylococcus aureus

Assessing long-term ecological impacts of PCE contamination in groundwater using a flow cytometric fingerprint approach

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