1994
DOI: 10.1007/bf01240138
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Flow-cytometrically monitored chromatin in situ testing in the breast cell line H184A1N4

Abstract: Previous investigations in human precancerous and cancerous tissues identified subsets of cells that were different in respect to a heparin-induced increase in fluorescence intensity (IFI) monitored by flow cytometry. We suggested that differences in IFI were due to different chromatin types in the cells and related to different transcriptional capacities. This study is to prove our suggestion. Heparin-induced IFI was measured in the human breast cell line H184A1N4 cultured under different, defined growth cond… Show more

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Cited by 1 publication
(3 citation statements)
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“…Chromatin in situ tests applying heparin as a chromatin probe were carried out in non-fixed cells as described [44]. Briefly, unfixed cells were stained with Ethidium Bromide/Mithramycin [47] and the DNA patterns were recorded before (t = 0) and after (t = 1, 4, 7 min) the addition of heparin (7.5 U per ml cell suspension) (Weddel Pharmaceutical Ltd., London).…”
Section: Histopathological Analysismentioning
confidence: 99%
See 2 more Smart Citations
“…Chromatin in situ tests applying heparin as a chromatin probe were carried out in non-fixed cells as described [44]. Briefly, unfixed cells were stained with Ethidium Bromide/Mithramycin [47] and the DNA patterns were recorded before (t = 0) and after (t = 1, 4, 7 min) the addition of heparin (7.5 U per ml cell suspension) (Weddel Pharmaceutical Ltd., London).…”
Section: Histopathological Analysismentioning
confidence: 99%
“…Different accessibility of chromatin to fluorescence dyes reflects different chromatin types in cells. They are related to different transcriptional capacities: sensitive cells are assumed to be transcriptionally inactive, resistant cells to be active [24,44]. Not all cell parameters were calculable in each case.…”
Section: Histopathological Analysismentioning
confidence: 99%
See 1 more Smart Citation