Flow dynamics control the location of sprouting and direct elongation during developmental angiogenesis

Ghaffari, Siavash; Leask, Richard L.; Jones, Elizabeth A. V.

doi:10.1242/dev.128058

Cited by 59 publications

(72 citation statements)

References 24 publications

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“…In addition to mechanical forces induced by collective cell migration, mechanical forces derived from blood flow will further contribute to control angiogenesis [121]. For instance, once the level of increasing shear stress reaches a certain threshold, the formation of sprouts is promoted [122].…”

Section: Junction Remodeling In Angiogenesismentioning

confidence: 99%

Cell–cell junctional mechanotransduction in endothelial remodeling

Dorland

Huveneers

2016

Cell. Mol. Life Sci.

150

142

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The vasculature is one of the most dynamic tissues that encounter numerous mechanical cues derived from pulsatile blood flow, blood pressure, activity of smooth muscle cells in the vessel wall, and transmigration of immune cells. The inner layer of blood and lymphatic vessels is covered by the endothelium, a monolayer of cells which separates blood from tissue, an important function that it fulfills even under the dynamic circumstances of the vascular microenvironment. In addition, remodeling of the endothelial barrier during angiogenesis and trafficking of immune cells is achieved by specific modulation of cell–cell adhesion structures between the endothelial cells. In recent years, there have been many new discoveries in the field of cellular mechanotransduction which controls the formation and destabilization of the vascular barrier. Force-induced adaptation at endothelial cell–cell adhesion structures is a crucial node in these processes that challenge the vascular barrier. One of the key examples of a force-induced molecular event is the recruitment of vinculin to the VE-cadherin complex upon pulling forces at cell–cell junctions. Here, we highlight recent advances in the current understanding of mechanotransduction responses at, and derived from, endothelial cell–cell junctions. We further discuss their importance for vascular barrier function and remodeling in development, inflammation, and vascular disease.

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Section: Junction Remodeling In Angiogenesismentioning

confidence: 99%

Cell–cell junctional mechanotransduction in endothelial remodeling

Dorland

Huveneers

2016

Cell. Mol. Life Sci.

150

142

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“…Shear stress occurs simultaneously with the onset of a heartbeat, which occurs early in embryogenesis (Table 1). The initiation of WSS plays an important role in the development of all components of the cardiovascular system, including remodeling of blood vessels [21,22 ■ ], hematopoiesis [18,23], angiogenesis [24,25], as well as lymphangiogenesis [26,27]. In the following section, we focus on the specific requirements of shear stress in aspects of heart development.…”

Section: Mechanical Forces and Mechanotransduction In Cardiovascular mentioning

confidence: 99%

Pulling on my heartstrings

McCormick

Tzima

2016

Current Opinion in Hematology

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Purpose of review Endothelial cells line the surface of the cardiovascular system and display a large degree of heterogeneity due to developmental origin and location. Despite this heterogeneity, all endothelial cells are exposed to wall shear stress (WSS) imparted by the frictional force of flowing blood, which plays an important role in determining the endothelial cell phenotype. Although the effects of WSS have been greatly studied in vascular endothelial cells, less is known about the role of WSS in regulating cardiac function and cardiac endothelial cells. Recent findings Recent advances in genetic and imaging technologies have enabled a more thorough investigation of cardiac hemodynamics. Using developmental models, shear stress sensing by endocardial endothelial cells has been shown to play an integral role in proper cardiac development including morphogenesis and formation of the conduction system. In the adult, less is known about hemodynamics and endocardial endothelial cells, but a clear role for WSS in the development of coronary and valvular disease is increasingly appreciated. Summary Future research will further elucidate a role for WSS in the developing and adult heart, and understanding this dynamic relationship may represent a potential therapeutic target for the treatment of cardiomyopathies.

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“…37 Interestingly, this same sprouting behavior was recently observed in vivo in avian embryos. 41 Collectively, these findings suggest that the local interstitial flow dynamics can both determine whether sprouting occurs and specify where these sprouts originate from the parent vessel.…”

Section: Application Of the Pdms-hydrogel Hybrid Systems Has Contributedmentioning

confidence: 91%

Microfluidic approaches to the study of angiogenesis and the microcirculation

2017

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Microfluidic systems have emerged as a new class of perfusable in vitro culture models that have helped advance and refine our understanding of microvascular function. Cutting-edge microfluidic models have successfully integrated principles from quantitative analysis of vascular function, in vitro flow chambers, microfabrication techniques, and 3D tissue scaffolds. Here, we review the evolution of microfluidic systems, namely their progression from 2D planar microchannel arrays to 3D microtissue analogs, and highlight their recent contributions in elucidating the role of biomolecular transport and fluid mechanical stimuli in controlling angiogenesis. Further advancement of microfluidic systems in recapitulating tissue-level phenomena in vitro, controlling important physiochemical and biological parameters, and integrating cellular and molecular analysis will help further enhance their application within the microcirculation research community.

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Flow dynamics control the location of sprouting and direct elongation during developmental angiogenesis

Cited by 59 publications

References 24 publications

Cell–cell junctional mechanotransduction in endothelial remodeling

Cell–cell junctional mechanotransduction in endothelial remodeling

Pulling on my heartstrings

Microfluidic approaches to the study of angiogenesis and the microcirculation

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