2002
DOI: 10.1038/sj.bjc.6600457
|View full text |Cite
|
Sign up to set email alerts
|

Flow cytometric quantification of tumour endothelial cells; an objective alternative for microvessel density assessment

Abstract: Assessment of microvessel density by immunohistochemical staining is subject to a considerable inter-observer variation, and this has led to variability in correlation between microvessel density and clinical outcome in different studies. In order to improve the method of microvessel density measurement in tumour biopsies, we have developed a rapid, objective and quantitative method using flow cytometry on frozen tissues. Frozen tissue sections of archival tumour material were enzymatically digested. The singl… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

3
17
0

Year Published

2004
2004
2023
2023

Publication Types

Select...
6

Relationship

0
6

Authors

Journals

citations
Cited by 23 publications
(20 citation statements)
references
References 11 publications
3
17
0
Order By: Relevance
“…This result was confirmed by the classical assessment of microvessel density by CD31 staining and counting of blood vessels in adjacent cryosections (medullary 95 vessels/mm 2 versus ductal 58 vessels/mm 2 , P Ͻ 0,02). Also for the detection of endothelial cells, a significant correlation between flow cytometric and histochemical techniques was observed (r ϭ 0.658, P Ͻ 0.0001), as was described before in other tissues (10).…”
Section: Resultssupporting
confidence: 49%
See 4 more Smart Citations
“…This result was confirmed by the classical assessment of microvessel density by CD31 staining and counting of blood vessels in adjacent cryosections (medullary 95 vessels/mm 2 versus ductal 58 vessels/mm 2 , P Ͻ 0,02). Also for the detection of endothelial cells, a significant correlation between flow cytometric and histochemical techniques was observed (r ϭ 0.658, P Ͻ 0.0001), as was described before in other tissues (10).…”
Section: Resultssupporting
confidence: 49%
“…Next to the infiltrate, the amount of endothelial cells was also quantified by flow cytometry according to the earlier described method (10). Interestingly, a significantly higher number of endothelial cells was observed in medullary breast carcinoma (P Ͻ 0.01, Fig.…”
Section: Resultsmentioning
confidence: 99%
See 3 more Smart Citations