Flow Cytometry

Jacobsen, Charlotte Nexmann

doi:10.1006/rwfm.1999.0665

Cited by 3 publications

(5 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus far, soils-based studies have largely focused on factors affecting Norse agricultural production such as home-field irrigation requirements (Adderley & Simpson, 2006) and human-induced soil erosion (Jacobsen & Jakobsen, 1986).…”

Section: Norse-inuit Interaction and Landscape Change In Southern Grementioning

confidence: 99%

Norse–Inuit interaction and landscape change in southern Greenland? A geochronological, Pedological, and Palynological investigation

et al. 2011

View full text Add to dashboard Cite

Ruins representing both medieval Norse and Inuit (Thule culture) settlements can be found together on the coast at Sandhavn (59°59' N, 44°46' W), Greenland. The site presents a rare opportunity to investigate the character of past contact and interaction between these two peoples. Soils-based, radiocarbon, and palynological analyses demonstrate the creation of hortic anthrosols within Norse home-fields used between the mid-11th and late 14th centuries A.D. Irrigation channels have been identified within the home-fields, while rising grass pollen influx reveals intensification in hay production over the period ca. A.D. 1260-1350 despite climatic deterioration setting in around this time. Floor deposits and wall packing from an Inuit winter house returned dates of cal. A.D. 1220-1290 (2 s), yet no direct landscape-based evidence for Inuit activity could be determined. Although the exact nature of the relationship between Norse and Thule at Sandhavn remains unclear, the role of this site as a harbor and possible trading area may have attracted Inuit settlers keen to participate in European trade networks.

show abstract

Section: Norse-inuit Interaction and Landscape Change In Southern Grementioning

confidence: 99%

Norse–Inuit interaction and landscape change in southern Greenland? A geochronological, Pedological, and Palynological investigation

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Phenotypic data that may be collected using FCM include enzyme activities, antibiotic resistance, cell size, refractive index, presence of para‐sporal crystals, numbers of cells comprising each CFU and genome size (Reardon and Scheper 1993; Porter et al. 1996; Jacobsen and Jakobsen 1999; Nebe‐von‐Caron et al. 2000).…”

Section: Common Themes In Bacillus Cereus Research and The Utility Ofmentioning

confidence: 99%

“…Excellent descriptions of the range of dyes available to the microbiologist are published in a number of reviews (Davey et al 1999;Jacobsen and Jakobsen 1999;Alvarez-Barrientos et al 2000;Nebe-von-Caron et al 2000;Sincock andRobinson 2001 Shapiro 2003). DNA content is estimated by staining of cells with fluorochromes such as Hoechst 33342 that specifically bind to DNA (Lloyd 1999).…”

Section: Growthmentioning

confidence: 99%

“…Any technique capable of simultaneously measuring up to 12 parameters per cell (such as FCM) is bound to enhance the collection of phenotypic data that can be then used to further characterize and categorize strains. Phenotypic data that may be collected using FCM include enzyme activities, antibiotic resistance, cell size, refractive index, presence of para-sporal crystals, numbers of cells comprising each CFU and genome size (Reardon and Scheper 1993;Porter et al 1996;Jacobsen and Jakobsen 1999;Nebe-von-Caron et al 2000). FCM can also be used to perform serotyping, another helpful technique in the classification of micro-organisms (McClelland and Pinder 1994;Edwards et al 1996;Porter et al 1996;Barbesti et al 2000;Sincock and Robinson 2001).…”

Section: Taxonomymentioning

confidence: 99%

“…Excellent descriptions of the range of dyes available to the microbiologist are published in a number of reviews (Davey et al. 1999; Jacobsen and Jakobsen 1999; Alvarez‐Barrientos et al. 2000; Nebe‐von‐Caron et al.…”

Section: Common Themes In Bacillus Cereus Research and The Utility Ofmentioning

confidence: 99%

See 2 more Smart Citations

The potential of flow cytometry in the study of Bacillus cereus

Cronin

Wilkinson

2010

Journal of Applied Microbiology

View full text Add to dashboard Cite

Summary Flow cytometry (FCM) is a rapid method allowing the acquisition of multiparametric data from thousands of individual cells within a sample. As well as measuring the intrinsic light scattering properties of cells, a plethora of fluorescent dyes may be employed to yield information on macromolecule content, surface antigens present or physiological status. Despite FCM’s indispensability within other fields e.g. immunology, it is underutilized within microbiological research. In this review, a strong case is presented for the potential of FCM in the study of Gram‐positive spore‐former, Bacillus cereus. Previous reports where FCM was successfully used in the study of B. cereus are reviewed along with relevant studies involving other members of the genus. Under headings reflecting common research themes associated with B. cereus, specific instances where FCM has generated novel data, providing a unique insight into the organism, are discussed. Further applications are posited, based on the authors’ own research with FCM and B. cereus and work extant in the broader field of microbial cytometry. The authors conclude that, while the expense of equipment and reagents is an undeniable disadvantage, FCM is a technique capable of generating significantly novel data and allows the design and execution of experiments that are not possible with any other technique.

show abstract

Transit along the vas deferens results in a high percentage of filiform spermatozoa with compacted chromatin in the rooster (

Bernal

Behnamifar²,

Álvarez-Rodríguez³

et al. 2022

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

The present work aimed to evaluate the chromatin compaction of rooster spermatozoa along the male reproductive tract, and to study the vas deferens lining cells, potentially involved in sperm maturation. Chromomycin A3 (CMA3) was used to determine the chromatin compaction of spermatozoa from testis (T), proximal (including epididymis, V1), intermediate (V2) and distal (V3) vas deferens, and ejaculate (E). Six Birchen Leonesa roosters were used. E was obtained in vivo by dorso-ventral massage. V1, V2 and V3 sperm were obtained post mortem (six pairs of vasa deferentia), by flushing. T was obtained by washing the testes, cut in halves. The fixed cells were stained with CMA3 and propidium iodide for flow cytometry assessment. Results showed higher (P < 0.01) median fluorescence intensity (lower chromatin compaction) of T (693.8 ± 30.2) than V1 (546.3 ± 17.7), V2 (515.1 ± 12.1), V3 (517.6 ± 12.3) and E (491.4 ± 16.1). Regarding the percentage of intensely stained cells, T differs (P < 0.05) from V2, V3 and E, V1 differs (P < 0.05) from V3 and E, while V2, V3 and E do not differ. The histological analysis revealed secretory capacity of the vas deferens. Our findings specified that the transit though the vas deferens results in high percentage of compacted chromatin spermatozoa in E.

show abstract

Flow Cytometry

Cited by 3 publications

References 4 publications

Norse–Inuit interaction and landscape change in southern Greenland? A geochronological, Pedological, and Palynological investigation

Norse–Inuit interaction and landscape change in southern Greenland? A geochronological, Pedological, and Palynological investigation

The potential of flow cytometry in the study of Bacillus cereus

Transit along the vas deferens results in a high percentage of filiform spermatozoa with compacted chromatin in the rooster (

Contact Info

Product

Resources

About