Flow cytometry-based algorithm to analyze the anti-fixed Toxoplasma gondii tachyzoites IgM and IgG reactivity and diagnose human acute toxoplasmosis

Silva-dos-Santos, Priscila Pinto; Barros, Geisa Baptista; Mineo, José Roberto; Silva, Deise Aparecida de Oliveira; Menegaz, Mauro Hygino Weinert; Serufo, José Carlos; Dietze, Reynaldo; Martins-Filho, Olindo de Assis; Lemos, Elenice Moreira

doi:10.1016/j.jim.2012.02.001

Cited by 8 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Venous blood samples were collected from subjects selected at baseline and stored at −80 °C until analysis. T. gondii tachyzoite (RH strain) suspension was prepared following instructions described in 68 . To assess immunoglobulin production, fixed T. gondii tachyzoites were used in a flow cytometry-based assay.…”

Section: Methodsmentioning

confidence: 99%

“…To assess immunoglobulin production, fixed T. gondii tachyzoites were used in a flow cytometry-based assay. IgM and IgG titers were measured in the 1:16000 dilution with a cutoff of 80% for IgM and 20% for IgG, and experiments were performed in 96-well plates with a slightly modified protocol compared with the one described in 68 . More detailed information regarding blood collection and processing, tachyzoite preparation, and immunofluorescence by flow cytometry can be found in the Supplementary Methods.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Toxoplasma gondii infection on cognition, symptoms, and response to digital cognitive training in schizophrenia

et al. 2022

View full text Add to dashboard Cite

Studies indicate that neuroscience-informed digital cognitive training can remediate cognitive impairments in schizophrenia, but the factors contributing to these deficits and response to treatment remain unclear. Toxoplasma gondii is a neuroinvasive parasite linked to cognitive decline that also presents a higher prevalence in schizophrenia. Here, we compared the cognition and symptom severity of IgG seropositive (TOXO+; n = 25) and seronegative (TOXO−; n = 35) patients who participated in a randomized controlled trial of digital cognitive training. At baseline, TOXO+ subjects presented lower global cognition than TOXO− (F = 3.78, p = 0.05). Specifically, TOXO+ subjects showed worse verbal memory and learning (F = 4.48, p = 0.03), social cognition (F = 5.71, p = 0.02), and higher antibody concentrations were associated with increased negative (r = 0.42, p = 0.04) and total (r = 0.40, p = 0.04) schizophrenia symptoms. After training, the TOXO+ group showed higher adherence to the intervention (X2 = 9.31, p = 0.03), but there were no differences in changes in cognition and symptoms between groups. These findings highlight the association between seropositivity to T. gondii and deteriorated cognition and symptoms in schizophrenia. Further research is needed to assess the specific efficacy of digital cognitive training on this population.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effects of Toxoplasma gondii infection on cognition, symptoms, and response to digital cognitive training in schizophrenia

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Among the selected studies, the use of samples such as serum, whole blood, colostrum and amniotic fluid was observed, and these were associated with different methods of detection of T. gondii and its respective markers. Table 1 presents the general characteristics of the selected articles [6][7][8][9][10][11][12][13][14][15][16][17][18] .…”

Section: General Characteristics Of the Study And Total Resultsmentioning

confidence: 99%

Molecular and serological diagnosis of toxoplasmosis: a systematic review and meta-analysis

Souza

Siqueira

Ribeiro

et al. 2023

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

Toxoplasmosis is an infection of vast worldwide distribution whose etiologic agent is Toxoplasma gondii. This disease can cause problems ranging from mild symptoms to serious conditions, such as encephalitis, miscarriage and blindness. Therefore, it is of utmost importance to perform a diagnosis with reproducible techniques in order to obtain a good prognosis. The aim of this review was to analyze the efficiency of toxoplasmosis diagnostic techniques based on sensitivity and specificity results. Five research platforms in English language were used (Eric, Elsevier, Google Scholar, PubMed and SciELO), which contained data on the diagnosis of toxoplasmosis. The search and selection were performed for studies published prior to June 2021. The search resulted in the inclusion of 13 articles published from 2005 to 2020. The data revealed the use of different samples in the standardization of techniques such as serum, total blood, colostrum and amniotic fluid. The flow cytometry, lateral flow immunoassay and qPCR techniques showed 100% sensitivity, whereas the ELISA, western blotting, qPCR and RE-LAMP techniques achieved 100% specificity. Significantly, the qPCR and LAMP techniques were more accurate when the likelihood ratio was assessed. The meta-analysis identified that ISAGA and western blotting have low sensitivity values and LIASON, ELFA and ELISA, using a silica bioconjugate, also have low specificity values. It was noted that a wide range of methods have high values of sensitivity and specificity. Therefore, the choice of the method will be based on the conditions and its financial viability.

show abstract

“…Because of the difficulty of isolating the parasite, and the generally asymptomatic nature of the primary infection, the diagnosis of acquired toxoplasmosis in immunocompetent subjects is based on serologic tests: seroconversion to 2 isotypes (IgM and IgG antibodies) or a significant increase in the IgG titer plus IgM positivity [17]. Specific IgM is considered as an acute-phase marker.…”

Section: Discussionmentioning

confidence: 99%

Acute Toxoplasmosis Mimicking Melanoma Metastases: Review of Conditions Causing False-Positive Results on <sup>18</sup>F-FDG PET/CT

Ivanova

Glatz²,

Zippelius³

et al. 2012

Dermatology

View full text Add to dashboard Cite

Invasive malignant melanoma is the most common fatal form of skin cancer. Fluorine-18-fluorodeoxyglucose positron emission tomography-computed tomography demonstrates a very high sensitivity and specificity for the detection of melanoma metastases. Here, we report an unusual case of toxoplasma lymphadenitis in a male adult patient mimicking a malignant cervical lymphadenopathy. Toxoplasmosis is a zoonosis caused by the intracellular parasite Toxoplasma gondii, which is usually asymptomatic in immunocompetent hosts.

show abstract

Flow cytometry-based algorithm to analyze the anti-fixed Toxoplasma gondii tachyzoites IgM and IgG reactivity and diagnose human acute toxoplasmosis

Cited by 8 publications

References 34 publications

Effects of Toxoplasma gondii infection on cognition, symptoms, and response to digital cognitive training in schizophrenia

Effects of Toxoplasma gondii infection on cognition, symptoms, and response to digital cognitive training in schizophrenia

Molecular and serological diagnosis of toxoplasmosis: a systematic review and meta-analysis

Acute Toxoplasmosis Mimicking Melanoma Metastases: Review of Conditions Causing False-Positive Results on <sup>18</sup>F-FDG PET/CT

Contact Info

Product

Resources

About